Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R2
|
422 |
46.8 |
3726540 |
94.7% |
3529033 |
61.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,840,761 |
A→G |
89.8% |
E372E (GAA→GAG) |
ascB → |
cryptic 6‑phospho‑beta‑glucosidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,840,761 | 0 | A | G | 89.8%
| 176.0
/ 8.8
| 59 | E372E (GAA→GAG) | ascB | cryptic 6‑phospho‑beta‑glucosidase |
| Reads supporting (aligned to +/- strand): ref base A (2/4); new base G (26/27); total (28/31) |
| Fisher's exact test for biased strand distribution p-value = 6.73e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01 |
CGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCA > W3110S.gb/2840698‑2840822
|
cGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGcc > 1:3175167/1‑71 (MQ=255)
cGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGcc > 1:1306599/1‑71 (MQ=255)
cGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGc > 1:3085762/1‑70 (MQ=255)
tATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGTCCTGGGCGCaa < 1:989601/71‑1 (MQ=255)
tATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCaa < 1:3058619/71‑1 (MQ=255)
tATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCaa < 1:664937/71‑1 (MQ=255)
aCCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGagaa > 1:905480/1‑53 (MQ=255)
aCCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCaa > 1:476304/1‑67 (MQ=255)
aCCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCaa > 1:1199818/1‑67 (MQ=255)
aCCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCaa > 1:551438/1‑67 (MQ=255)
aTGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGACGCAAAAGATg > 1:3015769/1‑70 (MQ=255)
tGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGaa < 1:250722/71‑1 (MQ=255)
tGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGTGCGCAAAAGATGaa < 1:647456/71‑1 (MQ=255)
tGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGaa < 1:3694141/71‑1 (MQ=255)
tGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGaa < 1:3537263/71‑1 (MQ=255)
tGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGaa < 1:925862/71‑1 (MQ=255)
aaTATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAtt > 1:2412432/1‑71 (MQ=255)
aaTATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAtt > 1:330174/1‑71 (MQ=255)
tgatgTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAt > 1:263449/1‑66 (MQ=255)
tgTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:2229636/71‑1 (MQ=255)
tgTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:3113706/71‑1 (MQ=255)
tgTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:3312889/71‑1 (MQ=255)
tgTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:143052/71‑1 (MQ=255)
gTACGTTCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:1424907/70‑1 (MQ=255)
gTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc > 1:2748207/1‑70 (MQ=255)
gTACGATAGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc > 1:1241810/1‑70 (MQ=255)
tACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCaa < 1:3060360/71‑1 (MQ=255)
tACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCaa < 1:2890892/71‑1 (MQ=255)
tACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCaa < 1:2081117/71‑1 (MQ=255)
cGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGc > 1:655412/1‑69 (MQ=255)
cGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGc > 1:2568758/1‑69 (MQ=255)
gTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCaa > 1:2875847/1‑64 (MQ=255)
gTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga < 1:2292421/70‑1 (MQ=255)
gTTATAAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCaa > 1:2715679/1‑64 (MQ=255)
aTCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGAt > 1:3046835/1‑70 (MQ=255)
aGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAac > 1:170270/1‑71 (MQ=255)
aaGCCGCTGTTTCTGGTGGAGAACGGCCTTGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAacga > 1:3588274/1‑71 (MQ=255)
aaGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga < 1:2071026/62‑1 (MQ=255)
aaGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAacga > 1:1100832/1‑71 (MQ=255)
tttCTGGTGGAGAACGGCCTGGGCGCAAAAGATGa < 1:941928/35‑1 (MQ=255)
tttCTGGTGGAGAACGGCCTGGGCGCAAAAGATGa < 1:2441073/35‑1 (MQ=255)
tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAt > 1:21929/1‑35 (MQ=255)
tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCa > 1:2801436/1‑71 (MQ=255)
tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCa > 1:1098744/1‑71 (MQ=255)
tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCa > 1:2938143/1‑71 (MQ=255)
tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCa > 1:1255124/1‑71 (MQ=255)
tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCa > 1:1268576/1‑71 (MQ=255)
tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCa > 1:2785949/1‑71 (MQ=255)
tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCa > 1:1706385/1‑71 (MQ=255)
cTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCa < 1:2603691/70‑1 (MQ=255)
cTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCa < 1:647429/70‑1 (MQ=255)
cTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCa < 1:3200956/70‑1 (MQ=255)
cTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCa < 1:2779614/70‑1 (MQ=255)
cTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCa < 1:2903610/70‑1 (MQ=255)
tggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:509851/38‑1 (MQ=255)
tggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:2394010/38‑1 (MQ=255)
tggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:1777060/38‑1 (MQ=255)
tggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:1439847/38‑1 (MQ=255)
tggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:1002378/38‑1 (MQ=255)
tggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:3198972/38‑1 (MQ=255)
|
CGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCA > W3110S.gb/2840698‑2840822
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
