breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 264,098 G→T L50L (CTC→CTA ykfH ← uncharacterized protein
RA 275,065 G→A T18I (ACC→ATC)  insH1 ← IS5 transposase and trans‑activator
RA 482,230 G→A P725L (CCG→CTG)  acrB ← multidrug efflux system protein
RA 1,197,502 C→T G11R (GGA→AGA)  ymfD ← e14 prophage; putative SAM‑dependent methyltransferase
RA 1,544,022 1 bp→AA coding (39/1389 nt) narU ← nitrate/nitrite transporter
RA 1,726,004 G→A intergenic (‑84/‑19) ydhL ← / → nemR DUF1289 family protein/transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,106,741 A→T S162S (TCT→TCA wbbH ← O‑antigen polymerase
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,679,525 G→T A1074D (GCT→GAT)  yphG ← DUF4380 domain‑containing TPR repeat protein
RA 2,795,396 C→A Q382K (CAG→AAG)  gabP → gamma‑aminobutyrate transporter
RA 2,817,170 C→A E112* (GAA→TAA)  yqaB ← fructose‑1‑P and 6‑phosphogluconate phosphatase
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 4,110,747 G→T intergenic (+62/+784) cdh → / ← yiiQ CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein
RA 4,159,112 G→A G236E (GGG→GAG)  oxyR → oxidative and nitrosative stress transcriptional regulator
RA 4,296,297 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,604,632 C→A A186E (GCA→GAA)  bglJ → bgl operon transcriptional activator

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 3423755–3424234 3424547–3424238 5–793 22 [21] [21] 25 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913_3_pae_tpiA 4035283–4036990 4036990 1–1708 22 [21] [20] 22 [rrsA] [rrsA]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA 273955 =NA (NA)80 (1.380) 45/278 0.4 95.2% intergenic (+1/+146) ykfC/insH1 CP4‑6 prophage; conserved protein;Phage or Prophage Related/IS5 transposase and trans‑activator
?NC_000913_3_pae_tpiA 344918 = 4 (0.070)coding (743/816 nt) yahL uncharacterized protein
* ? NC_000913_3_pae_tpiA = 275149NA (NA)62 (1.070) 42/278 0.6 96.9% intergenic (‑32/‑176) insH1/mmuP IS5 transposase and trans‑activator/CP4‑6 prophage; putative S‑methylmethionine transporter
?NC_000913_3_pae_tpiA = 344921 2 (0.030)coding (746/816 nt) yahL uncharacterized protein
* ? NC_000913_3_pae_tpiA 1263650 =69 (1.190)24 (0.420) 23/272 2.4 26.9% intergenic (‑150/‑64) lolB/hemA lipoprotein localization factor/glutamyl tRNA reductase
?NC_000913_3_pae_tpiA = 1263818 63 (1.110)coding (105/1257 nt) hemA glutamyl tRNA reductase
* ? NC_000913_3_pae_tpiA = 12994980 (0.000)61 (1.080) 54/270 0.1 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_pae_tpiA 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913_3_pae_tpiA = 1397238NA (NA)64 (1.100) 50/278 0.2 100% intergenic (+146/‑127) insH1/ynaJ IS5 transposase and trans‑activator/DUF2534 family putative inner membrane protein
?NC_000913_3_pae_tpiA = 2042468 0 (0.000)coding (101/531 nt) yodB cytochrome b561 homolog
* ? NC_000913_3_pae_tpiA 1757082 =5 (0.090)49 (0.840) 45/278 0.4 90.7% coding (1385/1413 nt) pykF pyruvate kinase I
?NC_000913_3_pae_tpiA 2101749 = NA (NA)intergenic (‑5/+146) wbbL/insH1 pseudogene, lipopolysaccharide biosynthesis protein/IS5 transposase and trans‑activator
* ? NC_000913_3_pae_tpiA = 17570851 (0.020)46 (0.790) 35/278 1.0 97.9% coding (1388/1413 nt) pykF pyruvate kinase I
?NC_000913_3_pae_tpiA = 2290113 NA (NA)intergenic (‑32/+1) insH1/yejO IS5 transposase and trans‑activator/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
* ? NC_000913_3_pae_tpiA 2042464 =1 (0.020)56 (0.970) 46/276 0.4 98.3% coding (97/531 nt) yodB cytochrome b561 homolog
?NC_000913_3_pae_tpiA = 2102942 NA (NA)intergenic (‑31/+2) insH1/wbbL IS5 transposase and trans‑activator/pseudogene, lipopolysaccharide biosynthesis protein