breseq  version 0.27.1  revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 963 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,665,7020CA63.5% 3.2 / 108.0 107A37S (GCG→TCG) gadXacid resistance regulon transcriptional activator; autoactivator
*NC_000913993,9170AC58.3% 51.1 / 76.1 60V43G (GTG→GGG) ssuBaliphatic sulfonate ABC transporter ATPase
*NC_0009132,386,8620GA57.0% ‑4.3 / 98.0 81intergenic (+31/‑72)yfbL/yfbMputative M28A family peptidase/DUF1877 family protein
*NC_0009133,665,6980TC55.3% 17.4 / 114.6 104D38G (GAT→GGT) gadXacid resistance regulon transcriptional activator; autoactivator
*NC_0009132,332,8230AG54.7% 50.1 / 91.8 77pseudogene (1580/4605 nt)yfaSpseudogene, bacterial alpha2‑macroglobulin YfaS variant family; putative membrane protein
*NC_000913993,9130GC52.2% 66.2 / 114.4 67G44G (GGC→GGGssuBaliphatic sulfonate ABC transporter ATPase
*NC_0009131,826,3790TC51.8% 58.5 / 108.9 81E182G (GAG→GGG) astEsuccinylglutamate desuccinylase
*NC_000913357,8900CA50.0% 10.9 / 26.5 36L267F (TTG→TTTcynRtranscriptional activator of cyn operon; autorepressor
*NC_0009132,337,7230AC50.0% 103.6 / 30.2 82G566G (GGT→GGGgyrADNA gyrase (type II topoisomerase), subunit A
*NC_0009133,133,3020TG48.1% 78.1 / 35.4 82H219P (CAC→CCC) yghSputative ATP‑binding protein
*NC_0009133,443,2770AC48.0% 101.4 / 29.7 76V274G (GTA→GGA) secYpreprotein translocase membrane subunit
*NC_000913345,3280CA46.4% 125.5 / 73.8 110intergenic (+337/‑76)yahL/yahMuncharacterized protein/uncharacterized protein
*NC_0009132,378,9680CT45.8% 32.7 / 40.4 73E98K (GAA→AAA) menD2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase; SEPHCHC synthase
*NC_000913300,7110GC45.6% 111.4 / 51.0 79P75R (CCG→CGG) paoCPaoABC aldehyde oxidoreductase, Moco‑containing subunit
*NC_000913300,7210GT45.6% 108.1 / 43.9 79Q72K (CAA→AAA) paoCPaoABC aldehyde oxidoreductase, Moco‑containing subunit
*NC_0009132,826,4620GA45.1% 60.6 / 38.3 71G24E (GGA→GAA) srlEglucitol/sorbitol‑specific enzyme IIB component of PTS
*NC_000913354,2880TG44.9% 133.4 / 33.0 89V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
*NC_000913540,5580TG44.6% 109.1 / 46.0 93intergenic (+50/‑7)allB/ybbYallantoinase/putative uracil/xanthine transporter
*NC_0009132,228,2750TC44.4% 66.5 / 22.1 73pseudogene (335/1287 nt)mdtQpseudogene; putative channel/filament proteins
*NC_0009133,813,2720CT44.2% 58.6 / 42.2 87S181L (TCG→TTG) dfpfused 4'‑phosphopantothenoylcysteine decarboxylase/phosphopantothenoylcysteine synthetase, FMN‑binding

Marginal new junction evidence (lowest skew 10 of 137 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 381689NA (NA)14 (0.160) 10/278 5.9 NA noncoding (430/1331 nt) IS2 repeat region
?NC_000913 = 381743 NA (NA)noncoding (484/1331 nt) IS2 repeat region
* ? NC_000913 4409174 =NA (NA)10 (0.120) 8/266 6.5 7.5% noncoding (22/98 nt) RIP331 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP331 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
?NC_000913 4409235 = 123 (1.450)noncoding (83/98 nt) RIP331 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP331 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
* ? NC_000913 = 251823180 (0.850)8 (0.100)
+23 bp
7/250 6.5 10.6% noncoding (1/76 nt) alaW tRNA‑Ala
?NC_000913 = 3427077 NA (NA)noncoding (76/77 nt) ileU tRNA‑Ile
* ? NC_000913 225456 =11 (0.120)8 (0.100)
+23 bp
7/250 6.5 17.2% noncoding (76/77 nt) ileV tRNA‑Ile
?NC_000913 = 2518231 80 (0.850)noncoding (1/76 nt) alaW tRNA‑Ala
* ? NC_000913 = 251811774 (0.780)8 (0.100)
+22 bp
7/252 6.6 11.3% intergenic (‑1/+39) alaX/alaW tRNA‑Ala/tRNA‑Ala
?NC_000913 = 3427077 NA (NA)noncoding (76/77 nt) ileU tRNA‑Ile
* ? NC_000913 225456 =11 (0.120)8 (0.100)
+22 bp
7/252 6.6 18.1% noncoding (76/77 nt) ileV tRNA‑Ile
?NC_000913 = 2518117 74 (0.780)intergenic (‑1/+39) alaX/alaW tRNA‑Ala/tRNA‑Ala
* ? NC_000913 = 198744188 (0.930)8 (0.090) 7/276 7.1 9.0% intergenic (+13/+66) ftnB/yecJ ferritin B, putative ferrous iron reservoir/DUF2766 family protein
?NC_000913 = 1987450 79 (0.900)intergenic (+22/+57) ftnB/yecJ ferritin B, putative ferrous iron reservoir/DUF2766 family protein
* ? NC_000913 = 3427065NA (NA)8 (0.090) 7/278 7.2 NA intergenic (‑32/+11) alaU/ileU tRNA‑Ala/tRNA‑Ile
?NC_000913 = 3427068 NA (NA)intergenic (‑35/+8) alaU/ileU tRNA‑Ala/tRNA‑Ile
* ? NC_000913 225468 =14 (0.150)8 (0.090) 7/278 7.2 37.8% intergenic (+11/‑32) ileV/alaV tRNA‑Ile/tRNA‑Ala
?NC_000913 = 3427068 NA (NA)intergenic (‑35/+8) alaU/ileU tRNA‑Ala/tRNA‑Ile
* ? NC_000913 225456 =11 (0.120)8 (0.090) 7/278 7.2 43.6% noncoding (76/77 nt) ileV tRNA‑Ile
?NC_000913 = 3427056 NA (NA)intergenic (‑23/+20) alaU/ileU tRNA‑Ala/tRNA‑Ile