Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A22 F999 I0 R1	108	38.1	2810592	98.4%	2765622	49.6

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,980,957	(G)_6→7	coding (71/1386 nt)	yifK →	putative transporter YifK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,980,951	1	.	G	96.0%	90.1 / ‑3.0	25	coding (65/1386 nt)	yifK	putative transporter YifK
Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (15/9); total (16/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑GG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTA  >  NC_000913/3980902‑3980995

ccAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCt                                                <  1:1660843/50‑1 (MQ=255)

  agagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg                                             >  1:1807460/1‑50 (MQ=255)

    agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg‑gg                                          >  1:1355648/1‑50 (MQ=255)

      cTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg‑gggg                                        >  1:2666430/1‑50 (MQ=255)

       tACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg‑ggggg                                       >  1:236024/1‑49 (MQ=255)

         cAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg‑gggggc                                      <  1:1292670/49‑3 (MQ=255)

              tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCAtt                                 <  1:1135881/50‑1 (MQ=255)

                ggCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTgg                               <  1:1807474/50‑1 (MQ=255)

                  cTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCg                             >  1:1994787/1‑50 (MQ=255)

                     gAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGt                            <  1:798727/48‑1 (MQ=255)

                      aaGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCgg                         >  1:159651/1‑50 (MQ=255)

                       aGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGc                        >  1:154381/1‑50 (MQ=255)

                           cGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGt                    >  1:292145/1‑50 (MQ=255)

                             aCATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGttt                  <  1:177565/50‑1 (MQ=255)

                             aCATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGtt                   >  1:19641/1‑49 (MQ=39)

                               atatCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTAt                >  1:1891466/1‑50 (MQ=255)

                                tatCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATg               >  1:362523/1‑50 (MQ=255)

                                tatCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATg               >  1:2347780/1‑50 (MQ=255)

                                   cGAACTCATCGCCCT‑GGTGGGGCACCATTGGCGTCGGCCTGTTTATgggg            >  1:683354/1‑50 (MQ=39)

                                    gAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATggggg           >  1:1222781/1‑50 (MQ=255)

                                    gAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATgggg            <  1:859963/49‑1 (MQ=39)

                                      aCTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGgcc         <  1:2041844/50‑1 (MQ=255)

                                       cTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccg        >  1:2075815/1‑50 (MQ=255)

                                       cTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccg        <  1:2729306/50‑1 (MQ=255)

                                       cTCATCGCCCTGGG‑GGGGCACCATTGGCGTCAGCCTGTTTATGGGGgccg        >  1:1249424/1‑50 (MQ=255)

                                        tCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccgc       >  1:2052664/1‑50 (MQ=255)

                                         cATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccgc       >  1:2572790/1‑49 (MQ=39)

                                          aTCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccgc       >  1:2778904/1‑48 (MQ=39)

                                             gCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGt   <  1:2337591/49‑1 (MQ=39)

                                             gCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGt   <  1:175425/49‑1 (MQ=39)

                                             gCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTa  >  1:1239036/1‑50 (MQ=255)

CCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑GG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTA  >  NC_000913/3980902‑3980995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

N/A