Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,606,089 |
G→A |
N37N (AAC→AAT) |
dnaT ← |
DNA biosynthesis protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,606,089 | 0 | G | A | 100.0%
| 76.7
/ NA
| 25 | N37N (AAC→AAT) | dnaT | DNA biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/3); total (22/3) |
CCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGT > W3110S.gb/4606049‑4606141
|
ccAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACcac > 1:713215/1‑61 (MQ=255)
ccAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACcac > 1:1616761/1‑61 (MQ=255)
ccAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACcac > 1:4689873/1‑61 (MQ=255)
ccAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACcac > 1:1754160/1‑61 (MQ=255)
ccAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACcac > 1:2243757/1‑61 (MQ=255)
ccAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACcac > 1:2388459/1‑61 (MQ=255)
ccAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACcac > 1:2464490/1‑61 (MQ=255)
ccAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACcac > 1:2621894/1‑61 (MQ=255)
ccAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACcac > 1:3783471/1‑61 (MQ=255)
ccAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACcac > 1:3653684/1‑61 (MQ=255)
gcgtgcaggcgtgACGGCATAAAACGCCGGGGCATTATTAGCaaa > 1:4430784/1‑45 (MQ=255)
gcgtgcaggcgtgACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGc > 1:3958802/1‑62 (MQ=255)
gcgtgcaggcgtgACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGc > 1:2215748/1‑62 (MQ=255)
gcgtgcaggcgtgACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGc > 1:2669748/1‑62 (MQ=255)
gcgtgcaggcgtgACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGc > 1:2702128/1‑62 (MQ=255)
gtgACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGcc < 1:851943/53‑1 (MQ=255)
ggCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGcc < 1:1581000/61‑1 (MQ=255)
cATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGa > 1:2944698/1‑62 (MQ=255)
cATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGa > 1:2063824/1‑62 (MQ=255)
cATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGa > 1:4138588/1‑62 (MQ=255)
cATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGa > 1:1916613/1‑62 (MQ=255)
cATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGa > 1:447078/1‑62 (MQ=255)
cATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGa > 1:47055/1‑62 (MQ=255)
gCCGGGGCATTATTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTg < 1:1715875/62‑1 (MQ=255)
ggggCATTATTAGCAAATACGGCAACCACACCGCCATCAGCTTTTGCCAGAGCGGTTTGGt > 1:1123577/1‑61 (MQ=255)
|
CCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGT > W3110S.gb/4606049‑4606141
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
