Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,303,633 |
G→T |
Q246Y (CAG→TAT) |
oppA → |
oligopeptide transporter subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,303,633 | 0 | G | T | 100.0%
| 87.2
/ NA
| 28 | Q246Y (CAG→TAT) | oppA | oligopeptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (0/28); total (0/28) |
GTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGA > W3110S.gb/1303580‑1303659
|
gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:1288050/62‑1 (MQ=255)
gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:1429757/62‑1 (MQ=255)
gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:1811027/62‑1 (MQ=255)
gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:181137/62‑1 (MQ=255)
gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:2006453/62‑1 (MQ=255)
gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:2934651/62‑1 (MQ=255)
gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:2811081/62‑1 (MQ=255)
gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:279505/62‑1 (MQ=255)
gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:2794183/62‑1 (MQ=255)
gTTCTTGAACGCAGCCCGACCTACTAGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:565087/62‑1 (MQ=255)
aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa < 1:1297932/62‑1 (MQ=255)
aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa < 1:688758/62‑1 (MQ=255)
aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa < 1:685097/62‑1 (MQ=255)
aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa < 1:422827/62‑1 (MQ=255)
aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa < 1:3120471/62‑1 (MQ=255)
aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa < 1:2861124/62‑1 (MQ=255)
aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa < 1:2707306/62‑1 (MQ=255)
aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa < 1:2445564/62‑1 (MQ=255)
aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa < 1:2342391/62‑1 (MQ=255)
aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa < 1:1823832/62‑1 (MQ=255)
aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa < 1:1641309/62‑1 (MQ=255)
ggAACAACGCGAAAACCGTTATTAACTATGTAACCt < 1:2490918/36‑1 (MQ=37)
ggAACAACGCGAAAACCGTTATTAACTATGTAACCt < 1:2878515/36‑1 (MQ=37)
ggAACAACGCGAAAACCGTTATTAACTATGTAACCt < 1:2419549/36‑1 (MQ=37)
ggAACAACGCGAAAACCGTTATTAACTATGTAACCt < 1:1914179/36‑1 (MQ=37)
ggAACAACGCGAAAACCGTTATTAACTATGTAACCt < 1:895878/36‑1 (MQ=37)
ggAACAACGCGAAAACCGTTATTAACTATGTAACCt < 1:989837/36‑1 (MQ=37)
tattaACTATGTAACCTATTTGCCTATTGCTTCTGa < 1:2376676/36‑1 (MQ=37)
|
GTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGA > W3110S.gb/1303580‑1303659
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
