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Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A69 F1 I1 R1	20	59.3	3764421	95.9%	3610079	75.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	REL606	2,100,308	Δ22,146 bp		ogrK–[ECB_02013]	27 genes ogrK, yegZ, ECB_01989, ECB_01990, ECB_01991, ECB_01992, ECB_01993, ECB_01994, ECB_01995, ECB_01996, ECB_01997, ECB_01998, ECB_01999, ECB_02000, ECB_02001, ECB_02002, ECB_02003, ECB_02004, ECB_02005, ECB_02006, ECB_02007, ECB_02008, ECB_02009, ECB_02010, ECB_02011, ECB_02012, [ECB_02013]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	2100308	2122453	22146	76 [1]	[4] 76	ogrK–[ECB_02013]	ogrK,yegZ,ECB_01989,ECB_01990,ECB_01991,ECB_01992,ECB_01993,ECB_01994,ECB_01995,ECB_01996,ECB_01997,ECB_01998,ECB_01999,ECB_02000,ECB_02001,ECB_02002,ECB_02003,ECB_02004,ECB_02005,ECB_02006,ECB_02007,ECB_02008,ECB_02009,ECB_02010,ECB_02011,ECB_02012,[ECB_02013]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 2100307	1 (0.020)	62 (1.530)	43/104	0.0	96.4%	intergenic (+171/+102)	yegQ/ogrK	predicted peptidase/phage DNA‑binding transcriptional regulator
*	?	REL606	2122454 =	4 (0.100)	62 (1.530)	43/104	0.0	96.4%	coding (172/216 nt)	ECB_02013	conserved hypothetical protein; putative exported protein

GATK/CNVnator alignment

N/A