breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 318 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913354,2880TG56.8% 38.1 / 56.9 44V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
*NC_000913501,5750AC53.8% 47.8 / 63.4 39V555G (GTG→GGG) ybaLinner membrane putative NAD(P)‑binding transporter
*NC_000913354,2820TG53.5% 40.3 / 52.3 44V526G (GTT→GGT) prpEpropionate‑‑CoA ligase
*NC_0009134,378,8020AC53.3% 30.6 / 41.8 30V48G (GTA→GGA) ampCpenicillin‑binding protein; beta‑lactamase, intrinsically weak
*NC_000913987,4940TG52.8% 29.8 / 51.1 36intergenic (‑512/+91)ompF/asnSouter membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
*NC_0009131,622,8160GA51.8% 11.0 / 79.9 54intergenic (+75/+144)yneM/dgcZinner membrane‑associated protein/diguanylate cyclase, zinc‑sensing
*NC_0009131,079,4680AG50.9% 58.3 / 81.0 53D55G (GAT→GGT) putPproline:sodium symporter
*NC_0009132,511,7720AC50.0% 111.6 / 18.6 74V312G (GTG→GGG) mntHmanganese/divalent cation transporter
*NC_000913366,5260TG48.3% 86.3 / 20.6 59T329P (ACC→CCC) lacIlactose‑inducible lac operon transcriptional repressor
*NC_0009133,029,9650AC48.1% 64.7 / 20.1 52L323V (TTA→GTA) ygfTputative oxidoreductase, Fe‑S subunit/nucleotide‑binding subunit
*NC_0009133,443,2770AC47.8% 107.3 / 20.4 69V274G (GTA→GGA) secYpreprotein translocase membrane subunit
*NC_0009132,984,4410AC47.5% 96.7 / 13.2 60G384G (GGT→GGGyqeFshort chain acyltransferase
*NC_0009134,538,1090AC47.0% 92.2 / 23.2 66G219G (GGT→GGGnanMN‑acetylneuraminic acid mutarotase
*NC_0009131,487,1630CT46.5% 50.0 / 13.7 43intergenic (+200/‑72)hrpA/ydcFputative ATP‑dependent helicase/DUF218 superfamily protein, SAM‑binding
*NC_000913436,5040AC46.5% 101.7 / 25.9 71T291P (ACC→CCC) thiLthiamine monophosphate kinase
*NC_000913599,5760TG45.8% 115.6 / 25.9 72V288G (GTG→GGG) cusAcopper/silver efflux system, membrane component
*NC_0009132,921,1770AC45.6% 95.4 / 12.4 57G308G (GGT→GGGgudPputative D‑glucarate transporter
*NC_000913941,7130TG44.8% 107.7 / 19.8 67V252G (GTG→GGG) dmsAdimethyl sulfoxide reductase, anaerobic, subunit A
*NC_000913436,5160CA43.9% 78.9 / 27.8 66Q295K (CAA→AAA) thiLthiamine monophosphate kinase
*NC_0009132,921,1730TC43.9% 77.3 / 18.6 57I310V (ATT→GTT) gudPputative D‑glucarate transporter

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 120780560 (0.970)12 (0.220) 12/266 6.2 19.0% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 49 (0.880)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 1207790 =58 (0.930)9 (0.160) 9/266 7.6 14.9% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 51 (0.920)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related