breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 954,589 A→G intergenic (‑123/+283) focA ← / ← ycaO formate channel FocA/ribosomal protein S12 methylthiotransferase accessory factor YcaO
RA 1,195,676 Δ1 bp coding (554/1251 nt) icd → isocitrate dehydrogenase
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,397,754 T→A D290V (GAT→GTT)  uspE ← universal stress protein with a role cellular motility
RA 1,579,335 G→A T3I (ACC→ATC)  yddA ← ABC transporter family protein YddA
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,404,587 A→T D9E (GAT→GAA nuoB ← NADH:quinone oxidoreductase subunit B
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,427,482 (A)7→6 intergenic (‑60/‑212) yjfZ ← / → ytfA protein YjfZ/putative transcriptional regulator YtfA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196277 1211428 15152 10 [7] [7] 11 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3423750–3424231 3424523–3424238 8–774 10 [6] [6] 10 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3751950 3751981 32 10 [8] [8] 11 yiaT/yiaU outer membrane protein YiaT/putative LysR‑type DNA‑binding transcriptional regulator YiaU

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)52 (1.250) 36/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =82 (1.920)79 (1.860) 47/274 0.0 65.2% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.070)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)40 (0.940) 33/276 0.2 95.2% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.050)coding (3907/4134 nt) rhsA rhs element protein RhsA