breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 430 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913357,8800TC49.2% 61.6 / 29.6 65K271E (AAA→GAA) cynRtranscriptional activator of cyn operon; autorepressor
*NC_000913357,8900CA49.2% 27.4 / 42.5 63L267F (TTG→TTTcynRtranscriptional activator of cyn operon; autorepressor
*NC_0009132,421,4430AC46.2% 144.3 / 19.6 91Y40S (TAC→TCC) folXD‑erythro‑7,8‑dihydroneopterin triphosphate 2'‑epimerase and dihydroneopterin aldolase
*NC_00091329,4960TA45.5% 132.5 / 64.8 112intergenic (+301/‑155)dapB/carAdihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
*NC_0009132,984,4410AC44.2% 131.0 / 23.6 86G384G (GGT→GGGyqeFshort chain acyltransferase
*NC_0009132,511,7720AC43.8% 109.3 / 10.3 66V312G (GTG→GGG) mntHmanganese/divalent cation transporter
*NC_000913266,3580TG43.5% 97.9 / 20.6 71T66P (ACC→CCC) yafYlipoprotein, inner membrane; degP regulator; CP4‑6 prophage
*NC_000913650,4540TG41.9% 98.7 / 11.4 63T13P (ACC→CCC) citEcitrate lyase, citryl‑ACP lyase (beta) subunit
*NC_0009133,105,4990AC41.8% 151.6 / 12.3 92Y356S (TAC→TCC) mltCmembrane‑bound lytic murein transglycosylase C
*NC_0009134,378,8020AC41.6% 160.7 / 16.3 90V48G (GTA→GGA) ampCpenicillin‑binding protein; beta‑lactamase, intrinsically weak
*NC_000913650,4640AT41.3% 81.6 / 24.9 63R9R (CGT→CGAcitEcitrate lyase, citryl‑ACP lyase (beta) subunit
*NC_000913436,5160CA41.1% 74.7 / 19.8 56Q295K (CAA→AAA) thiLthiamine monophosphate kinase
*NC_0009132,248,4310AC41.0% 112.8 / 22.1 78G34G (GGT→GGGlysPlysine transporter
*NC_0009131,097,5810AG40.3% 98.3 / 13.3 63noncoding (72/88 nt)serXtRNA‑Ser
*NC_0009134,536,1970AT40.0% 124.0 / 61.1 90intergenic (+166/+417)yjhR/nanSpseudogene, helicase family; putative frameshift suppressor/9‑O‑acetyl N‑acetylneuraminic acid esterase
*NC_00091329,4860CA39.6% 188.7 / 56.1 111intergenic (+291/‑165)dapB/carAdihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
*NC_0009132,151,6860GT39.6% 42.9 / 14.3 53intergenic (+40/+25)yegJ/yegKuncharacterized protein/ser/thr phosphatase‑related protein
*NC_0009132,481,8040GA39.5% 135.8 / 29.7 81P125S (CCA→TCA) emrYputative multidrug efflux system
*NC_0009132,895,0320TC38.9% 120.3 / 20.3 73I250V (ATT→GTT) ygcQputative flavoprotein
*NC_0009131,157,2710TG38.6% 148.7 / 13.3 88G165G (GGT→GGGycfHputative DNase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =39 (0.450)29 (0.370) 24/266 3.8 48.4% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 27 (0.350)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 2473351 =88 (1.020)3 (0.040) 3/278 11.9 3.6% pseudogene (235/708 nt) oweS pseudogene, CPS‑53 (KpLE1) prophage; bacteriophage replication protein O family;Phage or Prophage Related
?NC_000913 2473361 = 79 (0.980)pseudogene (225/708 nt) oweS pseudogene, CPS‑53 (KpLE1) prophage; bacteriophage replication protein O family;Phage or Prophage Related