Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F8 I0 R1
|
43 |
62.4 |
4721813 |
98.4% |
4646263 |
62.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
556,858 |
A→T |
100% |
L36Q (CTG→CAG) |
folD ← |
bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 556,858 | 0 | A | T | 100.0%
| 123.5
/ NA
| 42 | L36Q (CTG→CAG) | folD | bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (19/23); total (19/23) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACC > W3110S.gb/556790‑556923
|
cacaAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGt > 1:4303259/1‑71 (MQ=255)
caAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTAGTACCCACCAGCACAACGGCCTGTcc > 1:1446277/1‑71 (MQ=255)
caAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTcc < 1:513162/71‑1 (MQ=255)
caAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTcc < 1:2757781/71‑1 (MQ=255)
aGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTg < 1:240546/71‑1 (MQ=255)
gCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTcc < 1:4246107/63‑1 (MQ=255)
gCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTcc < 1:1927351/63‑1 (MQ=255)
cGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCg < 1:1170792/71‑1 (MQ=255)
gTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCg > 1:1132461/1‑70 (MQ=255)
tttGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTcc < 1:4295924/60‑1 (MQ=255)
tttGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTcc < 1:3753185/60‑1 (MQ=255)
tttGCTTGCGACATAAATTTGCGATCCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCa < 1:3147308/71‑1 (MQ=255)
tgcttgcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGt > 1:854353/1‑71 (MQ=255)
ttgcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCt < 1:2610477/56‑1 (MQ=255)
gcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTgg > 1:4631667/1‑56 (MQ=255)
gcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCgg < 1:2203088/70‑1 (MQ=255)
gcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGc > 1:2412718/1‑71 (MQ=255)
aCATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc < 1:3072522/71‑1 (MQ=255)
cATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc > 1:234053/1‑70 (MQ=255)
aTAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTg < 1:1275943/50‑1 (MQ=255)
aTAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaa < 1:2305519/71‑1 (MQ=255)
aaaTTTGCGATGCATGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATa > 1:3690934/1‑71 (MQ=255)
aaaTTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGt < 1:22627/44‑1 (MQ=255)
tttGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAcgc < 1:3735782/71‑1 (MQ=255)
tGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACgcgc > 1:2324632/1‑71 (MQ=255)
aGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACtt < 1:1206503/71‑1 (MQ=255)
gggTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttt < 1:4016842/71‑1 (MQ=255)
ggTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATa > 1:1042736/1‑56 (MQ=255)
gTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttttt < 1:1722570/71‑1 (MQ=255)
gTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttttt > 1:1630720/1‑71 (MQ=255)
gTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttttt < 1:438645/71‑1 (MQ=255)
ttACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCgg > 1:2810348/1‑46 (MQ=255)
ccaccaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACt < 1:3565894/71‑1 (MQ=255)
caccaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACtt < 1:3052522/71‑1 (MQ=255)
ccaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACtttt < 1:4647163/59‑1 (MQ=255)
aGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCa > 1:627340/1‑69 (MQ=255)
cacaACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAg > 1:3880659/1‑59 (MQ=255)
caACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAgcg > 1:2057170/1‑70 (MQ=255)
caACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAgcg > 1:4249319/1‑70 (MQ=255)
aaCGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaaa > 1:3933453/1‑35 (MQ=39)
aaCGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCa > 1:3684223/1‑71 (MQ=255)
ggCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCAcc > 1:4614953/1‑70 (MQ=255)
|
CACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACC > W3110S.gb/556790‑556923
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
