breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 654 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913599,5760TG52.9% 54.3 / 102.2 68V288G (GTG→GGG) cusAcopper/silver efflux system, membrane component
*NC_000913357,8900CA50.0% 33.8 / 26.1 46L267F (TTG→TTTcynRtranscriptional activator of cyn operon; autorepressor
*NC_0009132,434,8820TG49.4% 96.7 / 35.8 77Y252S (TAC→TCC) truAtRNA pseudouridine(38‑40) synthase
*NC_000913993,9130GC49.3% 103.9 / 29.8 69G44G (GGC→GGGssuBaliphatic sulfonate ABC transporter ATPase
*NC_000913436,5160CA49.2% 63.5 / 45.1 61Q295K (CAA→AAA) thiLthiamine monophosphate kinase
*NC_000913993,9040AC47.6% 96.1 / 12.8 63G47G (GGT→GGGssuBaliphatic sulfonate ABC transporter ATPase
*NC_00091375,0790CT46.7% 54.0 / 49.8 60L134L (CTG→CTAthiBthiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein
*NC_0009133,261,0550AC45.2% 121.1 / 14.1 75V455G (GTG→GGG) tdcEpyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase
*NC_0009131,478,3240TG45.1% 126.3 / 35.8 82G33G (GGT→GGGynbBputative CDP‑diglyceride synthase
*NC_0009132,678,0390TG45.1% 111.8 / 28.7 71N108T (AAC→ACC) yphEputative sugar ABC transporter ATPase
*NC_000913357,8800TC44.9% 64.5 / 12.4 49K271E (AAA→GAA) cynRtranscriptional activator of cyn operon; autorepressor
*NC_0009131,764,7850TA44.3% 84.5 / 37.9 71intergenic (‑399/+149)sufA/ydiHFe‑S cluster assembly protein/uncharacterized protein
*NC_0009132,326,4500TG43.2% 139.6 / 17.3 74G114G (GGT→GGGatoBacetyl‑CoA acetyltransferase
*NC_0009132,434,8610GT43.2% 129.4 / 42.4 81P259Q (CCA→CAA) truAtRNA pseudouridine(38‑40) synthase
*NC_000913357,8750AT43.1% 61.2 / 25.4 51N272K (AAT→AAAcynRtranscriptional activator of cyn operon; autorepressor
*NC_0009133,423,2900AC43.1% 88.0 / 27.9 72intergenic (+96/+133)yhdZ/rrfFputative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon
*NC_0009131,604,2370CT43.0% 107.0 / 38.1 86S64L (TCG→TTG) lsrDautoinducer 2 import system permease protein
*NC_000913941,7130TG42.6% 97.8 / 31.4 68V252G (GTG→GGG) dmsAdimethyl sulfoxide reductase, anaerobic, subunit A
*NC_000913298,9600AC42.6% 75.6 / 25.2 55Y659D (TAT→GAT) paoCPaoABC aldehyde oxidoreductase, Moco‑containing subunit
*NC_0009134,056,1150AC42.5% 159.2 / 25.0 94G75G (GGT→GGGglnLsensory histidine kinase in two‑component regulatory system with GlnG

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 120780571 (0.860)13 (0.180) 13/266 8.9 17.1% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 63 (0.860)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 1207790 =72 (0.880)13 (0.180) 13/266 8.9 16.8% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 65 (0.890)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 925901 =61 (0.740)13 (0.160)
+C
10/296 11.6 20.0% noncoding (71/88 nt) serW tRNA‑Ser
?NC_000913 = 1097399 44 (0.540)intergenic (+570/+166) ycdU/serX putative inner membrane protein/tRNA‑Ser