Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I0 R2
|
201 |
99.6 |
7939938 |
96.9% |
7693799 |
60.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
556,858 |
A→T |
100% |
L36Q (CTG→CAG) |
folD ← |
bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 556,858 | 0 | A | T | 100.0%
| 189.9
/ NA
| 64 | L36Q (CTG→CAG) | folD | bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (24/40); total (24/40) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTG > W3110S.gb/556792‑556925
|
caAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTcc < 1:4814602/71‑1 (MQ=255)
caAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTcc < 1:605506/71‑1 (MQ=255)
caAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTcc < 1:6107873/71‑1 (MQ=255)
gCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTgg < 1:4238829/71‑1 (MQ=255)
gCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTgg < 1:1421963/71‑1 (MQ=255)
ccTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGt < 1:3198156/71‑1 (MQ=255)
cTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTg < 1:2724646/71‑1 (MQ=255)
gCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGc < 1:4300695/69‑1 (MQ=255)
cGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCg > 1:1954094/1‑71 (MQ=255)
gTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTc > 1:7901908/1‑60 (MQ=255)
gTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGccc > 1:1254689/1‑69 (MQ=255)
gTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGc < 1:4895937/71‑1 (MQ=255)
gTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGc < 1:4799721/71‑1 (MQ=255)
tttGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCt < 1:2847430/61‑1 (MQ=255)
ttgcttgcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTgg > 1:6298119/1‑62 (MQ=255)
tgcttgcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTcc > 1:6830366/1‑58 (MQ=255)
gcttgcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGt < 1:96418/70‑1 (MQ=255)
gcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCgg < 1:3297164/70‑1 (MQ=255)
gcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGc > 1:229637/1‑71 (MQ=255)
gACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGt > 1:12314/1‑49 (MQ=255)
gACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGGCCGGCTg > 1:2171792/1‑71 (MQ=255)
cATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc > 1:2083612/1‑70 (MQ=255)
tAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaa < 1:6306481/70‑1 (MQ=255)
tAAATTTCCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaa < 1:3888284/70‑1 (MQ=255)
aaaTTTGCGATGCAGGGTTACTACCCACCAGCACAAGGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaa < 1:5406922/69‑1 (MQ=255)
aaaTTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTg > 1:3106102/1‑48 (MQ=255)
aaaTTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTg > 1:2596756/1‑48 (MQ=255)
aaTTTGCGATGCAGGGGTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAc < 1:7538049/71‑1 (MQ=255)
aTTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAcg > 1:5015731/1‑71 (MQ=255)
aTTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAc < 1:3949223/70‑1 (MQ=255)
tttGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAcgc < 1:1083628/71‑1 (MQ=255)
tttGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAcgc < 1:7077826/71‑1 (MQ=255)
tttGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAcgc < 1:2592411/71‑1 (MQ=255)
ttGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCa > 1:6758665/1‑53 (MQ=255)
aTGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCt < 1:5650744/38‑1 (MQ=255)
aTGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAGTACGCGCCtta < 1:5443435/71‑3 (MQ=255)
aTGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGa < 1:7453829/71‑1 (MQ=255)
gCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGaa > 1:7686930/1‑70 (MQ=255)
gCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAAc > 1:1294413/1‑71 (MQ=255)
gggTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttt < 1:2790681/71‑1 (MQ=255)
gggTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttt < 1:1274880/71‑1 (MQ=255)
gTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCTCAGTCCGGCTGCAATACGCGCCTGAACttttt < 1:338742/71‑1 (MQ=255)
gTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttttt < 1:6290211/71‑1 (MQ=255)
actacCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAcgc > 1:4649594/1‑55 (MQ=255)
actacCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAcgc > 1:4213330/1‑55 (MQ=255)
ctacCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttt > 1:5402487/1‑65 (MQ=255)
caccaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACtt < 1:4068919/71‑1 (MQ=255)
accaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCa < 1:887582/66‑1 (MQ=255)
accaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACtt > 1:7517683/1‑70 (MQ=255)
ccaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACtttt < 1:5502530/59‑1 (MQ=255)
ccaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACtttt < 1:1817127/59‑1 (MQ=255)
ccaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCa < 1:4562670/71‑1 (MQ=255)
aGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCaga < 1:637782/71‑1 (MQ=255)
gCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACtt > 1:6203686/1‑66 (MQ=255)
gCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACt < 1:3199641/65‑1 (MQ=255)
cacaACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTg < 1:3017326/57‑1 (MQ=255)
acaACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAgc < 1:3087082/70‑1 (MQ=255)
caACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAgcgc < 1:6148268/71‑1 (MQ=255)
aaCGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACtt < 1:5390461/62‑1 (MQ=255)
aCGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttttt < 1:5434827/52‑1 (MQ=255)
cGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCa > 1:2998131/1‑56 (MQ=255)
cGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCAcc > 1:6250690/1‑71 (MQ=255)
ggCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttt > 1:5319033/1‑48 (MQ=255)
ggCCTGTCCT‑GTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACctg > 1:3879625/1‑71 (MQ=255)
|
CAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTG > W3110S.gb/556792‑556925
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
