Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F8 I0 R2
|
53 |
71.6 |
5374071 |
98.5% |
5293459 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
556,858 |
A→T |
100% |
L36Q (CTG→CAG) |
folD ← |
bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 556,858 | 0 | A | T | 100.0%
| 205.0
/ NA
| 68 | L36Q (CTG→CAG) | folD | bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (32/36); total (32/36) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCT > W3110S.gb/556790‑556924
|
cacaAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGt > 1:958239/1‑71 (MQ=255)
cacaAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGt > 1:681774/1‑71 (MQ=255)
cacaAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGt > 1:4740563/1‑71 (MQ=255)
cacaAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGt > 1:3043291/1‑71 (MQ=255)
acaAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGt < 1:3669600/70‑1 (MQ=255)
caAGCCTTGCGTTTGCTTGCTACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTcc < 1:2714225/71‑1 (MQ=255)
caAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTcc < 1:50818/71‑1 (MQ=255)
aaGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCt < 1:42059/71‑1 (MQ=255)
aGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTg < 1:5068395/71‑1 (MQ=255)
aGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTg < 1:2699063/71‑1 (MQ=255)
aGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTg < 1:1939917/71‑1 (MQ=255)
tGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGcc > 1:3822953/1‑71 (MQ=255)
gCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGccc > 1:853071/1‑71 (MQ=255)
gCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGccc > 1:1196611/1‑71 (MQ=255)
cGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCg < 1:5013821/71‑1 (MQ=255)
cGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCg < 1:1531594/71‑1 (MQ=255)
gTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTg < 1:4637245/63‑1 (MQ=255)
tgcttgcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGt > 1:1121559/1‑71 (MQ=255)
tgcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCgg > 1:3831435/1‑71 (MQ=255)
gcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCt > 1:253336/1‑54 (MQ=255)
gcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGc > 1:657785/1‑71 (MQ=255)
gcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGc > 1:4993914/1‑71 (MQ=255)
aCATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc < 1:4934644/71‑1 (MQ=255)
cATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTc > 1:1313092/1‑48 (MQ=255)
tAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTcc < 1:1781501/62‑1 (MQ=255)
tAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTcc < 1:1787932/62‑1 (MQ=255)
tAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaa > 1:5318479/1‑70 (MQ=255)
aaaTTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTc > 1:2184137/1‑45 (MQ=255)
tttGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTg < 1:5352986/45‑1 (MQ=255)
tttGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTg < 1:4264431/45‑1 (MQ=255)
tttGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTg < 1:3937512/45‑1 (MQ=255)
gATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCt > 1:2677559/1‑70 (MQ=255)
gATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTg < 1:45456/71‑1 (MQ=255)
tGCAGGGTTACTACCCACCAGCACAACGGCCTGTc > 1:4177273/1‑35 (MQ=255)
tGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGaa > 1:1279687/1‑71 (MQ=255)
gCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGa > 1:1756784/1‑69 (MQ=255)
cAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACt < 1:1370866/71‑1 (MQ=255)
cAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACt > 1:4701032/1‑71 (MQ=255)
gggTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAc > 1:4622762/1‑58 (MQ=255)
gggTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttt < 1:683029/71‑1 (MQ=255)
gggTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttt < 1:1928172/71‑1 (MQ=255)
gggTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGAAGTCCGGCTGCAATACGCGCCTGAACttt < 1:2479941/71‑1 (MQ=255)
ggTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACtttt > 1:183374/1‑71 (MQ=255)
gTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttttt < 1:5025846/71‑1 (MQ=255)
ttACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTg < 1:1405737/71‑1 (MQ=255)
tactacCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttttt > 1:786072/1‑69 (MQ=255)
actacCCACCAGCACAACGGCTTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGa < 1:5057389/70‑1 (MQ=255)
actacCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGa < 1:1087054/70‑1 (MQ=255)
actacCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAg > 1:1500421/1‑71 (MQ=255)
actacCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAg > 1:3877394/1‑71 (MQ=255)
tacCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttttt < 1:811214/66‑1 (MQ=255)
acCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCaa > 1:4462198/1‑71 (MQ=255)
ccagcaGCACAACGGCCTGGCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttt < 1:1410535/61‑1 (MQ=255)
caccaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGaa > 1:1675478/1‑56 (MQ=255)
accaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTc < 1:1538602/71‑1 (MQ=255)
ccaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCa < 1:4271352/71‑1 (MQ=255)
ccaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCa < 1:3179991/71‑1 (MQ=255)
ccaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCa < 1:2121554/71‑1 (MQ=255)
caACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAgcgc < 1:942652/71‑1 (MQ=255)
aaCGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAAc < 1:2131540/60‑1 (MQ=255)
aaCGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCag < 1:3606813/65‑1 (MQ=255)
aaCGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCa > 1:2081082/1‑71 (MQ=255)
aaCGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCa > 1:5024390/1‑71 (MQ=255)
aaCGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCa > 1:1796818/1‑71 (MQ=255)
aaCGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCa > 1:4238634/1‑71 (MQ=255)
aCGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAAt > 1:2302820/1‑35 (MQ=255)
cGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCag < 1:3425908/63‑1 (MQ=255)
ggCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACct < 1:4363630/71‑1 (MQ=255)
|
CACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCT > W3110S.gb/556790‑556924
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
