breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 223,648 A→G intergenic (+240/‑123) gmhB → / → rrsH D‑glycero‑beta‑D‑manno‑heptose‑1,7‑bisphosphate 7‑phosphatase/16S ribosomal RNA
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 881,995 Δ1 bp coding (740/759 nt) deoR ← DNA‑binding transcriptional repressor DeoR
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,059,312 C→T G19G (GGC→GGT torA → trimethylamine N‑oxide reductase 1
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,513,646 T→A I277N (ATC→AAC)  ydcT → putative ABC transporter ATP‑binding protein YdcT
RA 1,597,281 A→G pseudogene (806/5422 nt) yneO ← AIDA‑I family autotransporter YneO
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
JC JC 2,480,678 IS5 (+) +4 bp coding (1496‑1499/1539 nt) emrY ← tripartite efflux pump membrane subunit EmrY
RA 2,818,760 T→C intergenic (‑115/+201) serV ← / ← csrA tRNA‑Ser/carbon storage regulator
RA 3,077,978 A→C A294A (GCT→GCG cmtA ← mannitol‑specific PTS enzyme IICB component CmtA
RA 3,337,427 G→C N155K (AAC→AAG mlaC ← intermembrane phospholipid transport system ‑ periplasmic binding protein
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,370,371 Δ1 bp coding (666/690 nt) nanE ← putative N‑acetylmannosamine‑6‑phosphate epimerase
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,977,710 (A)6→5 coding (186/1080 nt) rffT → 4‑acetamido‑4,6‑dideoxy‑D‑galactose transferase
RA 3,977,712 C→T S63L (TCG→TTG)  rffT → 4‑acetamido‑4,6‑dideoxy‑D‑galactose transferase
RA 3,977,715 4 bp→ACA coding (191‑194/1080 nt) rffT → 4‑acetamido‑4,6‑dideoxy‑D‑galactose transferase
RA 3,977,720 G→A E66K (GAA→AAA)  rffT → 4‑acetamido‑4,6‑dideoxy‑D‑galactose transferase
RA 4,238,711 A→G I360V (ATC→GTC)  yjbH → YjbH family protein
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 696729 696862 134 26 [25] [0] 57 [metU]–glnW [metU],glnW
* * ÷ NC_000913 1196255 1211435 15181 27 [24] [25] 26 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 2818121–2818143 2818255–2818202 60–135 26 [25] [25] 26 [argZ]–[argY] [argZ],[argY]
* * ÷ NC_000913 3423768–3424536 3424536 1–769 28 [21] [24] 29 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 696863 =0 (0.000)53 (0.770)
+GAAGAAACAATC
24/252 1.9 62.1% intergenic (‑33/+2) glnW/glnU tRNA‑Gln/tRNA‑Gln
?NC_000913 = 697134 71 (0.940)intergenic (‑1/+379) metT/asnB tRNA‑Met/asparagine synthetase B
* ? NC_000913 = 12994980 (0.000)91 (1.240) 55/268 0.2 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =94 (1.240)107 (1.420) 61/274 0.1 69.2% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)84 (1.110) 53/276 0.2 94.4% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 5 (0.070)coding (3907/4134 nt) rhsA rhs element protein RhsA