Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R2
|
356 |
55.1 |
4139924 |
95.4% |
3949487 |
63.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,840,761 |
A→G |
E372E (GAA→GAG) |
ascB → |
cryptic 6‑phospho‑beta‑glucosidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,840,761 | 0 | A | G | 100.0%
| 146.0
/ NA
| 41 | E372E (GAA→GAG) | ascB | cryptic 6‑phospho‑beta‑glucosidase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (7/34); total (7/34) |
GGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCATCAGC > W3110S.gb/2840699‑2840827
|
ggTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGc < 1:2005598/69‑1 (MQ=255)
tgTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:3260219/71‑1 (MQ=255)
tACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGagaa > 1:1065888/1‑38 (MQ=255)
tACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGagaa > 1:2850434/1‑38 (MQ=255)
tACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGagaa > 1:3097283/1‑38 (MQ=255)
tACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGagaa > 1:1407212/1‑38 (MQ=255)
tACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGagaa > 1:4017348/1‑38 (MQ=255)
cGATCGTTATCAGAAGCCGCTGTTTTTGGGGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATg < 1:2144341/71‑1 (MQ=255)
cGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATg < 1:13813/71‑1 (MQ=255)
cGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATg < 1:3082701/71‑1 (MQ=255)
cGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGATGCCAATg < 1:1855125/71‑1 (MQ=255)
gTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGa < 1:2073074/52‑1 (MQ=255)
gTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga < 1:3537251/70‑1 (MQ=255)
gTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga < 1:1600676/70‑1 (MQ=255)
gTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga < 1:2540800/70‑1 (MQ=255)
gTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga < 1:226969/70‑1 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGTACGGCCTGGGCGCAAAAGATGAAt > 1:1417980/1‑52 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAt > 1:4001681/1‑52 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAt > 1:3595421/1‑52 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAt > 1:2857927/1‑52 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAt > 1:800316/1‑52 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAt > 1:2106840/1‑52 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAt > 1:1654613/1‑52 (MQ=255)
tCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATTGCgag < 1:3406918/67‑1 (MQ=255)
tCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag < 1:2211708/67‑1 (MQ=255)
tCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag < 1:615767/67‑1 (MQ=255)
tCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag < 1:2517215/67‑1 (MQ=255)
tCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag < 1:2138322/67‑1 (MQ=255)
tCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag < 1:1973337/67‑1 (MQ=255)
tCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag < 1:3553899/67‑1 (MQ=255)
aaGCCGCTGTTTCTGGTGGAGAACGGCCTTGGCGCAAAAGATGAATTTGCTGcc < 1:3452970/54‑1 (MQ=255)
aaGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:1011424/54‑1 (MQ=255)
aaGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:1473390/54‑1 (MQ=255)
aaGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:3554924/54‑1 (MQ=255)
aaGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:3250681/54‑1 (MQ=255)
aaGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:2787437/54‑1 (MQ=255)
aaGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:2151167/54‑1 (MQ=255)
gCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAacgtcg < 1:1469173/71‑1 (MQ=255)
gCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAacgacg < 1:1703538/71‑1 (MQ=255)
gCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAacgacg < 1:1473955/71‑1 (MQ=255)
gCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAacgacg < 1:1077832/71‑1 (MQ=255)
gCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATgta < 1:2328201/40‑3 (MQ=39)
tggtggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag < 1:496681/50‑1 (MQ=255)
tggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCATCTGc < 1:719298/71‑1 (MQ=255)
tggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCATCAGc < 1:1719696/71‑1 (MQ=255)
tggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCATCAGc < 1:2921071/71‑1 (MQ=255)
|
GGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCATCAGC > W3110S.gb/2840699‑2840827
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
