Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R2
|
370 |
72.1 |
3738297 |
91.5% |
3420541 |
62.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,973,690 |
A→T |
55.7% |
K29I (AAA→ATA) |
nadR → |
bifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,973,690 | 0 | A | T | 55.7%
| 30.6
/ 106.0
| 79 | K29I (AAA→ATA) | nadR | bifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (17/18); new base T (21/23); total (38/41) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 |
CCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTG > minE/2973622‑2973757
|
ccTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCaaa < 1:563701/70‑1 (MQ=255)
ccTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCaaa < 1:3694170/70‑1 (MQ=255)
gAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAggg > 1:1055027/1‑70 (MQ=255)
gAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAggg > 1:1060603/1‑70 (MQ=255)
aaaCTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTAt > 1:384931/1‑71 (MQ=255)
aCTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTAttt < 1:107230/71‑1 (MQ=255)
aCTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTAttt < 1:3345689/71‑1 (MQ=255)
ccATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTaa < 1:1354414/69‑1 (MQ=255)
ccATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTaa < 1:2518960/69‑1 (MQ=255)
cATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGcc < 1:2471283/71‑1 (MQ=255)
tCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAg > 1:2217592/1‑71 (MQ=255)
aGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTa > 1:2387074/1‑71 (MQ=255)
gCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTa > 1:173983/1‑70 (MQ=255)
gCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTAc > 1:2687423/1‑71 (MQ=255)
gCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTAc > 1:3287556/1‑71 (MQ=255)
cAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGaa < 1:2362100/71‑1 (MQ=255)
ggCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGt < 1:2807300/61‑1 (MQ=255)
cTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGaa > 1:1769551/1‑66 (MQ=255)
cTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGaa > 1:2492161/1‑66 (MQ=255)
cTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCa < 1:2763432/71‑1 (MQ=255)
cTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCa < 1:2050460/71‑1 (MQ=255)
gCACGCTACAGCAGGTAGCTTATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCa > 1:2830832/1‑69 (MQ=255)
gCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCa > 1:3103050/1‑69 (MQ=255)
cACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCaaa < 1:71493/70‑1 (MQ=255)
aggCTACAGCAGGTAGCTGATGCCAGCGGGATGACCAAAGGGTa < 1:2275915/42‑1 (MQ=38)
aCGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGaa > 1:2649642/1‑62 (MQ=255)
aCGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTa < 1:3194962/44‑1 (MQ=255)
aCGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTa < 1:2868149/44‑1 (MQ=255)
aCGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTa < 1:3693999/44‑1 (MQ=255)
aCGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTa < 1:1320838/44‑1 (MQ=255)
cGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATc < 1:1219814/71‑1 (MQ=255)
gCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCa < 1:401423/71‑1 (MQ=255)
gCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTg < 1:2447591/58‑1 (MQ=255)
tACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCaa > 1:240232/1‑70 (MQ=255)
tACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCaa > 1:2622909/1‑70 (MQ=255)
tACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCaa > 1:1521683/1‑70 (MQ=255)
tACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTaa > 1:1737205/1‑45 (MQ=255)
tACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAg > 1:884078/1‑50 (MQ=255)
tACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAg > 1:1193365/1‑50 (MQ=255)
tACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAg > 1:1740142/1‑50 (MQ=255)
aCAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCGAGt > 1:2863608/1‑50 (MQ=255)
aCAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGt > 1:1726543/1‑50 (MQ=255)
aCAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGt > 1:3464696/1‑50 (MQ=255)
aCAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGt > 1:1124875/1‑50 (MQ=255)
aCAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGt > 1:2765590/1‑50 (MQ=255)
aCAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCaa > 1:545714/1‑69 (MQ=255)
cagcagGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAg > 1:2244515/1‑71 (MQ=255)
cagcagGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGt > 1:1228954/1‑49 (MQ=255)
cagcagGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGt > 1:1253116/1‑49 (MQ=255)
cagcagGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGt > 1:2989565/1‑49 (MQ=255)
gcagGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGcc > 1:1858373/1‑71 (MQ=255)
agGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGcccc < 1:1027880/71‑1 (MQ=255)
gTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGt < 1:3723553/43‑1 (MQ=255)
gTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGt < 1:181582/43‑1 (MQ=255)
aGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAAt > 1:921068/1‑57 (MQ=255)
aGCTGACGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAgcg > 1:295072/1‑71 (MQ=255)
gCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCa < 1:98894/58‑1 (MQ=255)
gCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAgcg < 1:1733277/70‑1 (MQ=255)
tGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAAt < 1:820820/54‑1 (MQ=255)
tGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAgcgcg > 1:3396382/1‑70 (MQ=255)
gATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAAt < 1:587144/53‑1 (MQ=255)
aTGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCaa < 1:2117566/71‑1 (MQ=255)
aTGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCaa < 1:3070744/71‑1 (MQ=255)
ccAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCaaaa < 1:2112138/70‑1 (MQ=255)
ccAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCaaaa < 1:508107/70‑1 (MQ=255)
gCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTg < 1:1403841/71‑1 (MQ=255)
cGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCaaaa < 1:2959301/44‑1 (MQ=255)
cGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCaaaa < 1:1296024/44‑1 (MQ=255)
cGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCaaaa < 1:1386328/44‑1 (MQ=255)
cGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCaaaa < 1:2413490/44‑1 (MQ=255)
cGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCaaaa < 1:2511294/44‑1 (MQ=255)
cGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCaaaa < 1:2517054/44‑1 (MQ=255)
gTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATc > 1:3119305/1‑44 (MQ=255)
tATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAgg > 1:1912464/1‑71 (MQ=255)
tGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGc < 1:429552/70‑1 (MQ=255)
gACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGt > 1:2207571/1‑71 (MQ=255)
gACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGt > 1:2827725/1‑71 (MQ=255)
gACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATc < 1:3083011/40‑1 (MQ=255)
ccATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGtt < 1:1225801/70‑1 (MQ=255)
ccATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTg < 1:2979872/71‑1 (MQ=255)
ccATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTg < 1:3115420/71‑1 (MQ=255)
|
CCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTG > minE/2973622‑2973757
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
