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breseq  version 0.32.1  revision 5f2cb669173e
 mutation predictions | marginal predictions | summary statistics | genome diff | command line log  | 
| Predicted mutations | ||||||
|---|---|---|---|---|---|---|
| evidence | position | mutation | freq | annotation | gene | description | 
| RA | 111,512 | A→G | 19.0% | S194S (TCA→TCG) | USA300HOU_0110 → | possible staphylococcal tandem lipoprotein | 
| RA | 111,516 | C→A | 17.5% | Q196K (CAA→AAA) | USA300HOU_0110 → | possible staphylococcal tandem lipoprotein | 
| RA | 175,610 | T→A | 12.9% | H61Q (CAT→CAA) | cap5C → | capsular polysaccharide biosynthesis protein Cap5C | 
| RA | 367,644 | G→T | 100% | intergenic (‑96/‑20) | nanA ← / → USA300HOU_0336 | N‑acetylneuraminate lyase/possible transcriptional regulator | 
| RA | 479,553 | G→A | 100% | A194T (GCA→ACA) | USA300HOU_0458 → | hypothetical protein | 
| RA | 506,009 | C→A | 14.1% | P137Q (CCA→CAA) | amyA → | alpha, alpha‑phosphotrehalase | 
| RA | 512,643 | A→G | 64.2% | intergenic (+711/‑150) | recR → / → rrs1 | recombination protein RecR/16S ribosomal RNA | 
| RA | 522,999 | A→G | 14.5% | M1M (ATG→GTG) † | USA300HOU_0485 → | hypothetical protein | 
| RA | 638,614 | G→T | 100% | T14K (ACA→AAA) | thiD ← | phosphomethylpyrimidine kinase | 
| RA | 707,879 | A→T | 100% | L11I (TTA→ATA) | USA300HOU_0653 ← | ABC superfamily ATP binding cassette transporter, ABC protein | 
| RA | 790,778 | G→A | 100% | intergenic (+277/‑197) | USA300HOU_0741 → / → USA300HOU_0742 | possible allophanate hydrolase subunit 2/possible phosphatidylglycerol‑‑membrane‑oligosaccharide glycerophosphotransferase | 
| RA | 1,232,465 | G→C | 100% | A124P (GCG→CCG) | USA300HOU_1157 → | non‑specific serine/threonine protein kinase | 
| RA | 1,388,143 | T→C | 16.1% | L10L (CTT→CTC) | parC → | DNA topoisomerase (ATP‑hydrolyzing) ParC | 
| RA | 1,416,458 | A→T | 100% | I216N (ATT→AAT) | USA300HOU_1314 ← | oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein | 
| RA | 1,440,007 | Δ1 bp | 100% | coding (34/201 nt) | cspA ← | cold shock protein CspA | 
| RA | 1,474,639 | C→G | 100% | A9129P (GCA→CCA) | ebh ← | extracellular matrix binding protein | 
| RA | 1,481,355 | G→T | 100% | A6890E (GCA→GAA) | ebh ← | extracellular matrix binding protein | 
| RA | 1,513,930 | A→T | 100% | L413F (TTA→TTT) | USA300HOU_1379 → | hypothetical membrane protein | 
| RA | 1,646,189 | G→C | 100% | A155G (GCA→GGA) | USA300HOU_1531 ← | M24B family Xaa‑Pro dipeptidase | 
| RA | 1,682,458 | A→G | 11.5% | intergenic (‑203/+101) | phoH ← / ← USA300HOU_1573 | phosphate starvation‑inducible protein PhoH/hypothetical protein | 
| RA | 1,744,454 | C→T | 100% | G73D (GGC→GAC) | apt ← | adenine phosphoribosyltransferase | 
| RA | 1,797,772 | G→C | 100% | R7G (CGC→GGC) | USA300HOU_1679 ← | sensor histidine kinase | 
| RA | 2,010,363 | C→T | 100% | C146Y (TGT→TAT) | USA300HOU_1861 ← | ABC superfamily ATP binding cassette transporter, membrane protein | 
| RA | 2,021,817 | C→T | 23.3% | V139I (GTT→ATT) | USA300HOU_1873 ← | acyl‑CoA hydrolase | 
| RA | 2,028,233 | G→C | 19.0% | Q138E (CAA→GAA) | USA300HOU_1882 ← | hypothetical membrane protein | 
| RA | 2,108,625 | C→T | 11.3% | G454S (GGC→AGC) | USA300HOU_1971 ← | terminase large subunit | 
| RA | 2,279,705 | G→A | 100% | intergenic (+1373/+6659) | mtlD → / ← glmM | mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase | 
| RA | 2,486,221 | C→A | 13.8% | V481L (GTA→TTA) | USA300HOU_2349 ← | LctP family L‑lactate permease | 
| RA | 2,532,298 | A→T | 100% | T15T (ACA→ACT) | USA300HOU_2392 → | FemAB family peptidoglycan biosynthesis protein | 
| RA | 2,856,087 | T→A | 100% | P80P (CCT→CCA) | USA300HOU_2698 → | hypothetical protein | 
| Unassigned new junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | CP000730 | 512620 = | 16 (0.650) | 21 (1.240) +40 bp  | 
18/180 | NT | 65.5% | intergenic (+688/‑173) | recR/rrs1 | recombination protein RecR/16S ribosomal RNA | 
| ? | CP000730 | 1998278 = | NA (NA) | intergenic (‑6/+5) | USA300HOU_t0050/rrf4 | tRNA‑Val/5S ribosomal RNA | |||||