breseq  version 0.27.1  revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 123 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,386,8620GA71.4% 29.0 / 26.9 42intergenic (+31/‑72)yfbL/yfbMputative M28A family peptidase/DUF1877 family protein
*NC_000913328,8030TG64.1% ‑2.1 / 38.2 40T178P (ACC→CCC) betIcholine‑inducible betIBA‑betT divergent operon transcriptional repressor
*NC_000913328,8070AT62.5% ‑0.9 / 41.9 41D176E (GAT→GAAbetIcholine‑inducible betIBA‑betT divergent operon transcriptional repressor
*NC_0009131,356,2010TG61.9% 8.2 / 20.5 21T304P (ACC→CCC) sapAantimicrobial peptide transport ABC transporter periplasmic binding protein
*NC_0009133,131,4300GA60.0% 5.3 / 21.7 20L327L (CTG→TTG) yghQputative inner membrane polysaccharide flippase
*NC_0009133,131,4400TC60.0% 9.0 / 21.5 20G323G (GGA→GGGyghQputative inner membrane polysaccharide flippase
*NC_00091375,0790CT59.3% 5.2 / 30.7 27L134L (CTG→CTAthiBthiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein
*NC_0009132,903,6920CA57.5% 27.6 / 59.7 40intergenic (+318/+1055)ygcE/queEputative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
*NC_000913328,7910AG57.1% 11.1 / 38.6 42S182P (TCC→CCC) betIcholine‑inducible betIBA‑betT divergent operon transcriptional repressor
*NC_0009132,184,6460TC56.5% 13.0 / 27.7 23E219G (GAA→GGA) thiMhydoxyethylthiazole kinase
*NC_0009131,413,7350AT56.0% 12.9 / 34.4 25Y2* (TAT→TAAydaCDUF1187 family protein, Rac prophage; putative double‑strand break reduction protein
*NC_0009131,079,4680AG55.9% 20.5 / 40.5 34D55G (GAT→GGT) putPproline:sodium symporter
*NC_0009133,131,4230GC55.6% 15.1 / 26.0 18A329G (GCG→GGG) yghQputative inner membrane polysaccharide flippase
*NC_000913987,4940TG54.5% 17.2 / 30.1 22intergenic (‑512/+91)ompF/asnSouter membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
*NC_0009133,578,2870TC54.4% 37.0 / 57.8 47E147G (GAA→GGA) gntRd‑gluconate inducible gluconate regulon transcriptional repressor
*NC_00091375,0910GT53.8% 12.4 / 32.6 26D130E (GAC→GAAthiBthiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein
*NC_000913993,9040AC53.8% 29.7 / 35.5 26G47G (GGT→GGGssuBaliphatic sulfonate ABC transporter ATPase
*NC_0009133,578,2900AC53.5% 35.3 / 62.3 45V146G (GTG→GGG) gntRd‑gluconate inducible gluconate regulon transcriptional repressor
*NC_0009131,004,6250TG53.3% 36.8 / 45.0 30L227V (TTG→GTG) ycbFputative periplasmic pilin chaperone
*NC_0009133,578,3010AC53.1% 48.2 / 63.9 49G142G (GGT→GGGgntRd‑gluconate inducible gluconate regulon transcriptional repressor

Marginal new junction evidence (lowest skew 10 of 108 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 352940543 (1.060)9 (0.240) 9/278 1.9 17.5% coding (58/402 nt) hslR ribosome‑associated heat shock protein Hsp15
?NC_000913 = 3529461 45 (1.180)coding (114/402 nt) hslR ribosome‑associated heat shock protein Hsp15
* ? NC_000913 3959502 =61 (1.500)6 (0.160) 7/272 2.3 9.9% intergenic (+57/+30) ilvC/ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
?NC_000913 3959529 = 54 (1.450)intergenic (+84/+3) ilvC/ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
* ? NC_000913 = 271382935 (0.860)7 (0.190) 7/276 2.3 17.2% coding (934/1335 nt) srmB ATP‑dependent RNA helicase
?NC_000913 = 2713908 35 (0.930)coding (1013/1335 nt) srmB ATP‑dependent RNA helicase
* ? NC_000913 = 364059159 (1.450)6 (0.170) 6/254 2.4 10.6% intergenic (+46/‑271) uspA/dtpB universal stress global response regulator/dipeptide and tripeptide permease B
?NC_000913 = 3640569 51 (1.470)intergenic (+24/‑293) uspA/dtpB universal stress global response regulator/dipeptide and tripeptide permease B
* ? NC_000913 4558975 =40 (0.980)6 (0.160) 6/280 2.6 12.3% coding (552/1173 nt) iadA isoaspartyl dipeptidase
?NC_000913 4559021 = 48 (1.250)coding (506/1173 nt) iadA isoaspartyl dipeptidase
* ? NC_000913 4542682 =33 (0.810)6 (0.160) 6/280 2.6 14.3% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 4542996 = 41 (1.070)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
* ? NC_000913 = 440959535 (0.860)6 (0.150) 6/284 2.6 14.6% coding (321/732 nt) rlmB 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent
?NC_000913 = 4409617 37 (0.950)coding (343/732 nt) rlmB 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent
* ? NC_000913 4255890 =35 (0.860)6 (0.160) 6/282 2.6 14.3% coding (577/2424 nt) plsB glycerol‑3‑phosphate O‑acyltransferase
?NC_000913 4256077 = 39 (1.010)coding (390/2424 nt) plsB glycerol‑3‑phosphate O‑acyltransferase
* ? NC_000913 3060398 =32 (0.790)6 (0.160) 6/282 2.6 14.8% coding (646/894 nt) argP transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
?NC_000913 3060457 = 39 (1.010)coding (705/894 nt) argP transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
* ? NC_000913 3037860 =36 (0.880)6 (0.160) 6/276 2.6 14.8% coding (247/1734 nt) recJ ssDNA exonuclease, 5' ‑‑> 3'‑specific
?NC_000913 3037943 = 36 (0.950)coding (164/1734 nt) recJ ssDNA exonuclease, 5' ‑‑> 3'‑specific