breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
MC CP000731 1 Δ27,041 bp 100% repAUSA300HOU_pUSA300HOUMR0033 26 genes
MC NC_012417 1 Δ3,125 bp 100% USA300HOU_RS14890USA300HOU_RS14900 USA300HOU_RS14890, USA300HOU_RS15665, USA300HOU_RS14895, USA300HOU_RS14900
RA USA300TCH1516_ALE 39,378 C→A 100% D586Y (GAT→TAT)  pbp ← Beta‑lactam‑inducible penicillin‑binding protein
RA USA300TCH1516_ALE 111,512 A→G 20.1% S194S (TCA→TCG USA300HOU_RS00520 → putative lipoprotein
RA USA300TCH1516_ALE 111,516 C→A 20.0% Q196K (CAA→AAA)  USA300HOU_RS00520 → putative lipoprotein
RA USA300TCH1516_ALE 367,644 G→T 100% intergenic (‑96/‑62) nanA ← / → bglK N‑acetylneuraminate lyase/Beta‑glucoside kinase
RA USA300TCH1516_ALE 479,553 G→A 100% A194T (GCA→ACA)  USA300HOU_RS02280 → hypothetical protein
RA USA300TCH1516_ALE 638,614 G→T 100% T14K (ACA→AAA)  pdxK ← Pyridoxine kinase
RA USA300TCH1516_ALE 707,879 A→T 100% L11I (TTA→ATA)  znuC_1 ← High‑affinity zinc uptake system ATP‑binding protein ZnuC
RA USA300TCH1516_ALE 790,778 G→A 100% intergenic (+277/‑197) kipA_1 → / → ltaS KipI antagonist/Lipoteichoic acid synthase
RA USA300TCH1516_ALE 1,064,126 G→C 100% Y121* (TAC→TAG ywtF ← Putative transcriptional regulator YwtF
RA USA300TCH1516_ALE 1,232,465 G→C 100% A124P (GCG→CCG)  prkC → Serine/threonine‑protein kinase PrkC
RA USA300TCH1516_ALE 1,238,307 C→T 100% Q141* (CAA→TAA)  USA300HOU_RS06160 → hypothetical protein
RA USA300TCH1516_ALE 1,274,212 A→T 100% K205N (AAA→AAT codY → GTP‑sensing transcriptional pleiotropic repressor CodY
RA USA300TCH1516_ALE 1,318,237 T→A 100% F124Y (TTT→TAT)  miaB → tRNA‑2‑methylthio‑N(6)‑dimethylallyladenosine synthase
RA USA300TCH1516_ALE 1,380,750 C→T 28.6% intergenic (+564/‑328) opuD_1 → / → acnA Glycine betaine transporter OpuD/Aconitate hydratase A
RA USA300TCH1516_ALE 1,416,458 A→T 100% I216N (ATT→AAT)  oppD_4 ← Oligopeptide transport ATP‑binding protein OppD
RA USA300TCH1516_ALE 1,440,007 Δ1 bp 100% coding (34/201 nt) cspA_2 ← Cold shock protein CspA
RA USA300TCH1516_ALE 1,474,639 C→G 100% A9129P (GCA→CCA)  ebh_1 ← Extracellular matrix‑binding protein ebh
RA USA300TCH1516_ALE 1,481,355 G→T 100% A6890E (GCA→GAA)  ebh_1 ← Extracellular matrix‑binding protein ebh
RA USA300TCH1516_ALE 1,513,930 A→T 100% L413F (TTA→TTT USA300HOU_RS07375 → hypothetical protein
RA USA300TCH1516_ALE 1,607,132 C→T 100% E275K (GAA→AAA)  frc ← Formyl‑CoA:oxalate CoA‑transferase
RA USA300TCH1516_ALE 1,646,189 G→C 100% A155G (GCA→GGA)  ypdF ← Aminopeptidase YpdF
RA USA300TCH1516_ALE 1,744,454 C→T 100% G73D (GGC→GAC)  apt ← Adenine phosphoribosyltransferase
RA USA300TCH1516_ALE 1,770,911 T→A 100% S73C (AGT→TGT)  USA300HOU_RS08840 ← hypothetical protein
RA USA300TCH1516_ALE 1,797,772 G→C 100% R7G (CGC→GGC)  phoR ← Alkaline phosphatase synthesis sensor protein PhoR
RA USA300TCH1516_ALE 1,883,654 C→T 100% G522D (GGT→GAT)  ebh_2 ← Extracellular matrix‑binding protein ebh
RA USA300TCH1516_ALE 2,010,363 C→T 100% C146Y (TGT→TAT)  USA300HOU_RS10125 ← Putative multidrug export ATP‑binding/permease protein
RA USA300TCH1516_ALE 2,018,727:1 +A 100% coding (192/810 nt) mgt ← Monofunctional glycosyltransferase
RA USA300TCH1516_ALE 2,143,110 G→A 100% V163V (GTG→GTA USA300HOU_RS10935 → hypothetical protein
RA USA300TCH1516_ALE 2,274,965 G→C 100% E106D (GAG→GAC mtlR → Transcriptional regulator MtlR
RA USA300TCH1516_ALE 2,279,705 G→A 100% A943V (GCA→GTA)  ebh_3 ← Extracellular matrix‑binding protein ebh
RA USA300TCH1516_ALE 2,315,088 T→A 100% K61I (AAA→ATA)  ndhE ← NAD(P)H‑quinone oxidoreductase subunit 4L, chloroplastic
RA USA300TCH1516_ALE 2,342,380 A→C 100% L76V (TTG→GTG)  USA300HOU_RS11950 ← putative hydrolase
RA USA300TCH1516_ALE 2,388,866 A→G 100% intergenic (‑149/+542) sarV ← / ← moaA HTH‑type transcriptional regulator SarV/GTP 3',8‑cyclase
RA USA300TCH1516_ALE 2,434,045 C→T 24.4% G62G (GGC→GGT ybbH_3 → putative HTH‑type transcriptional regulator YbbH
RA USA300TCH1516_ALE 2,504,889 G→A 19.9% intergenic (+193/+211) USA300HOU_RS12890 → / ← gltT hypothetical protein/Proton/sodium‑glutamate symport protein
RA USA300TCH1516_ALE 2,504,894 C→T 17.1% intergenic (+198/+206) USA300HOU_RS12890 → / ← gltT hypothetical protein/Proton/sodium‑glutamate symport protein
RA USA300TCH1516_ALE 2,504,957 G→C 9.8% intergenic (+261/+143) USA300HOU_RS12890 → / ← gltT hypothetical protein/Proton/sodium‑glutamate symport protein
RA USA300TCH1516_ALE 2,504,989 T→C 10.6% intergenic (+293/+111) USA300HOU_RS12890 → / ← gltT hypothetical protein/Proton/sodium‑glutamate symport protein
RA USA300TCH1516_ALE 2,505,018 C→G 13.1% intergenic (+322/+82) USA300HOU_RS12890 → / ← gltT hypothetical protein/Proton/sodium‑glutamate symport protein
RA USA300TCH1516_ALE 2,532,298 A→T 100% T15T (ACA→ACT femA_3 → Aminoacyltransferase FemA
RA USA300TCH1516_ALE 2,679,650 G→A 100% C45Y (TGT→TAT)  ssaA2_4 → Staphylococcal secretory antigen ssaA2
RA USA300TCH1516_ALE 2,709,596 Δ1 bp 100% coding (1331/1812 nt) oatA_2 ← O‑acetyltransferase OatA
RA USA300TCH1516_ALE 2,856,087 T→A 100% P80P (CCT→CCA rpoA_2 → DNA‑directed RNA polymerase subunit alpha

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ USA300TCH1516_ALE 2176704–2181100 2181100 1–4397 2 [1] [1] 2 ilvA/USA300TCH1516_02069 L‑threonine dehydratase biosynthetic IlvA/tRNA‑Gly
* * ÷ USA300TCH1516_ALE 2241155 2241177 23 2 [1] [1] 2 murA2 UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase 2