breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation annotation gene description
RA BN000925 9,791 A→G D797G (GAC→GGC)  tnpA → Tn1 transposase
RA BN000925 9,802 G→A D801N (GAC→AAC)  tnpA → Tn1 transposase
RA BN000925 10,089 A→C A896A (GCA→GCC tnpA → Tn1 transposase
RA BN000925 10,359 T→A G986G (GGT→GGA tnpA → Tn1 transposase
RA BN000925 12,239 +A intergenic (‑601/+1055) klcA ← / ← tetR KlcA putative anti‑restriction protein/tetracycline resistance repressor protein
RA BN000925 12,406 T→G intergenic (‑768/+888) klcA ← / ← tetR KlcA putative anti‑restriction protein/tetracycline resistance repressor protein
RA BN000925 12,688 T→A intergenic (‑1050/+606) klcA ← / ← tetR KlcA putative anti‑restriction protein/tetracycline resistance repressor protein
RA BN000925 15,191 G→T C381F (TGC→TTC)  tetA → tetracycline exporter protein
RA BN000925 15,561 Δ1 bp intergenic (+312/+320) tetA → / ← upf16.5 tetracycline exporter protein/hypothetical protein
RA BN000925 15,639 C→G intergenic (+390/+242) tetA → / ← upf16.5 tetracycline exporter protein/hypothetical protein
RA BN000925 15,898 +C coding (649/666 nt) upf16.5 ← hypothetical protein
RA BN000925 16,410 +T coding (137/666 nt) upf16.5 ← hypothetical protein
RA BN000925 31,028 C→G P35A (CCC→GCC)  trbP → TrbP protein
RA BN000925 36,646 A→G T113A (ACT→GCT) 
T105A (ACT→GCT) 
istA →
istA →
IS21 transposase
IS21 transposase
RA BN000925 36,655 A→G R116G (AGA→GGA) 
R108G (AGA→GGA) 
istA →
istA →
IS21 transposase
IS21 transposase
RA BN000925 38,388 A→C pseudogene (45/171 nt) upf35.8 → probable truncation of theoretical product due to sequence errors
RA BN000925 38,390 1 bp→AG pseudogene (47/171 nt) upf35.8 → probable truncation of theoretical product due to sequence errors
RA BN000925 38,393 C→T pseudogene (50/171 nt) upf35.8 → probable truncation of theoretical product due to sequence errors
RA BN000925 38,421 Δ1 bp pseudogene (78/171 nt) upf35.8 → probable truncation of theoretical product due to sequence errors
RA BN000925 38,510 Δ1 bp pseudogene (167/171 nt) upf35.8 → probable truncation of theoretical product due to sequence errors
RA BN000925 38,526 Δ1 bp intergenic (+12/‑232) upf35.8 → / → aphA probable truncation of theoretical product due to sequence errors/aminoglycoside 3'‑phospho‑transferase
RA BN000925 39,136 Δ1 bp coding (379/774 nt) aphA → aminoglycoside 3'‑phospho‑transferase
RA BN000925 39,147 Δ1 bp coding (390/774 nt) aphA → aminoglycoside 3'‑phospho‑transferase
RA BN000925 39,179 +CG coding (422/774 nt) aphA → aminoglycoside 3'‑phospho‑transferase
RA BN000925 53,069 +T intergenic (+42/+10) traM → / ← kfrC TraM protein/KfrC protein
RA BN000925 54,833 Δ1 bp intergenic (‑32/+127) kfrB ← / ← kfrA KfrB protein/KfrA coiled‑coil protein
RA BN000925 56,817 Δ1 bp coding (297/522 nt) korF ← KorF repressor protein
MC JC NC_000913 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA NC_000913 263,721 A→G V96A (GTA→GCA)  yafW ← antitoxin of the YkfI‑YafW toxin‑antitoxin pair
RA NC_000913 336,746 T→C G274G (GGT→GGC yahE → DUF2877 domain‑containing protein YahE
RA NC_000913 475,719 C→T A114V (GCG→GTG)  ybaY → PF09619 family lipoprotein YbaY
RA NC_000913 485,773 A→G T5A (ACC→GCC)  acrR → DNA‑binding transcriptional repressor AcrR
RA NC_000913 504,319 A→G G126G (GGT→GGC fsr ← fosmidomycin efflux pump
RA NC_000913 525,854 A→G D865G (GAC→GGC)  rhsD → protein RhsD
RA NC_000913 731,061 C→T G493G (GGC→GGT rhsC → rhs element protein RhsC
RA NC_000913 736,871 A→G intergenic (+172/‑29) ybfQ → / → ybfL inactive transposase YbfQ/putative transposase YbfL
RA NC_000913 802,681 A→G G265G (GGA→GGG ybhI → putative tricarboxylate transporter
RA NC_000913 883,192 A→G V66A (GTC→GCC)  ybjG ← undecaprenyl pyrophosphate phosphatase
RA NC_000913 986,795 Δ1 bp coding (188/1089 nt) ompF ← outer membrane porin F
RA NC_000913 1,068,671 A→G V390A (GTA→GCA)  rutG ← pyrimidine:H(+) symporter
RA NC_000913 1,133,465 A→G T298A (ACG→GCG)  flgE → flagellar hook protein FlgE
RA NC_000913 1,212,088 (C)8→9 intergenic (‑85/+172) iraM ← / ← ymgK anti‑adaptor protein IraM, inhibitor of sigma(S) proteolysis/protein YmgK
RA NC_000913 1,258,835 Δ1 bp coding (45/279 nt) ychH → stress‑induced protein
RA NC_000913 1,296,375 A→G F583L (TTC→CTC)  adhE ← aldehyde‑alcohol dehydrogenase
RA NC_000913 1,298,371 C→A intergenic (‑250/‑227) adhE ← / → ychE aldehyde‑alcohol dehydrogenase/putative inner membrane protein
MC JC NC_000913 1,299,499 Δ1,199 bp insH21 insH21
RA NC_000913 1,303,679 C→T Q261* (CAA→TAA)  oppB → murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
RA NC_000913 1,384,170 A→G R18R (AGA→AGG ycjX → DUF463 domain‑containing protein YcjX
RA NC_000913 1,630,547 T→C T123A (ACA→GCA)  ydfI ← putative oxidoreductase YdfI
RA NC_000913 1,640,644 G→A intergenic (‑59/+695) essQ ← / ← cspB Qin prophage; putative S lysis protein/Qin prophage; cold shock‑like protein CspB
RA NC_000913 1,779,316 T→C intergenic (‑15/‑301) ydiP ← / → ydiQ putative DNA‑binding transcriptional regulator YdiP/putative electron transfer flavoprotein subunit YdiQ
RA NC_000913 2,023,510 T→C R206R (CGT→CGC fliR → flagellar biosynthesis protein FliR
RA NC_000913 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA NC_000913 2,189,308 T→C T535A (ACC→GCC)  yehB ← putative fimbrial usher protein YehB
RA NC_000913 2,249,587 A→T F11Y (TTT→TAT)  yeiE ← putative LysR‑type transcriptional regulator YeiE
RA NC_000913 2,742,794 G→A E138K (GAA→AAA)  dgcN → diguanylate cyclase DgcN
RA NC_000913 2,781,961 T→C T256A (ACC→GCC)  ypjA ← adhesin‑like autotransporter YpjA
RA NC_000913 2,792,838 T→C V35A (GTG→GCG)  gabT → 4‑aminobutyrate aminotransferase GabT
RA NC_000913 2,830,095 T→C F95L (TTT→CTT)  gutQ → D‑arabinose 5‑phosphate isomerase GutQ
RA NC_000913 2,875,269 T→C T51A (ACC→GCC)  cysN ← sulfate adenylyltransferase subunit 1
RA NC_000913 2,992,541 (A)7→8 coding (448/1377 nt) ygeH → putative transcriptional regulator YgeH
RA NC_000913 3,001,304 (G)7→8 coding (960/2259 nt) xdhA → putative xanthine dehydrogenase molybdenum‑binding subunit XdhA
RA NC_000913 3,213,803 T→C S253P (TCT→CCT)  rpoD → RNA polymerase, sigma 70 (sigma D) factor
RA NC_000913 3,266,510 T→C T186A (ACC→GCC)  tdcA ← DNA‑binding transcriptional activator TdcA
RA NC_000913 3,290,995 G→A D728N (GAT→AAT)  yraJ → putative fimbrial usher protein YraJ
MC JC NC_000913 3,366,751 Δ295 bp IS5‑mediated yhcE[yhcF] yhcE, [yhcF]
RA NC_000913 3,440,918 T→C T38A (ACT→GCT)  rpoA ← RNA polymerase subunit alpha
RA NC_000913 3,490,674 Δ1 bp coding (156/564 nt) pabA ← aminodeoxychorismate synthase subunit 2
RA NC_000913 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA NC_000913 3,717,751 C→T L148L (CTG→TTG)  ghrB → glyoxylate reductase
RA NC_000913 3,834,319 (C)5→4 coding (219/2319 nt) yicI ← alpha‑D‑xyloside xylohydrolase
RA NC_000913 3,914,585 A→G Y206H (TAT→CAT)  glmU ← fused N‑acetylglucosamine‑1‑phosphate uridyltransferase and glucosamine‑1‑phosphate acetyltransferase
RA NC_000913 4,037,496 A→T intergenic (+161/‑23) alaT → / → rrlA tRNA‑Ala/23S ribosomal RNA
RA NC_000913 4,137,246 T→C T138A (ACC→GCC)  yijF ← conserved protein YijF
RA NC_000913 4,165,913 G→A S162S (TCG→TCA murI → glutamate racemase
RA NC_000913 4,168,472 G→A intergenic (+25/‑169) gltT → / → rrlB tRNA‑Glu/23S ribosomal RNA
RA NC_000913 4,168,475 2 bp→TG intergenic (+28/‑165) gltT → / → rrlB tRNA‑Glu/23S ribosomal RNA
RA NC_000913 4,212,973 Δ1 bp intergenic (+27/‑67) rrlE → / → rrfE 23S ribosomal RNA/5S ribosomal RNA
RA NC_000913 4,257,249 A→G E45E (GAA→GAG lexA → DNA‑binding transcriptional repressor LexA
RA NC_000913 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA NC_000913 4,345,819 A→G V503A (GTC→GCC)  fumB ← fumarase B
RA NC_000913 4,398,322 A→T H304L (CAC→CTC)  mutL → DNA mismatch repair protein MutL
RA NC_000913 4,606,095 A→G noncoding (71/87 nt) leuV ← tRNA‑Leu

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423857–3424233 3424445–3424238 6–589 14 [13] [12] 14 [rrlD] [rrlD]
* * ÷ NC_000913 4037600–4037831 4040415–4039392 1562–2816 15 [13] [13] 15 rrlA 23S ribosomal RNA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 8073360 (0.000)38 (0.720)
+G
20/428 1.6 56.4% intergenic (‑55/+97) ybhC/ybhB outer membrane lipoprotein YhbC/putative kinase inhibitor
?NC_000913 = 4171849 59 (1.110)intergenic (+93/‑208) rrfB/murB 5S ribosomal RNA/UDP‑N‑acetylenolpyruvoylglucosamine reductase
* ? NC_000913 1207790 =56 (1.050)21 (0.430) 19/398 1.5 29.4% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 49 (0.990)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment