Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F3 I0 R2
|
52 |
68.8 |
5189613 |
98.4% |
5106579 |
62.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
556,858 |
A→T |
100% |
L36Q (CTG→CAG) |
folD ← |
bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 556,858 | 0 | A | T | 100.0%
| 157.2
/ NA
| 53 | L36Q (CTG→CAG) | folD | bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (27/26); total (27/26) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTG > W3110S.gb/556790‑556925
|
cacaAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGt > 1:1297334/1‑71 (MQ=255)
acaAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGt < 1:3103707/70‑1 (MQ=255)
acaAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGt < 1:3935207/70‑1 (MQ=255)
acaAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTc > 1:4558989/1‑71 (MQ=255)
gCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTgg < 1:4830272/71‑1 (MQ=255)
cTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTg < 1:5035275/71‑1 (MQ=255)
ttGCGTTTGCTTGCGACATAATTTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTg < 1:2288017/70‑1 (MQ=255)
gCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGAACAACGGCCTGTCCTgg > 1:4964857/1‑66 (MQ=255)
gcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGc > 1:2683098/1‑71 (MQ=255)
gcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGc > 1:4571453/1‑71 (MQ=255)
cATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc > 1:1050696/1‑70 (MQ=255)
cATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc > 1:443941/1‑70 (MQ=255)
cATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc > 1:2059075/1‑70 (MQ=255)
aTAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTc < 1:1672118/47‑1 (MQ=255)
aTAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaa < 1:4154624/71‑1 (MQ=255)
aTAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaa > 1:299841/1‑71 (MQ=255)
tAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTc < 1:301010/46‑1 (MQ=255)
aaaTTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCa > 1:3189165/1‑57 (MQ=255)
aaTTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCAGGCTGCAATAc > 1:2812396/1‑71 (MQ=255)
aTTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAcg > 1:2854551/1‑71 (MQ=255)
tttGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAcgc < 1:3834754/71‑1 (MQ=255)
tttGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAcgc < 1:1191104/71‑1 (MQ=255)
cGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGcc < 1:1721949/70‑1 (MQ=255)
cGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCt > 1:853613/1‑71 (MQ=255)
cGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCt > 1:2368792/1‑71 (MQ=255)
gATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTg < 1:5092404/71‑1 (MQ=255)
tGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTg > 1:2597609/1‑38 (MQ=255)
gCAGGGTTACTACCCACCAGCACAACGGCCTGTcc < 1:4457258/35‑1 (MQ=255)
cAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCg > 1:3382324/1‑50 (MQ=255)
cAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCg > 1:3081454/1‑50 (MQ=255)
cAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACt > 1:471293/1‑71 (MQ=255)
ggTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACtttt < 1:2591275/71‑1 (MQ=255)
gTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttttt < 1:1060964/71‑1 (MQ=255)
gTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttttt < 1:1440045/71‑1 (MQ=255)
gTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttttt > 1:3884675/1‑71 (MQ=255)
gTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACtttt > 1:644875/1‑70 (MQ=255)
tactacCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaa > 1:4843548/1‑51 (MQ=255)
actacCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGa < 1:2094492/70‑1 (MQ=255)
tacCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTg < 1:1757712/58‑1 (MQ=255)
cccACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACtttt > 1:2765615/1‑63 (MQ=255)
cccACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCaa < 1:4101774/70‑1 (MQ=255)
cccACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAAc < 1:3349491/71‑1 (MQ=255)
accaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGc < 1:1351374/65‑1 (MQ=255)
accaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACtt > 1:3763551/1‑70 (MQ=255)
caGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACtt > 1:4716402/1‑68 (MQ=255)
cacaACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAgc < 1:678077/71‑1 (MQ=255)
cacaACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAgc < 1:2923024/71‑1 (MQ=255)
caACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAgcgc < 1:4502128/71‑1 (MQ=255)
aaCGGCCTGTCCTGGTGCCCGCAGTGCGGCTGCAATACGCGCCTGAACTTTTTGAGc < 1:4699307/57‑1 (MQ=255)
aaCGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGc < 1:2581922/57‑1 (MQ=255)
aCGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAcgc > 1:1575483/1‑39 (MQ=255)
cGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAg > 1:4855046/1‑54 (MQ=255)
gCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACctg > 1:4633170/1‑71 (MQ=255)
|
CACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTG > W3110S.gb/556790‑556925
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
