Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A46 F1 I1 R1	17	71.0	4433184	98.0%	4344520	76.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	REL606	3,912,149	Δ15 bp	coding (28‑42/99 nt)	ilvL →	ilvG operon leader peptide

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 3912148	1 (0.010)	60 (0.960)	44/132	0.3	98.5%	coding (27/99 nt)	ilvL	ilvG operon leader peptide
*	?	REL606	3912164 =	1 (0.020)	60 (0.960)	44/132	0.3	98.5%	coding (43/99 nt)	ilvL	ilvG operon leader peptide

CAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/3912065‑3912148

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtgattagcGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAA  >  REL606/3912164‑3912238

CAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAATAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAG                                                                                     >  1:2018049/1‑76

CAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAG                                                                                     >  2:1633130/1‑76

CAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAG                                                                                     <  2:2154499/76‑1

 AAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGT                                                                                    >  1:465092/1‑76

 AAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGT                                                                                    >  1:596448/1‑76

 AAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGT                                                                                    >  1:799470/1‑76

  AAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTG                                                                                   <  1:299230/76‑1

  AAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTG                                                                                   >  2:1817151/1‑76

     CCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATT                                                                                >  1:1367604/1‑76

     CCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATT                                                                                <  1:769871/76‑1

     CCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATT                                                                                <  1:825103/76‑1

      CTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTA                                                                               <  2:1578078/76‑1

      CTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTA                                                                               <  1:2056868/76‑1

      CTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTA                                                                               >  1:747557/1‑76

      CTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTA                                                                               >  2:1724158/1‑76

        ATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGC                                                                             >  1:1878633/1‑76

        ATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGC                                                                             >  2:1018658/1‑76

         TGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCG                                                                            <  1:69621/76‑1

           GTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTG                                                                          >  1:936462/1‑76

                TCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACCGCCCTTCTACGAGTGATTAGCGTGGTGGT                                                                     >  2:368496/1‑76

                TCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGT                                                                     >  2:368519/1‑76

                 CTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTG                                                                    <  1:1445114/76‑1

                  TTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGA                                                                   >  1:353748/1‑76

                   TTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGAT                                                                  >  1:1613791/1‑76

                    TAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATT                                                                 >  1:861923/1‑76

                    TAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATT                                                                 >  2:2196312/1‑76

                     AGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTA                                                                >  1:1472279/1‑76

                       GCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATT                                                              >  2:2157401/1‑76

                       GCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATT                                                              <  2:848138/76‑1

                       GCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATT                                                              <  2:848109/76‑1

                         ATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTAT                                                            >  1:29274/1‑76

                         ATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTAT                                                            >  1:884623/1‑76

                         ATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTAT                                                            <  2:1903492/76‑1

                              TTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCAC                                                       <  2:740915/76‑1

                               TCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACC                                                      >  1:1857717/1‑76

                                 GAACAAGATGCAAGAAAAGACATAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGT                                                    <  1:352478/76‑1

                                  AACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTG                                                   >  1:301585/1‑76

                                  AACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTG                                                   >  1:2154712/1‑76

                                   ACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGC                                                  >  1:362335/1‑76

                                    CAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCG                                                 >  2:468731/1‑76

                                     AAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGG                                                >  2:737454/1‑76

                                     AAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGG                                                <  1:1448339/76‑1

                                      AGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGG                                               >  2:21868/1‑76

                                        ATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGC                                             >  2:2031337/1‑76

                                         TGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCT                                            >  2:2050944/1‑76

                                           CAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGC                                          >  1:1728814/1‑76

                                           CAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGC                                          >  1:1110067/1‑76

                                            AAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCA                                         >  2:75029/1‑76

                                            AAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCA                                         >  2:825157/1‑76

                                                     CAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGA                                <  2:1988267/76‑1

                                                     CAAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGA                                >  2:2184062/1‑76

                                                      AAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCNTGCGGGGCTGCACTTGGACGAG                               <  2:300126/76‑1

                                                      AAAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAG                               >  2:1077573/1‑76

                                                       AAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGG                              <  1:1931404/76‑1

                                                       AAATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGG                              <  1:11695/76‑1

                                                         ATGACAGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAA                            <  2:973124/76‑1

                                                              AGCCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCT                       >  2:965332/1‑76

                                                                CCCTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTA                     <  2:465092/76‑1

                                                                 CCTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAG                    <  1:602526/76‑1

                                                                  CTTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGA                   >  1:1756196/1‑76

                                                                   TTCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAG                  >  1:170801/1‑76

                                                                    TCTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGA                 <  2:387984/76‑1

                                                                     CTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGAT                <  2:1596710/76‑1

                                                                     CTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGAT                >  1:602444/1‑76

                                                                     CTACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGAT                <  2:2114010/76‑1

                                                                      TACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATC               <  1:533380/76‑1

                                                                       ACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCA              <  1:847073/76‑1

                                                                       ACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCA              >  2:243041/1‑76

                                                                       ACGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCA              <  1:1589715/76‑1

                                                                        CGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAA             <  1:1077573/76‑1

                                                                        CGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAA             <  1:1295008/76‑1

                                                                        CGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAA             <  1:627801/76‑1

                                                                        CGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAA             <  2:938443/76‑1

                                                                        CGAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAA             <  2:1024981/76‑1

                                                                         GAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAG            <  1:820674/76‑1

                                                                         GAGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAG            <  1:820701/76‑1

                                                                         GAGGGATTGGCGTGG‑GGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGC           <  2:155516/76‑1

                                                                           GTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGTACGAGGAAAGGCTTAGAGATCAAGCC          <  2:1268059/76‑1

                                                                            TGATTAGCGTGGTGGTGATTATTAGCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCT         <  1:2199610/76‑1

                                                                             GATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTT        >  1:2039916/1‑76

                                                                             GATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTT        >  1:1335754/1‑76

                                                                             GATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTT        >  1:775256/1‑76

                                                                              ATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTA       <  1:1894770/76‑1

                                                                              ATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTA       <  1:1042970/76‑1

                                                                               TTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAA      <  2:1756196/76‑1

                                                                                TAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAAC     >  1:918201/1‑76

                                                                                 AGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACG    <  2:58585/76‑1

                                                                                 AGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACG    <  2:702737/76‑1

                                                                                 AGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACG    <  1:782505/76‑1

                                                                                 AGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACG    <  2:861923/76‑1

                                                                                   CGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAA  <  1:1719411/76‑1

CAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/3912065‑3912148

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtgattagcGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAA  >  REL606/3912164‑3912238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.

GATK/CNVnator alignment

N/A