breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 39,378 C→A D586Y (GAT→TAT)  mecA ← penicillin binding protein 2 prime
RA 367,644 G→T intergenic (‑96/‑20) nanA ← / → nagC N‑acetylneuraminate lyase/possible transcriptional regulator
RA 479,553 G→A A194T (GCA→ACA)  USA300HOU_0458 → hypothetical protein
RA 638,614 G→T T14K (ACA→AAA)  thiD ← phosphomethylpyrimidine kinase
RA 707,879 A→T L11I (TTA→ATA)  mntB ← ABC superfamily ATP binding cassette transporter, ABC protein
RA 790,778 G→A intergenic (+277/‑197) kipA → / → ltaS possible allophanate hydrolase subunit 2/possible phosphatidylglycerol‑‑membrane‑oligosaccharide glycerophosphotransferase
MC JC 819,056 Δ596 bp [ytxJ] [ytxJ]
RA 1,064,126 G→C Y121* (TAC→TAG brpA ← LytR family transcriptional regulator
RA 1,232,465 G→C A124P (GCG→CCG)  prkC → non‑specific serine/threonine protein kinase
RA 1,238,307 C→T Q141* (CAA→TAA)  yloV → glycerone kinase
RA 1,274,212 A→T K205N (AAA→AAT codY → CodY family transcriptional regulator
RA 1,318,237 T→A F124Y (TTT→TAT)  miaB → 2‑methylthioadenine synthetase
RA 1,416,458 A→T I216N (ATT→AAT)  oppD ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,440,007 Δ1 bp coding (34/201 nt) cspA ← cold shock protein CspA
RA 1,474,639 C→G A9129P (GCA→CCA)  ebh ← extracellular matrix binding protein
RA 1,481,355 G→T A6890E (GCA→GAA)  ebh ← extracellular matrix binding protein
RA 1,513,930 A→T L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,607,132 C→T intergenic (+1247/+815) USA300HOU_1490 → / ← USA300HOU_1492 integrase/possible lipoprotein
RA 1,646,189 G→C A155G (GCA→GGA)  pepP ← M24B family Xaa‑Pro dipeptidase
RA 1,744,454 C→T G73D (GGC→GAC)  apt ← adenine phosphoribosyltransferase
RA 1,770,911 T→A S73C (AGT→TGT)  hemD ← uroporphyrinogen‑III synthase
RA 1,797,772 G→C R7G (CGC→GGC)  phoR ← sensor histidine kinase
RA 1,883,654 C→T G522D (GGT→GAT)  fmtB1 ← cell surface anchored protein
RA 2,010,363 C→T C146Y (TGT→TAT)  ygaD ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,018,733 (A)6→7 coding (186/810 nt) mgt ← transglycosylase
RA 2,143,110 G→A V163V (GTG→GTA USA300HOU_2026 → hypothetical membrane protein
RA 2,274,965 G→C E106D (GAG→GAC bglG → transcriptional antiterminator
RA 2,279,705 G→A intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,315,088 T→A K61I (AAA→ATA)  rhbC ← MFS family major facilitator transporter
RA 2,342,380 A→C L78V (TTG→GTG)  USA300HOU_2199 ← possible HAD superfamily hydrolase
RA 2,388,866 A→G G8G (GGA→GGG USA300HOU_2249 → hypothetical protein
RA 2,532,298 A→T T15T (ACA→ACT murN → FemAB family peptidoglycan biosynthesis protein
RA 2,679,650 G→A C45Y (TGT→TAT)  cbpD → possible secretory antigen
RA 2,709,600 (T)5→4 coding (1327/1812 nt) oatA ← possible acetyltransferase
RA 2,856,087 T→A P80P (CCT→CCA USA300HOU_2698 → hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 512620 =115 (1.280)87 (1.370)
+40 bp
54/196 0.1 51.6% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 1998278 = NA (NA)intergenic (‑6/+5) USA300HOU_t0050/rrf4 tRNA‑Val/5S ribosomal RNA