Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I2 R2	64	60.0	3197630	92.7%	2964203	60.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	709,001	A→G	Y102H (TAT→CAT)	yceI ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	709,001	0	A	G	100.0%	254.0 / NA	68	Y102H (TAT→CAT)	yceI	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/46); total (22/46)

CGCCGGTAATATCCAGTTCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATATTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGTTCGGCGTGATTAGTATCGACG  >  minE/708935‑709068

cGCCGGTAATATCCAGTTCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGtttt                                                                 >  1:2076700/1‑71 (MQ=255)

 gCCGGTAATATCCAGTTCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTg                                                                <  1:1378781/71‑1 (MQ=255)

   cGGTAATATCCAGTTCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCg                                                              >  1:3151845/1‑71 (MQ=255)

      tAATATCCAGTTCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTa                                                           >  1:1128635/1‑71 (MQ=255)

         tatCCAGTTCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTAtt                                                         >  1:3093510/1‑70 (MQ=255)

          atCCAGTTCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTAtt                                                         >  1:491625/1‑69 (MQ=255)

             cAGTTCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAgg                                                     <  1:2702690/70‑1 (MQ=255)

             cAGTTCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAgg                                                     <  1:1117282/70‑1 (MQ=255)

              aGTTCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTTTTGAgg                                                     <  1:3278/69‑1 (MQ=255)

              aGTTCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAgg                                                     <  1:125330/69‑1 (MQ=255)

              aGTTCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGaa                                                   <  1:1890388/71‑1 (MQ=255)

                ttCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAAt                                                 >  1:2757291/1‑71 (MQ=255)

                 tCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAgg                                                     <  1:608713/66‑1 (MQ=255)

                 tCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAgg                                                     <  1:1980812/66‑1 (MQ=255)

                 tCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAgg                                                     <  1:2808237/66‑1 (MQ=255)

                  cGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATc                                                <  1:2904468/70‑1 (MQ=255)

                  cGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATc                                                <  1:787696/70‑1 (MQ=255)

                  cGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATc                                                <  1:2976140/70‑1 (MQ=255)

                  cGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATc                                                <  1:1698504/70‑1 (MQ=255)

                   gTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGaa                                                   >  1:2381442/1‑66 (MQ=255)

                   gTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGaa                                                   >  1:2475962/1‑66 (MQ=255)

                   gTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTg                                              <  1:1695872/71‑1 (MQ=255)

                       ccGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACt                                          <  1:1615261/71‑1 (MQ=255)

                       ccGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACt                                          <  1:1280934/71‑1 (MQ=255)

                        cGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGtt                                                                   <  1:2967728/45‑1 (MQ=255)

                         gTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGtt                                                                   <  1:1055135/44‑1 (MQ=255)

                          tctttcttCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGaa                                                   <  1:1193364/59‑1 (MQ=255)

                          tctttcttCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGaa                                                   <  1:692658/59‑1 (MQ=255)

                            tttcttCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCg                                       >  1:163549/1‑69 (MQ=255)

                              tcttcACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGa                                      <  1:823132/68‑1 (MQ=255)

                              tcttcACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAg                                    <  1:590815/70‑1 (MQ=255)

                               cttcACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGAt                                  >  1:181934/1‑71 (MQ=255)

                               cttcACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGAt                                  >  1:706418/1‑71 (MQ=255)

                                ttcACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGa                                                       <  1:1328724/49‑1 (MQ=255)

                                ttcACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGa                                                       <  1:475655/49‑1 (MQ=255)

                                ttcACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGa                                                       <  1:1467883/49‑1 (MQ=255)

                                 tcACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCa                                            >  1:2875631/1‑59 (MQ=255)

                                     gCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTAt                            <  1:571114/71‑1 (MQ=255)

                                       tggtggAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTAt                            <  1:1059094/69‑1 (MQ=255)

                                       tggtggAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATcg                          >  1:753146/1‑71 (MQ=255)

                                          tggAGGTGAATGTTGCCTGTGGATGTTTTGCGGTAtt                                                         <  1:478328/37‑1 (MQ=255)

                                          tggAGGTGAATGTTGCCTGTGGATGTTTTGCGGTAtt                                                         <  1:1385244/37‑1 (MQ=255)

                                          tggAGGTGAATGTTGCCTGTGGATGTTTTGCGGTAtt                                                         <  1:75285/37‑1 (MQ=255)

                                          tggAGGTGAATGTTGCCTGTGGATGTTTTGCGGTAtt                                                         <  1:1509326/37‑1 (MQ=255)

                                          tggAGGTGAATGTTGCCTGTGGATGTTTTGCGGTAtt                                                         <  1:586115/37‑1 (MQ=255)

                                          tggAGGTGAATGTTGCCTGTGGATGTTTTGCGGTAtt                                                         <  1:1618681/37‑1 (MQ=255)

                                          tggAGGTGAATGTTGCCTGTGGATGTTTTGCGGTAtt                                                         <  1:2101861/37‑1 (MQ=255)

                                          tggAGGTGAATGTTGCCTGTGGATGTTTTGCGGTAtt                                                         <  1:2483700/37‑1 (MQ=255)

                                          tggAGGTGAATGTTGCCTGTGGATGTTTTGCGGTAtt                                                         <  1:217027/37‑1 (MQ=255)

                                            gAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGTTc                     <  1:2358414/71‑1 (MQ=255)

                                              ggTGAATGTTGCCTGTGGATGTTTTGTGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGt                       >  1:732182/1‑67 (MQ=255)

                                              ggTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGTTCgg                   >  1:330041/1‑71 (MQ=255)

                                              ggTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGTTCgg                   >  1:2426326/1‑71 (MQ=255)

                                              ggTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGTTCgg                   >  1:654553/1‑71 (MQ=255)

                                               gTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCt                                               <  1:1916521/42‑1 (MQ=255)

                                               gTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCt                                               <  1:1471698/42‑1 (MQ=255)

                                               gTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCt                                               <  1:1692471/42‑1 (MQ=255)

                                               gTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCt                                               <  1:1903800/42‑1 (MQ=255)

                                                tGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCt                                               <  1:435099/41‑1 (MQ=255)

                                                 gAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCAc                                           <  1:1910285/44‑1 (MQ=255)

                                                 gAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCAc                                           <  1:2520282/44‑1 (MQ=255)

                                                  aaTGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGTTCGGCGt                <  1:851039/70‑1 (MQ=255)

                                                   aTGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGTTCGGCGTGa              >  1:1481310/1‑71 (MQ=255)

                                                      ttGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGt                       >  1:1088968/1‑59 (MQ=255)

                                                      ttGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGt                       >  1:2499249/1‑59 (MQ=255)

                                                      gtGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGt                       >  1:2927676/2‑59 (MQ=255)

                                                         ccTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGTTCGGCGTGATTAGt         <  1:1479781/70‑1 (MQ=255)

                                                               gATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGTTCGGCGTGATTAGTATCGACg  >  1:864899/1‑71 (MQ=255)

CGCCGGTAATATCCAGTTCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATATTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGTTCGGCGTGATTAGTATCGACG  >  minE/708935‑709068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A