Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F17 I0 R2
|
159 |
90.8 |
4763082 |
92.7% |
4415377 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,735,602 |
T→C |
11.0% |
E157E (GAA→GAG) |
era ← |
membrane‑associated, 16S rRNA‑binding GTPase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,735,602 | 0 | T | C | 11.0%
| 227.5
/ 17.7
| 82 | E157E (GAA→GAG) | era | membrane‑associated, 16S rRNA‑binding GTPase |
| Reads supporting (aligned to +/- strand): ref base T (34/39); new base C (5/4); total (39/43) |
| Fisher's exact test for biased strand distribution p-value = 7.30e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01 |
GGAAGTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCGGCAGCAG > minE/1735532‑1735670
|
ggAAGTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGtt > 1:2260312/1‑71 (MQ=255)
ggAAGTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGtt > 1:4040201/1‑71 (MQ=255)
gAAGTGATGAGTCGCTTCAGGTAGATGCTTACGCCCGATTGCCGCAATAGTGTCAACATTCAGCCCGGttt > 1:1436114/1‑71 (MQ=255)
gTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCgg > 1:542882/1‑71 (MQ=255)
atgaGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGc < 1:286712/69‑1 (MQ=255)
atgaGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGc < 1:4414510/69‑1 (MQ=255)
tgaGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGc < 1:1767592/68‑1 (MQ=255)
cGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTg > 1:2309235/1‑71 (MQ=255)
cGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTg > 1:1961905/1‑71 (MQ=255)
gCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTgg < 1:3054077/71‑1 (MQ=255)
gCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTgg < 1:1564464/71‑1 (MQ=255)
cTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGc < 1:1535428/71‑1 (MQ=255)
cTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGc < 1:3376615/71‑1 (MQ=255)
cAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCa > 1:2569818/1‑59 (MQ=255)
cAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCa > 1:3835602/1‑59 (MQ=255)
cAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCa > 1:4706895/1‑59 (MQ=255)
cAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCa > 1:4687029/1‑59 (MQ=255)
tAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGatat < 1:4307365/71‑1 (MQ=255)
gATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCATAGATTGGCACGATATc < 1:3383294/70‑1 (MQ=255)
gATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATc < 1:3763256/70‑1 (MQ=255)
aTGCTTACGGACGATTGCCGCAATAGTGTCAACATTCAGCCCGGttt < 1:3146755/47‑1 (MQ=255)
aTGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGttt < 1:3352437/47‑1 (MQ=255)
aTGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGttt < 1:3813019/47‑1 (MQ=255)
aTGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGttt < 1:3109318/47‑1 (MQ=255)
aTGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGttt < 1:2664069/47‑1 (MQ=255)
aTGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGttt < 1:3060940/47‑1 (MQ=255)
aTGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGttt < 1:2061434/47‑1 (MQ=255)
aTGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGttt < 1:785108/47‑1 (MQ=255)
gCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAg > 1:1217208/1‑70 (MQ=255)
gCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAg > 1:3627026/1‑70 (MQ=255)
gCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAg > 1:3826865/1‑70 (MQ=255)
gCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAg > 1:3451761/1‑70 (MQ=255)
gCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAg > 1:2659620/1‑70 (MQ=255)
ttACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGa < 1:1005742/67‑1 (MQ=255)
ttACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAgg < 1:3558688/69‑1 (MQ=255)
cGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGtt > 1:1408170/1‑71 (MQ=255)
cGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGtt > 1:2573264/1‑71 (MQ=255)
cGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGt > 1:3943847/1‑70 (MQ=255)
cGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGt > 1:3761683/1‑70 (MQ=255)
cGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAgaga > 1:4653903/1‑49 (MQ=255)
cGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAgaga > 1:4269339/1‑49 (MQ=255)
cGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAgaga > 1:3282150/1‑49 (MQ=255)
cACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTc > 1:2640955/1‑70 (MQ=255)
cACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCa < 1:3969772/71‑1 (MQ=255)
cACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCa < 1:2152006/71‑1 (MQ=255)
cACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCa < 1:588615/71‑1 (MQ=255)
cACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCa < 1:2468675/71‑1 (MQ=255)
aCGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCa < 1:374947/70‑1 (MQ=255)
cGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATc > 1:2228539/1‑71 (MQ=255)
gATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCt < 1:1248830/71‑1 (MQ=255)
gATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCt < 1:3814548/71‑1 (MQ=255)
gATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCt < 1:3327859/71‑1 (MQ=255)
gATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCt > 1:194349/1‑71 (MQ=255)
gATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCt < 1:2515718/71‑1 (MQ=255)
gATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCt < 1:2841964/71‑1 (MQ=255)
aTTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCAt > 1:3269167/1‑68 (MQ=255)
aTTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCAt > 1:3031455/1‑68 (MQ=255)
aTTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTg > 1:1443294/1‑71 (MQ=255)
ccGCAATAGTGTCAACATTCAGCCCGGTTTCGGCtgag < 1:3771919/38‑1 (MQ=255)
gCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGAt > 1:3716053/1‑38 (MQ=255)
gCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGc < 1:4698178/71‑1 (MQ=255)
gCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGc < 1:2284834/71‑1 (MQ=255)
gCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGc < 1:433706/71‑1 (MQ=255)
gCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAgg < 1:3322933/54‑1 (MQ=255)
tAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAgg < 1:803499/71‑1 (MQ=255)
tgtCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTg < 1:363288/63‑1 (MQ=255)
tgtCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTg < 1:4601650/63‑1 (MQ=255)
tgtCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTc < 1:4545974/53‑1 (MQ=255)
tgtCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTc < 1:2809755/53‑1 (MQ=255)
tCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGcc > 1:814491/1‑63 (MQ=255)
aaCATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTg > 1:751647/1‑69 (MQ=255)
aaCATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTg > 1:1519449/1‑69 (MQ=255)
aaCATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTg > 1:515470/1‑69 (MQ=255)
aaCATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTg > 1:2101028/1‑69 (MQ=255)
aaCATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGc < 1:4241739/70‑1 (MQ=255)
aTTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGaa > 1:1907088/1‑42 (MQ=255)
aTTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGaa > 1:3098239/1‑42 (MQ=255)
aTTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGaa > 1:2573847/1‑42 (MQ=255)
ttCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAgg < 1:1793153/39‑1 (MQ=255)
ttCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAgg < 1:2835376/39‑1 (MQ=255)
ttCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTg > 1:3408008/1‑71 (MQ=255)
cAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGaa > 1:4454215/1‑39 (MQ=255)
aGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGaa > 1:829221/1‑59 (MQ=255)
aGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGaa > 1:3710521/1‑59 (MQ=255)
aGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCgg < 1:1045645/71‑1 (MQ=255)
ccGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCGgcag < 1:4226239/71‑1 (MQ=255)
ccGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCGgcag < 1:3473044/71‑1 (MQ=255)
ccGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCGgcag < 1:1938032/71‑1 (MQ=255)
ccGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCGgcag < 1:112932/71‑1 (MQ=255)
cGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGc < 1:255252/59‑1 (MQ=255)
ggTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCt < 1:3961440/41‑1 (MQ=255)
ggTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCGgcag > 1:1604827/1‑69 (MQ=255)
gTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCGGcagcag < 1:2789403/71‑1 (MQ=255)
tttCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCGgcag > 1:499856/1‑67 (MQ=255)
|
GGAAGTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCGGCAGCAG > minE/1735532‑1735670
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
