Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F17 I0 R1
|
87 |
56.5 |
4283937 |
98.6% |
4223961 |
62.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,606,089 |
G→A |
11.1% |
N37N (AAC→AAT) |
dnaT ← |
DNA biosynthesis protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,606,089 | 0 | G | A | 11.1%
| 104.9
/ 4.3
| 36 | N37N (AAC→AAT) | dnaT | DNA biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base G (16/16); new base A (2/2); total (18/18) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 |
CAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGT > W3110S.gb/4606020‑4606159
|
cAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGt > 1:400493/1‑71 (MQ=255)
gCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGt > 1:1192470/1‑69 (MQ=255)
cTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAg < 1:3853291/71‑1 (MQ=255)
ttcttcCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCa < 1:258132/70‑1 (MQ=255)
cttcCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCaaa > 1:1008997/1‑70 (MQ=255)
cttcCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCaaa < 1:2405973/70‑1 (MQ=255)
cttcCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCaaa < 1:1831399/70‑1 (MQ=255)
ttcCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATa > 1:3573059/1‑71 (MQ=255)
ccAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATAc < 1:1175/70‑1 (MQ=255)
ccAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGCAAATa < 1:3500254/69‑1 (MQ=255)
aGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGCAAATACgg > 1:4216027/1‑70 (MQ=255)
gcCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATAAGGCAAc > 1:736823/1‑71 (MQ=255)
gcagTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACcac > 1:2221297/1‑70 (MQ=255)
ttCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGc > 1:1360055/1‑71 (MQ=255)
ttCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGc > 1:3800848/1‑71 (MQ=255)
cAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCt > 1:2041540/1‑71 (MQ=255)
ccAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGc < 1:2499382/46‑1 (MQ=255)
gcgtgcaggcgtgACGGCATAAAACGCCGGGGCATTGt < 1:2674885/38‑1 (MQ=255)
gcgtgcaggcgtgACGGCATAAAACGCCGGGGCATTGTTAGCaa > 1:1554529/1‑44 (MQ=255)
gcgtgcaggcgtgACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCtt < 1:1427137/71‑1 (MQ=255)
caggcgtgACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGc < 1:546833/70‑1 (MQ=255)
caggcgtgACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGc > 1:3334831/1‑70 (MQ=255)
aggcgtgACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCa < 1:1786384/71‑1 (MQ=255)
gcgtgACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAg > 1:179076/1‑70 (MQ=255)
gtgACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAAc > 1:2263516/1‑71 (MQ=255)
aCGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTc > 1:2416060/1‑53 (MQ=255)
tAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTg < 1:4132202/71‑1 (MQ=255)
aaaaCGCCGGGGCATTATTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGa > 1:2753500/1‑71 (MQ=255)
aaaCGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGAt > 1:1490236/1‑71 (MQ=255)
aaaCGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGAt > 1:3463273/1‑71 (MQ=255)
aCGCCTGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCg < 1:418877/71‑1 (MQ=255)
aCGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAAGGGTTTGGTGATCg < 1:4276757/71‑1 (MQ=255)
ggggCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTTATCGTGTAc < 1:1872339/71‑1 (MQ=255)
gggCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTAcc < 1:3363629/71‑1 (MQ=255)
ttGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTAcc < 1:1098233/66‑1 (MQ=255)
ttGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAgg < 1:590115/69‑1 (MQ=255)
gTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGt > 1:1208742/1‑71 (MQ=255)
gTTAGCAAATACGGCAACCACACCGCC‑TCAGCTTTTGCCAGAACGGTTTGGTGATCGTCTa > 1:498581/1‑61 (MQ=255)
|
CAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGT > W3110S.gb/4606020‑4606159
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
