breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 596 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,177,7480CA48.3% 72.1 / 68.2 89E55* (GAG→TAG) nudFADP‑ribose pyrophosphatase
*NC_0009133,133,3020TG46.2% 134.2 / 18.6 80H219P (CAC→CCC) yghSputative ATP‑binding protein
*NC_000913357,8900CA45.9% 56.6 / 39.2 63L267F (TTG→TTTcynRtranscriptional activator of cyn operon; autorepressor
*NC_000913436,5160CA45.8% 77.4 / 36.4 59Q295K (CAA→AAA) thiLthiamine monophosphate kinase
*NC_00091329,4960TA45.2% 80.9 / 49.4 73intergenic (+301/‑155)dapB/carAdihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
*NC_0009132,001,9180AC44.3% 97.3 / 19.7 61intergenic (‑129/+192)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein (flagellin)
*NC_000913599,5760TG43.8% 101.6 / 22.3 65V288G (GTG→GGG) cusAcopper/silver efflux system, membrane component
*NC_0009132,434,8820TG43.3% 106.5 / 24.3 67Y252S (TAC→TCC) truAtRNA pseudouridine(38‑40) synthase
*NC_0009133,423,2870GC42.9% 109.0 / 38.2 78intergenic (+93/+136)yhdZ/rrfFputative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon
*NC_0009131,535,7890CA42.4% 99.3 / 34.9 59V24F (GTT→TTT) yddEPhzC‑PhzF family protein
*NC_0009131,825,0560GA42.2% 67.0 / 21.1 45intergenic (‑119/+84)ves/spycold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
*NC_0009131,776,2630TG42.1% 93.8 / 17.9 58V226G (GTG→GGG) ydiFputative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit
*NC_0009131,376,5050AC41.9% 115.0 / 14.6 62T158P (ACC→CCC) ycjRputative TIM alpha/beta barrel enzyme
*NC_0009132,547,7720TG41.9% 128.4 / 12.7 75V367G (GTA→GGA) murPN‑acetylmuramic acid permease, EIIBC component, PTS system
*NC_000913436,5040AC41.4% 97.6 / 17.2 60T291P (ACC→CCC) thiLthiamine monophosphate kinase
*NC_0009131,535,7850AG41.4% 83.5 / 15.9 58F25S (TTC→TCC) yddEPhzC‑PhzF family protein
*NC_000913941,7130TG41.3% 75.6 / 11.8 46V252G (GTG→GGG) dmsAdimethyl sulfoxide reductase, anaerobic, subunit A
*NC_0009131,736,7840TC41.3% 70.1 / 10.6 46G169G (GGA→GGGydhPputative MFS transporter, inner membrane protein
*NC_0009131,825,0610GA41.3% 67.2 / 25.1 46intergenic (‑124/+79)ves/spycold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
*NC_00091315,4100TG41.2% 118.2 / 11.5 69intergenic (+112/‑35)dnaJ/insL1chaperone Hsp40, DnaK co‑chaperone/IS186 transposase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 175569939 (0.510)17 (0.220) 17/298 4.0 31.2% coding (2/1413 nt) pykF pyruvate kinase I
?NC_000913 1765394 = 36 (0.470)coding (239/411 nt) menI 1,4‑dihydroxy‑2‑naphthoyl‑CoA hydrolase