Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F21 I0 R2 316 65.6 5147759 97.4% 5013917 60.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 556,858 A→T 100% L36Q (CTG→CAG)  folD ← bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb556,8580AT100.0% 96.9 / NA 33L36Q (CTG→CAG) folDbifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (15/18);  total (15/18)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCAC  >  W3110S.gb/556790‑556922
                                                                    |                                                                
cacaAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGt                                                                >  1:195242/1‑71 (MQ=255)
   aaGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTcc                                                              <  1:3565564/70‑1 (MQ=255)
   aaGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCt                                                             >  1:4577685/1‑71 (MQ=255)
   aaGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCt                                                             <  1:3157925/71‑1 (MQ=255)
    aGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTg                                                            <  1:4967385/71‑1 (MQ=255)
    aGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTg                                                            <  1:3169342/71‑1 (MQ=255)
              ttgcttgcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCa                                                   <  1:4245084/70‑1 (MQ=255)
                gcttgcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGt                                                 <  1:1178871/70‑1 (MQ=255)
                    gcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTcc                                               <  1:869741/68‑1 (MQ=255)
                         aTAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaa                                       <  1:1664928/71‑1 (MQ=255)
                         aTAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaa                                       <  1:3160410/71‑1 (MQ=255)
                          tAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAg                                                  <  1:3445801/59‑1 (MQ=255)
                          tAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCATTCCGGCTGCaa                                       >  1:2579775/1‑70 (MQ=255)
                          tAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAAt                                      <  1:2044550/71‑1 (MQ=255)
                               ttGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAcgc                                  >  1:3809429/1‑70 (MQ=255)
                                tGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATa                                     >  1:2619707/1‑66 (MQ=255)
                                     tGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGaa                           >  1:1013590/1‑71 (MQ=255)
                                     tGCAGGGTTACTACACACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGaa                           >  1:1373126/1‑71 (MQ=255)
                                       cAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAAc                          <  1:2112145/70‑1 (MQ=255)
                                        aGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACtt                        <  1:2129980/71‑1 (MQ=255)
                                         gggTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttt                       <  1:2144929/71‑1 (MQ=255)
                                           gTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttttt                     >  1:504156/1‑71 (MQ=255)
                                            ttACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGcga                                       >  1:3619754/1‑50 (MQ=255)
                                                  cccACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAAc              >  1:2951248/1‑71 (MQ=255)
                                                    caccaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACtt            >  1:2054336/1‑71 (MQ=255)
                                                     accaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCa                <  1:3099132/66‑1 (MQ=255)
                                                      ccaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCa                <  1:3037974/65‑1 (MQ=255)
                                                          cacaACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCa          >  1:1817999/1‑67 (MQ=255)
                                                          cacaACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAgc      >  1:1558880/1‑71 (MQ=255)
                                                            caACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAgcgc    <  1:2789467/71‑1 (MQ=255)
                                                            caACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAgcgc    <  1:4347176/71‑1 (MQ=255)
                                                              aCGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCAc  >  1:2868823/1‑71 (MQ=255)
                                                              aCGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCAc  >  1:4718701/1‑71 (MQ=255)
                                                                    |                                                                
CACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCAC  >  W3110S.gb/556790‑556922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A