Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I2 R2
|
64 |
60.0 |
3197630 |
92.7% |
2964203 |
60.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,973,690 |
A→T |
K29I (AAA→ATA) |
nadR → |
bifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,973,690 | 0 | A | T | 100.0%
| 179.6
/ NA
| 54 | K29I (AAA→ATA) | nadR | bifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (17/37); total (17/37) |
CTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTG > minE/2973623‑2973757
|
cTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAgg < 1:1207019/71‑1 (MQ=255)
cTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAgg < 1:1347212/71‑1 (MQ=255)
cTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAgg < 1:1491478/71‑1 (MQ=255)
aaaaCTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTa > 1:2919162/1‑71 (MQ=255)
aaaaCTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTa > 1:3119705/1‑71 (MQ=255)
aaaCTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTAt > 1:105248/1‑71 (MQ=255)
aCTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTAttt < 1:225268/71‑1 (MQ=255)
ccATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTaa < 1:1385006/69‑1 (MQ=255)
ccATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTaa < 1:194387/69‑1 (MQ=255)
ccATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGc < 1:324304/71‑1 (MQ=255)
aTCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGc > 1:616749/1‑69 (MQ=255)
gCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTAc < 1:1155189/71‑1 (MQ=255)
cAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGaa < 1:2002115/71‑1 (MQ=255)
cAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAAGGTATTTAAGCCAGTTACTGaa < 1:258975/71‑1 (MQ=255)
aGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTa < 1:1567058/56‑1 (MQ=255)
aGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTa < 1:2954155/56‑1 (MQ=255)
aGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTa < 1:2437657/56‑1 (MQ=255)
aGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTa < 1:1374112/56‑1 (MQ=255)
aGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTa < 1:1247936/56‑1 (MQ=255)
aGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTg < 1:796276/68‑1 (MQ=255)
ggCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGaa > 1:1499686/1‑68 (MQ=255)
gCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCaa > 1:680422/1‑70 (MQ=255)
gCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCaa > 1:2055535/1‑70 (MQ=255)
gCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCaa > 1:2524162/1‑70 (MQ=255)
cACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCaaaa > 1:2048855/1‑71 (MQ=255)
cACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCaaaa > 1:2806239/1‑71 (MQ=255)
cGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATc < 1:2375025/71‑1 (MQ=255)
gCTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCa < 1:796349/71‑1 (MQ=255)
cTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGa < 1:2490229/58‑1 (MQ=255)
cTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCaa < 1:584863/71‑1 (MQ=255)
cTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCaa < 1:2778824/71‑1 (MQ=255)
cTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCaa < 1:1358022/71‑1 (MQ=255)
cTACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCa > 1:989718/1‑70 (MQ=255)
tACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCaa > 1:2787984/1‑70 (MQ=255)
tACAGCAGGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCaa > 1:1660525/1‑70 (MQ=255)
cagcagGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCaaa < 1:2519156/69‑1 (MQ=255)
cagcagGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCaaa < 1:1507528/69‑1 (MQ=255)
gcagGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGcc < 1:2115686/71‑1 (MQ=255)
gcagGTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGcc < 1:260709/71‑1 (MQ=255)
gTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCaa < 1:1705998/56‑1 (MQ=255)
gTAGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCaa < 1:1874375/56‑1 (MQ=255)
aGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCaaaa > 1:1633471/1‑56 (MQ=255)
aGCTGATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCaaa > 1:707186/1‑55 (MQ=255)
gATGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAgcgcgc > 1:2780825/1‑70 (MQ=255)
aTGCCAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCaa < 1:972198/71‑1 (MQ=255)
ccAGCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAg < 1:101924/71‑1 (MQ=255)
gCGGTATGACCATATGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTg < 1:3070855/71‑1 (MQ=255)
gCGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTg < 1:229726/71‑1 (MQ=255)
cGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAATAGCTg < 1:1816837/70‑1 (MQ=255)
cGGTATGACCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTgg < 1:866293/71‑1 (MQ=255)
aCCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGt < 1:1000035/70‑1 (MQ=255)
aCCATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGt < 1:1519547/70‑1 (MQ=255)
ccATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTgg < 1:623815/63‑1 (MQ=255)
ccATAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTg > 1:427897/1‑71 (MQ=255)
|
CTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTG > minE/2973623‑2973757
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
