Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F17 I0 R2
|
159 |
90.8 |
4763082 |
92.7% |
4415377 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,973,690 |
A→T |
11.3% |
K29I (AAA→ATA) |
nadR → |
bifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,973,690 | 0 | A | T | 11.3%
| 176.0
/ 12.5
| 62 | K29I (AAA→ATA) | nadR | bifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (25/30); new base T (5/2); total (30/32) |
| Fisher's exact test for biased strand distribution p-value = 2.49e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTG > minE/2973625‑2973757
|
gAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCaaa > 1:841307/1‑67 (MQ=255)
gAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCaaa > 1:3095208/1‑67 (MQ=255)
gAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCaaa > 1:4000012/1‑67 (MQ=255)
tCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGc < 1:2582822/68‑1 (MQ=255)
tCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGc < 1:2279342/68‑1 (MQ=255)
tCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGc < 1:4048156/68‑1 (MQ=255)
tCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAg > 1:3404156/1‑71 (MQ=255)
tCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAg > 1:111352/1‑71 (MQ=255)
cAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGt > 1:4372720/1‑71 (MQ=255)
aaGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAg > 1:3354259/1‑69 (MQ=255)
aaGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAg > 1:1843355/1‑69 (MQ=255)
aaGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAg > 1:4593447/1‑69 (MQ=255)
aaGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAg > 1:2880836/1‑69 (MQ=255)
aaGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGt > 1:3457352/1‑70 (MQ=255)
aaGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGt < 1:983123/70‑1 (MQ=255)
aaGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGt > 1:3201927/1‑70 (MQ=255)
aaGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGt > 1:2648287/1‑70 (MQ=255)
aaGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGt > 1:4618485/1‑70 (MQ=255)
gCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTAc > 1:1350745/1‑71 (MQ=255)
gCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTAc > 1:3964012/1‑71 (MQ=255)
aaCAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTg < 1:2603542/71‑1 (MQ=255)
aaCAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTg < 1:2687234/71‑1 (MQ=255)
aaCAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACt > 1:3754240/1‑70 (MQ=255)
gggCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACt < 1:2150659/66‑1 (MQ=255)
tGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGt < 1:1205862/58‑1 (MQ=255)
tGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGt < 1:3289576/58‑1 (MQ=255)
tGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGt < 1:2566672/58‑1 (MQ=255)
gCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGt < 1:1273889/45‑1 (MQ=255)
cGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATc < 1:1817518/71‑1 (MQ=255)
cGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATc < 1:3437686/71‑1 (MQ=255)
gCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGGTACTGAATGCCAAAATCa < 1:270701/71‑1 (MQ=255)
tACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCaa > 1:4436495/1‑70 (MQ=255)
cagGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGccc < 1:4001756/71‑1 (MQ=255)
cagGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGccc < 1:2504196/71‑1 (MQ=255)
cagGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGccc < 1:799019/71‑1 (MQ=255)
cagGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGccc > 1:1963009/1‑71 (MQ=255)
tAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCa > 1:842106/1‑40 (MQ=255)
tAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCa > 1:3361736/1‑40 (MQ=255)
aGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAgcg > 1:1248488/1‑71 (MQ=255)
cTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTg < 1:4142309/44‑1 (MQ=255)
cTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAgcgcg > 1:4696916/1‑71 (MQ=255)
gATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGaa > 1:3341103/1‑44 (MQ=255)
gATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGaa > 1:4162558/1‑44 (MQ=255)
gATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAgcgcgc > 1:2249900/1‑70 (MQ=255)
tGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCaa > 1:16469/1‑70 (MQ=255)
gCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCaaa < 1:3937743/48‑1 (MQ=255)
gCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCaaa < 1:2543088/48‑1 (MQ=255)
ccAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAg < 1:3882356/71‑1 (MQ=255)
cAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGc < 1:3991995/71‑1 (MQ=255)
cAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGc < 1:2463657/71‑1 (MQ=255)
gCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTg > 1:971031/1‑71 (MQ=255)
gCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTg < 1:4001743/71‑1 (MQ=255)
gCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTg < 1:471845/71‑1 (MQ=255)
cGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGcc < 1:3229165/53‑1 (MQ=255)
cGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTgg < 1:1647885/71‑1 (MQ=255)
ggTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGa < 1:893179/71‑1 (MQ=255)
aTGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGc > 1:1662307/1‑71 (MQ=255)
tGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCaaaa < 1:1925584/39‑1 (MQ=255)
tGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCaaaa < 1:3800879/39‑1 (MQ=255)
tGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCg < 1:4255762/71‑1 (MQ=255)
tGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCg > 1:1464227/1‑71 (MQ=255)
tGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCg > 1:892185/1‑71 (MQ=255)
aCCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGt < 1:122715/70‑1 (MQ=255)
ccATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTg > 1:833165/1‑62 (MQ=255)
cAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGGGCGCAAAAGCTGGAGGCGTTg > 1:4198935/1‑70 (MQ=255)
|
GAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTG > minE/2973625‑2973757
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
