Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F67 I1 R1	27	23.6	499472	97.1%	484987	140.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	629,681	C→A	L84I (CTA→ATA)	hxlA →	possible 3‑hexulose‑6‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	629,681	0	C	A	100.0%	59.2 / NA	19	L84I (CTA→ATA)	hxlA	possible 3‑hexulose‑6‑phosphate synthase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (8/11); total (8/11)

CGAAGGTTTACCAGCAGTTAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGGGTGCTGAT  >  CP000730/629545‑629818

cGAAGGTTCACCAGCAGTAAAACAAACGGAAGACAACACGAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGAATATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAgg                                                                                                                                       <  1:90684/141‑1 (MQ=255)

                                    caTTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTga                                                                                                   <  1:43144/141‑1 (MQ=255)

                                           aaTGTAAAAGTATTAGCAGAAATGAAAATTATAGATGCAGCTGTTTATGAAGTTAGCCAAGAAATTAAATTTGGCGCGGATGTAATTATAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGTTGAAGcg                                                                                            >  1:50385/1‑140 (MQ=255)

                                                 aaaGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATaaa                                                                                      >  1:63589/1‑141 (MQ=255)

                                                 aaaGTATCAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATaaa                                                                                      <  1:63949/141‑1 (MQ=255)

                                                   aGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATaaaaa                                                                                    >  1:103194/1‑141 (MQ=255)

                                                   aGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATaaaaa                                                                                    >  1:107035/1‑141 (MQ=255)

                                                   aGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGAAGTTAAAGTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATaaaaa                                                                                    >  1:413636/1‑141 (MQ=255)

                                                       ttAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGGGTTGCAGAAGAGGCATCAATTCAAGCAGCTATTGAAGAAGCTCATAAAaataat                                                                                >  1:12864/1‑141 (MQ=255)

                                                         agcataaaagAAAAATATAGATTCAGTCGATTATGAAGTTAGACAAGGAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAAtaataa                                                                              <  1:30914/132‑1 (MQ=255)

                                                         aGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGAAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAAtaataa                                                                              <  1:129047/141‑1 (MQ=255)

                                                                   aaaaTTATAGATGCAGCTGAATATGAAGTTAGCCAAGCAATTAAGTTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCACCAATTAAAGCAGCTACTGAAGAAACTCATAAAAATAATAAACAATTACTa                                                                    >  1:301161/1‑141 (MQ=255)

                                                                         ataGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGat                                                              <  1:17465/141‑1 (MQ=255)

                                                                            gATGCAGATGATTATGAAGTTAGACAAGCAATTAAATTTGGCGAGGATGTAATTACAATAATAGGTGTTAGAGAAGATGCATCATATGCATCTGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATg                                                           <  1:363601/141‑1 (MQ=255)

                                                                                 aGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGc                                                      <  1:494645/141‑1 (MQ=255)

                                                                                 aGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGc                                                      <  1:14922/141‑1 (MQ=255)

                                                                                  gCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCt                                                     <  1:43498/141‑1 (MQ=255)

                                                                                   cTGATTATGAAGTTAGACAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTg                                                    <  1:310051/141‑1 (MQ=255)

                                                                                                                   ggCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTa                    >  1:438770/1‑141 (MQ=255)

                                                                                                                                     ataataGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGGGTGCTGAt  <  1:324510/141‑1 (MQ=255)

CGAAGGTTTACCAGCAGTTAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGGGTGCTGAT  >  CP000730/629545‑629818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A