Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I2 R2
|
64 |
60.0 |
3197630 |
92.7% |
2964203 |
60.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
1,735,602 |
T→C |
E157E (GAA→GAG) |
era ← |
membrane‑associated, 16S rRNA‑binding GTPase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,735,602 | 0 | T | C | 100.0%
| 227.5
/ NA
| 61 | E157E (GAA→GAG) | era | membrane‑associated, 16S rRNA‑binding GTPase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (36/25); total (36/25) |
AGTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCG > minE/1735535‑1735663
|
aGTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCg < 1:419118/71‑1 (MQ=255)
aGTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCg > 1:388639/1‑71 (MQ=255)
gTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCgg > 1:1600121/1‑71 (MQ=255)
gTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCgg < 1:1778950/71‑1 (MQ=255)
gatgaGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCgg > 1:2397564/1‑69 (MQ=255)
atgaGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCgg > 1:2405795/1‑68 (MQ=255)
aGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCagag < 1:1216473/70‑1 (MQ=255)
aGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCagag < 1:245883/70‑1 (MQ=255)
aGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCa > 1:761645/1‑67 (MQ=255)
gTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGAt < 1:210125/71‑1 (MQ=255)
gCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTgg > 1:2618941/1‑71 (MQ=255)
gCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTgg > 1:2041546/1‑71 (MQ=255)
ttCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCagag < 1:3077170/64‑1 (MQ=255)
cAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGAt > 1:2601633/1‑64 (MQ=255)
cAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCa < 1:2624819/69‑1 (MQ=255)
cAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCa < 1:2550575/69‑1 (MQ=255)
aGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACg < 1:2150054/70‑1 (MQ=255)
gTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGata > 1:685246/1‑71 (MQ=255)
gTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGata > 1:834400/1‑71 (MQ=255)
gTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGata > 1:2493306/1‑71 (MQ=255)
gTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGat > 1:428492/1‑70 (MQ=255)
aGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGa > 1:695101/1‑67 (MQ=255)
aGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGa > 1:2045815/1‑67 (MQ=255)
ttACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAgg > 1:2316884/1‑69 (MQ=255)
aCGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAg < 1:2159321/70‑1 (MQ=255)
gCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTc < 1:2834769/71‑1 (MQ=255)
cACGATTGCCGCAATAGTGTCAACATTCAGCTCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCa < 1:2695606/71‑1 (MQ=255)
cACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCa < 1:2058145/71‑1 (MQ=255)
cACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCa < 1:2340028/71‑1 (MQ=255)
aCGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCa < 1:1652850/70‑1 (MQ=255)
gATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCt > 1:2160482/1‑71 (MQ=255)
aTTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTg > 1:1040080/1‑71 (MQ=255)
ccGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACTATATCGAGGAAGTTCATCTgg < 1:1332419/68‑1 (MQ=255)
cAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGAt > 1:386437/1‑37 (MQ=255)
cAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGc > 1:2102061/1‑70 (MQ=255)
cAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGc > 1:723086/1‑70 (MQ=255)
cAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATATGGCTTGc > 1:2586668/1‑70 (MQ=255)
tCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACt < 1:815340/70‑1 (MQ=255)
tCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACt < 1:417022/70‑1 (MQ=255)
aaCATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGcc < 1:1323238/61‑1 (MQ=255)
aaCATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGcc < 1:2788959/61‑1 (MQ=255)
aaCATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGcc < 1:43284/61‑1 (MQ=255)
aCATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGcc < 1:1964292/60‑1 (MQ=255)
cATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCAt > 1:637570/1‑49 (MQ=255)
cATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCAt > 1:56477/1‑49 (MQ=255)
cATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAgg > 1:1629577/1‑71 (MQ=255)
cATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAgg > 1:2905866/1‑71 (MQ=255)
aTTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGt > 1:1768159/1‑71 (MQ=255)
aTTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGt < 1:2212577/71‑1 (MQ=255)
ttCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATc < 1:2291468/48‑1 (MQ=255)
ttCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAg > 1:2929809/1‑68 (MQ=255)
tCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTc < 1:2722879/44‑1 (MQ=255)
tCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGc > 1:1863666/1‑71 (MQ=255)
cAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCg > 1:1551773/1‑71 (MQ=255)
aGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCAt > 1:1103039/1‑44 (MQ=255)
aGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCAt > 1:579544/1‑44 (MQ=255)
aGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCAt > 1:3025873/1‑44 (MQ=255)
aGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCAt > 1:2667318/1‑44 (MQ=255)
aGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCAt > 1:174396/1‑44 (MQ=255)
aGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCAt > 1:1830756/1‑44 (MQ=255)
ccGGTCTCGGCAGACATTGGCACGATATCGAGGaa > 1:468586/1‑35 (MQ=255)
|
AGTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCG > minE/1735535‑1735663
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
