breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
MC JC 1,587,854 Δ12 bp coding (432‑443/504 nt) ydeR ← putative fimbrial protein YdeR
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,339,065 G→C A119G (GCA→GGA)  gyrA ← DNA gyrase subunit A
MC JC 2,972,221 Δ12 bp coding (625‑636/1041 nt) tas → putative NADP(H)‑dependent aldo‑keto reductase Tas
RA 3,244,885 T→A intergenic (‑144/‑339) uxaC ← / → exuT D‑glucoronate/D‑galacturonate isomerase/hexuronate transporter
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,476,866 T→C Y185C (TAC→TGC)  fkpA ← peptidyl‑prolyl cis‑trans isomerase FkpA
MC JC 3,512,071 Δ3 bp coding (272‑274/906 nt) yhfZ ← putative DNA‑binding transcriptional regulator YhfZ
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 696738 696862 125 24 [23] [0] 53 [metU]–glnW [metU],glnW
* * ÷ NC_000913 1196283 1211428 15146 26 [23] [21] 30 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 1978495 1979270 776 100 [0] [0] 69 insB‑5–insA‑5 insB‑5,insA‑5
* * ÷ NC_000913 3423770–3424525 3424525 1–756 26 [22] [21] 27 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 696863 =0 (0.000)46 (0.520)
+GAAGAAACAATC
22/252 2.1 58.0% intergenic (‑33/+2) glnW/glnU tRNA‑Gln/tRNA‑Gln
?NC_000913 = 697134 73 (0.750)intergenic (‑1/+379) metT/asnB tRNA‑Met/asparagine synthetase B
* ? NC_000913 = 12994980 (0.000)75 (0.800) 50/268 0.4 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 19784940 (0.000)100 (1.030) 61/276 0.2 100% intergenic (‑297/+24) flhD/insB‑5 DNA‑binding transcriptional dual regulator FlhD/IS1 protein InsB
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 1979271 =0 (0.000)68 (0.700) 46/276 0.6 100% intergenic (‑56/‑482) insA‑5/uspC IS1 protein InsA/universal stress protein C
?NC_000913 2101745 = NA (NA)intergenic (‑1/+150) wbbL/insH‑7 interrupted rhamnosyltransferase WbbL/IS5 transposase and trans‑activator
* ? NC_000913 3363001 =156 (1.610)141 (1.460) 69/274 0.1 64.1% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)166 (1.710) 84/276 0.0 97.1% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 5 (0.050)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 4542682 =48 (0.490)27 (0.300) 24/258 2.0 37.3% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 46 (0.510)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 454269048 (0.490)53 (0.580) 39/258 0.8 53.8% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 46 (0.510)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit