Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F121 I0 R1	43	32.8	695000	96.9%	673455	140.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,481,355	G→T	100%	A6890E (GCA→GAA)	ebh ←	extracellular matrix binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,481,355	0	G	T	100.0%	85.9 / NA	30	A6890E (GCA→GAA)	ebh	extracellular matrix binding protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (12/18); total (12/18)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTGCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTT  >  CP000730/1481229‑1481483

ttCAGTTCCTGTGCTGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTTATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTCAGTTAGCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCa                                                                                                                    <  1:150648/141‑1 (MQ=255)

ttCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCa                                                                                                                    >  2:267232/1‑141 (MQ=255)

ttCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCa                                                                                                                    <  1:50594/141‑1 (MQ=255)

   aGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCt                                                                                                                 <  1:171750/141‑1 (MQ=255)

           aGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTGCttt                                                                                                         >  2:16186/1‑141 (MQ=255)

              ggTTGGAGTGTCTTGCGTTGCTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCt                                                                                                      <  2:16212/141‑1 (MQ=255)

                       ggCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCAtt                                                                                             <  1:53066/141‑1 (MQ=255)

                         cTTGCGTTAGTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCa                                                                                           >  1:252908/1‑141 (MQ=255)

                              gTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCt                                                                                      <  1:221636/141‑1 (MQ=255)

                                  cTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGtt                                                                                  >  2:203865/1‑141 (MQ=255)

                                         tACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAgt                                                                           >  1:35766/1‑141 (MQ=255)

                                         tACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAgt                                                                           >  2:234791/1‑141 (MQ=255)

                                                 gggcTTGATTGATGTTTCCAGTAAACGTACCTTTTTGTGCATTGTTTAAGTTTTCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAg                                                                   <  2:139924/139‑1 (MQ=255)

                                                 gcgcTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAg                                                                   >  2:315504/1‑141 (MQ=255)

                                                       gattgatGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGaaa                                                             <  1:298330/141‑1 (MQ=255)

                                                              gTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTCAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGtgctgc                                                      >  1:121993/1‑141 (MQ=255)

                                                                  cctgtacctgCACCTTTTTGTGCTTTGTTTAAGCTATCTAACGTATATTGTGTTTGGTTTTCGTTGTTTTTAGCATCTGTTACTTTTCTATTCCCATGCAACGCTTGTTTCGGAGTTGTTACAGTTGAAAGTGCTGCTTCa                                                  <  1:233671/132‑1 (MQ=255)

                                                                  ccAGTAACGGCACCGTGTTGTGCATTGTTTAAGTTACCTAACGTAGATAATGTTTGGTTTTCAGTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCa                                                  <  1:206723/141‑1 (MQ=255)

                                                                        aCAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTtgg                                            >  1:6917/1‑140 (MQ=255)

                                                                              ccTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTgg                                      <  1:196572/141‑1 (MQ=255)

                                                                                         aTTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGt                           <  2:251984/141‑1 (MQ=255)

                                                                                          ttGTTTAAGTTCTCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTa                          <  2:282196/141‑1 (MQ=255)

                                                                                             tttAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATATGTTACTTGTCTGTCTCCTTGCAATGCTTGTTTCGTAGTTGTTACAGTGGAAATTGCTGCTTCAAGTTTTGTTTGGTTCGCGTTTTTAccg                       >  2:189042/1‑140 (MQ=255)

                                                                                               tAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCAtt                     <  1:66203/141‑1 (MQ=255)

                                                                                                   ttATCTAACGTAGATATTGTTTGGTTTTCATTCTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTTTTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCt                 <  2:67230/141‑1 (MQ=255)

                                                                                                        tacgttAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCACCTTGTGATTGGTTCGCATTTGTACCATTTGCTTGAtt            >  1:119070/6‑141 (MQ=255)

                                                                                                          aCGTAGATAATGTTTGGTTTTCATTGTTTTTTGCATCTGTTTCTTTTCTATTGCCATTCAACGCTTTTTTCGTAGTTGTTACAGTTGAAAGTGCTTGTTCAATTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATtaa          <  2:61476/141‑1 (MQ=255)

                                                                                                          aCGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATtaa          >  1:171293/1‑141 (MQ=255)

                                                                                                            gtgggtAATGTTGTGTTTTCAGTGTTTGGAGCAGCTGTTACTTTTGTATCAGCATTGAACGCTTGTTTCGTAGTTGTGACAGTTGAAAGTGCTGCTTCAACGTGTGGGTGGTTCGCAGTGTTACCGTTTGGGGGATtaata        <  2:333961/136‑1 (MQ=255)

                                                                                                                  aaTGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATAttt  <  1:300712/141‑1 (MQ=255)

TTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTGCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTT  >  CP000730/1481229‑1481483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A