Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F14 I0 R1 248 61.6 2601574 98.7% 2567753 146.9

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTGATCCTGACCGTCCTGTTGCTGACGCTAGGCTCCCTCGAATGGGTTCGGTCGCGGATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGTGGGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGCTCCGTGGTTTCCCATTTATACAGTCGTCATGTTCCTATTCCATCCATGGTTTGGTTGGATGACG  >  NC_002947_CJ‑RC/2017104‑2017402
                                                                                                                                                      |                                                                                                                                                    
ATTGATCCTGACCGTCCTGTTGCTGACGCTAGGCTCCCTCGAATGGGTTCGGTCGCGGATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGTGGGGGGGGC                                                                                                                                                      >  NB501016:282:H2V7GAFX2:4:11505:16357:17266/1‑151 (MQ=60)
      CCTGACCGTCCTGTTGCTGACGCTAGGCTCCCTCGAATGGGTTCGGTCGCGGATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCT                                                                                                                                               >  NB501016:282:H2V7GAFX2:2:11303:13019:6314/1‑151 (MQ=60)
      CCTGACCGTCCTGTTGCTGACGCTAGGCTCCCTCGAATGGGTTCGGTCGCGGATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGC                                                                                                                                                <  NB501016:282:H2V7GAFX2:4:11509:14507:1866/150‑1 (MQ=60)
            CGTCCTGTTGCTGACGCTAGGCTCCCTCGAATGGGTTCGGTCGCGGATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGTTCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCG                                                                                                                                         >  NB501016:282:H2V7GAFX2:4:21404:22739:7973/1‑151 (MQ=60)
                      CTGACGCTAGGCTCCCTCGAATGGGTTCGGTCGCGGATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTG                                                                                                                                >  NB501016:282:H2V7GAFX2:4:11609:7099:5305/1‑150 (MQ=60)
                                GCTCCCTCGAATGGGTTCGGTCGCGGATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAAC                                                                                                                     >  NB501016:282:H2V7GAFX2:2:21312:26557:18044/1‑151 (MQ=60)
                                 CTCCCTCGAATGGGTTCGGTCGCGGATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACG                                                                                                                    >  NB501016:282:H2V7GAFX2:4:21504:22993:13860/1‑151 (MQ=60)
                                  TCCCTCGAATGGGTTCGGTCGCGGATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGT                                                                                                                   >  NB501016:282:H2V7GAFX2:2:21105:16546:9918/1‑151 (MQ=60)
                                   CCCTCGAATGGGTTCGGTCGCGGATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACG                                                                                                                    <  NB501016:282:H2V7GAFX2:1:21309:8312:13901/149‑1 (MQ=60)
                                    CCTCGAATGGGTTCGGTCGCGGATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCC                                                                                                                 >  NB501016:282:H2V7GAFX2:4:21604:8565:3560/1‑151 (MQ=60)
                                         AATGGGTTCGGTCGCGGATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACG                                                                                                            >  NB501016:282:H2V7GAFX2:2:11102:21436:8262/1‑151 (MQ=60)
                                             GGTTCGGTCGCGTATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAG                                                                                                        <  NB501016:282:H2V7GAFX2:3:11403:26139:18571/151‑1 (MQ=60)
                                             GGTTCGGTCGCGGATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGAC                                                                                                          >  NB501016:282:H2V7GAFX2:3:11509:19603:3595/1‑149 (MQ=60)
                                              GTTCGGTCGCGGATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGT                                                                                                       >  NB501016:282:H2V7GAFX2:1:21310:22740:18987/1‑151 (MQ=60)
                                              GTTCGGTCGCGGATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGT                                                                                                       >  NB501016:282:H2V7GAFX2:1:11107:3462:1985/1‑151 (MQ=60)
                                                     GGCGGATCATGGTGCGTATAAGCAATAGGGTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCAC                                                                                                <  NB501016:282:H2V7GAFX2:2:21201:21247:4254/151‑1 (MQ=60)
                                                     CGCGGATCATGGTGCTTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCAC                                                                                                <  NB501016:282:H2V7GAFX2:1:11108:13001:5969/151‑1 (MQ=60)
                                                     CGCGGATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCAC                                                                                                <  NB501016:282:H2V7GAFX2:2:21206:17210:18102/151‑1 (MQ=60)
                                                      GCGGATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACG                                                                                               >  NB501016:282:H2V7GAFX2:1:11203:22548:11038/1‑151 (MQ=60)
                                                                               AGGTTAGCTGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTC                                                                       <  NB501016:282:H2V7GAFX2:2:21106:25755:17259/150‑1 (MQ=60)
                                                                                          gcggcTGAGCAGGGCTGTGTACCGAGCCAGCTCCAAGCGAACGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGCTCCGTGG                                                           <  NB501016:282:H2V7GAFX2:2:21208:9875:19587/146‑1 (MQ=60)
                                                                                          GCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGCTCCGTG                                                            <  NB501016:282:H2V7GAFX2:4:21607:17660:11831/150‑1 (MQ=60)
                                                                                          GCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTT                                                                                                    <  NB501016:282:H2V7GAFX2:1:11307:11089:13591/110‑1 (MQ=60)
                                                                                              TTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGCTCCGTGGTTTC                                                       >  NB501016:282:H2V7GAFX2:4:21506:21726:14013/1‑151 (MQ=60)
                                                                                               TGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGCTCCGTGGTTTC                                                       <  NB501016:282:H2V7GAFX2:1:21201:10859:8337/150‑1 (MQ=60)
                                                                                                GAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGCTCCGTGGTTTCC                                                      <  NB501016:282:H2V7GAFX2:1:21106:23242:17685/150‑1 (MQ=60)
                                                                                                               CCGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTAGACGCTCCGTGGTTTACCATTTATACACTCGT                                       >  NB501016:282:H2V7GAFX2:4:11603:20934:9449/1‑150 (MQ=60)
                                                                                                                CGAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTggccttttttggcgcggcgggggttgccatttttaaagtcgtgt                                     >  NB501016:282:H2V7GAFX2:1:11308:9053:8307/1‑107 (MQ=60)
                                                                                                                 GAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGCTCCGTGGTTTCCCATTTATACAGTCGTCAT                                    >  NB501016:282:H2V7GAFX2:4:21409:8903:14556/1‑151 (MQ=60)
                                                                                                                 GAGCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGGTGGCATTTTTAGACGCTCCGTGGTTTGCCATTTATAAAGTCGTGAT                                    >  NB501016:282:H2V7GAFX2:2:21101:7288:8071/1‑151 (MQ=60)
                                                                                                                   GCCAGCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTAGCATTTTTAGACGCGCCGTGGTTTTCCATTTATtaagtagtgcagt                                  >  NB501016:282:H2V7GAFX2:4:11602:3774:1077/1‑138 (MQ=60)
                                                                                                                       GCTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGCTCCGTGGTTTCCCATTTATACAGTCGTCATGTTCCT                              <  NB501016:282:H2V7GAFX2:4:21602:20784:18562/151‑1 (MQ=60)
                                                                                                                        CTTCAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTAGACGCTCCGTGGTTTCCCATTTATAAAGTCGTCATGTTACT                              >  NB501016:282:H2V7GAFX2:1:11212:6864:16182/1‑150 (MQ=60)
                                                                                                                           CAAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTAGCATTTTTCGACGCTCCGTGGTTTCCCATTTATACAGTCGTCATGTTggtattt                          >  NB501016:282:H2V7GAFX2:2:11307:15579:5587/1‑144 (MQ=60)
                                                                                                                            AAGCGATCGCTTCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGGATTTTTCGACGCGCCGTGGTTTCCCATTTttaaagtcgtgattttggttata                          >  NB501016:282:H2V7GAFX2:2:11109:16042:11450/1‑127 (MQ=60)
                                                                                                                                       TCAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGCTCCGTGGTTTCCCATTTATACAGTCGTCATGTTCCTATTCCATCCATGGTT               <  NB501016:282:H2V7GAFX2:3:21407:13308:14372/150‑1 (MQ=60)
                                                                                                                                        CAGAGT‑GGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGCTCCGTGGTTTCCCATTTATACAGTCGTCATGTTCCTATTCCATCCATGGTTTG             <  NB501016:282:H2V7GAFX2:4:21506:17544:10997/151‑1 (MQ=60)
                                                                                                                                                 GGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGCTCCGTGGTTTCCCATTTATACAGTCGTCATGTTCCTATTCCATCCATGGTTTGGTTGGATG     <  NB501016:282:H2V7GAFX2:2:11112:25621:11521/151‑1 (MQ=60)
                                                                                                                                                    GGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGCCGCTCCGTGGTTTCCCATTTTTACAGTCGTCATGTTGCTATTTCCTACATGGTTTGGTTGGATGACG  >  NB501016:282:H2V7GAFX2:3:11610:3729:17837/1‑151 (MQ=60)
                                                                                                                                                      |                                                                                                                                                    
ATTGATCCTGACCGTCCTGTTGCTGACGCTAGGCTCCCTCGAATGGGTTCGGTCGCGGATCATGGTGCGTATAAGCAATAGGTTAGATGTGCTGTTGAGCAGGGATGTGTACCGAGCCAGCTTCAAGCGATCGCTTCAGAGTGGGGGGGGGCGATGCTACGGCGCAGTCGTTGAATGACCTAACGTCCTTACGACAGTTTTTCACGGGAGCGGGCGTGTTCGCATTTTTCGACGCTCCGTGGTTTCCCATTTATACAGTCGTCATGTTCCTATTCCATCCATGGTTTGGTTGGATGACG  >  NC_002947_CJ‑RC/2017104‑2017402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑