Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R2	146	74.7	3299319	84.8%	2797822	66.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,099,149	A→G	T130A (ACA→GCA)	ydiM →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,099,149	0	A	G	100.0%	244.5 / NA	65	T130A (ACA→GCA)	ydiM	predicted transporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (0/65); total (0/65)

AACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATT  >  minE/1099086‑1099192

aaCAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCaa                                      <  1:766450/71‑1 (MQ=255)

aaCAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCaa                                      <  1:2409637/71‑1 (MQ=255)

aaCAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCaa                                      <  1:3148644/71‑1 (MQ=255)

   aGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAatat                                   <  1:1181181/71‑1 (MQ=255)

   aGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAatat                                   <  1:97892/71‑1 (MQ=255)

   aGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAatat                                   <  1:3141423/71‑1 (MQ=255)

   aGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAatat                                   <  1:1594321/71‑1 (MQ=255)

   aGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAatat                                   <  1:2659794/71‑1 (MQ=255)

   aGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAatat                                   <  1:2615170/71‑1 (MQ=255)

   aGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAatat                                   <  1:2611138/71‑1 (MQ=255)

            gATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTttaattaa                          <  1:2363135/71‑1 (MQ=255)

            gATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTttaattaa                          <  1:1212299/71‑1 (MQ=255)

            gATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTttaattaa                          <  1:246220/71‑1 (MQ=255)

            gATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTttaattaa                          <  1:2489099/71‑1 (MQ=255)

            gATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTttaattaa                          <  1:2727883/71‑1 (MQ=255)

            gATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTttaattaa                          <  1:2810337/71‑1 (MQ=255)

            gATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTttaattaa                          <  1:3269292/71‑1 (MQ=255)

            gATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTttaattaa                          <  1:684729/71‑1 (MQ=255)

                   gCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTaaa                         <  1:839470/65‑1 (MQ=255)

                   gCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTaaa                         <  1:3244136/65‑1 (MQ=255)

                   gCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTaaa                         <  1:2321905/65‑1 (MQ=255)

                   gCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTaaa                         <  1:1420700/65‑1 (MQ=255)

                   gCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTaaa                         <  1:344402/65‑1 (MQ=255)

                   gCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTaaa                         <  1:381740/65‑1 (MQ=255)

                   gCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTaaa                         <  1:495306/65‑1 (MQ=255)

                   gCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTaaa                         <  1:1857107/65‑1 (MQ=255)

                   gCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTaaa                         <  1:1926844/65‑1 (MQ=255)

                   gCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTaaa                         <  1:2211653/65‑1 (MQ=255)

                   gCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTaaa                         <  1:781575/65‑1 (MQ=255)

                   gCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTaaa                         <  1:97579/65‑1 (MQ=255)

                   gCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTaaa                         <  1:95704/65‑1 (MQ=255)

                                tttGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGAc      <  1:322596/71‑1 (MQ=255)

                                tttGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGAc      <  1:1318403/71‑1 (MQ=255)

                                tttGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGAc      <  1:780804/71‑1 (MQ=255)

                                tttGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGAc      <  1:2836138/71‑1 (MQ=255)

                                tttGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGAc      <  1:2868468/71‑1 (MQ=255)

                                tttGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGAc      <  1:1264931/71‑1 (MQ=255)

                                tttGATGGAAGCTTTTACACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGAc      <  1:2866435/71‑1 (MQ=255)

                                  tGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGAc      <  1:642473/69‑1 (MQ=255)

                                    aTGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtt  <  1:884977/71‑1 (MQ=255)

                                    aTGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtt  <  1:425765/71‑1 (MQ=255)

                                    aTGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtt  <  1:832511/71‑1 (MQ=255)

                                    aTGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtt  <  1:528421/71‑1 (MQ=255)

                                    aTGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtt  <  1:610295/71‑1 (MQ=255)

                                    aTGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtt  <  1:150498/71‑1 (MQ=255)

                                    aTGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtt  <  1:1516307/71‑1 (MQ=255)

                                    aTGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtt  <  1:2924184/71‑1 (MQ=255)

                                    aTGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtt  <  1:2751575/71‑1 (MQ=255)

                                    aTGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtt  <  1:2734679/71‑1 (MQ=255)

                                    aTGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtt  <  1:1733442/71‑1 (MQ=255)

                                    aTGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtt  <  1:2564123/71‑1 (MQ=255)

                                    aTGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtt  <  1:2552308/71‑1 (MQ=255)

                                    aTGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtt  <  1:248982/71‑1 (MQ=255)

                                    aTGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtt  <  1:2447232/71‑1 (MQ=255)

                                    aTGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAt   <  1:3118452/70‑1 (MQ=255)

                                    aTGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAt   <  1:1726785/70‑1 (MQ=255)

                                    aTGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAt   <  1:2798679/70‑1 (MQ=255)

                                    aTGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAt   <  1:2154999/70‑1 (MQ=255)

                                    aTGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGCACAAtt  <  1:420110/71‑1 (MQ=255)

                                     tGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtt  <  1:3218387/70‑1 (MQ=255)

                                     tGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtt  <  1:2182079/70‑1 (MQ=255)

                                        aaGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTaaa                         <  1:2099926/44‑1 (MQ=255)

                                                  aCGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGAc      <  1:1222020/53‑1 (MQ=255)

                                                        aCCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtt  <  1:37398/51‑1 (MQ=255)

                                                          cTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAt   <  1:508237/48‑1 (MQ=255)

AACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATT  >  minE/1099086‑1099192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A