Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F3 I0 R2
|
260 |
44.3 |
3360226 |
90.5% |
3041004 |
64.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,840,761 |
A→G |
44.2% |
E372E (GAA→GAG) |
ascB → |
cryptic 6‑phospho‑beta‑glucosidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,840,761 | 0 | A | G | 44.2%
| 23.8
/ 100.2
| 77 | E372E (GAA→GAG) | ascB | cryptic 6‑phospho‑beta‑glucosidase |
| Reads supporting (aligned to +/- strand): ref base A (18/25); new base G (11/23); total (29/48) |
| Fisher's exact test for biased strand distribution p-value = 4.80e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATC > W3110S.gb/2840695‑2840819
|
aCTCGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTTGAGAACg < 1:2676393/71‑1 (MQ=255)
aCTCGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACg < 1:1222806/71‑1 (MQ=255)
cTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACgg > 1:3158111/1‑65 (MQ=255)
cTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCt > 1:2853093/1‑68 (MQ=255)
cTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCt > 1:2896561/1‑68 (MQ=255)
cGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGgcg > 1:2893815/1‑70 (MQ=255)
cGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGgcg > 1:1741691/1‑70 (MQ=255)
tCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACg < 1:3145434/57‑1 (MQ=255)
tCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACg < 1:2727356/57‑1 (MQ=255)
tCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACg < 1:2774739/57‑1 (MQ=255)
tCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACg < 1:2585930/57‑1 (MQ=255)
tCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACg < 1:2430208/57‑1 (MQ=255)
tCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACg < 1:2172391/57‑1 (MQ=255)
tCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACg < 1:1950789/57‑1 (MQ=255)
tCACCATGAATATGATGTACGATCGATATCAGAAGCCGCTGTTTCTGGTGGAAAACg < 1:2483636/57‑1 (MQ=255)
tGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGaa < 1:220100/71‑1 (MQ=255)
tGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGaa < 1:2996176/71‑1 (MQ=255)
tGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGaa > 1:3053631/1‑71 (MQ=255)
tGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGaa > 1:3220246/1‑71 (MQ=255)
tGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGaa > 1:2578155/1‑71 (MQ=255)
tGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGaa > 1:144295/1‑71 (MQ=255)
tgatgTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACg > 1:1810750/1‑45 (MQ=255)
tgatgTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGcc > 1:481591/1‑48 (MQ=255)
tgatgTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCt > 1:1074351/1‑49 (MQ=255)
tgatgTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCt > 1:1886917/1‑49 (MQ=255)
tgatgTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCt > 1:3010679/1‑49 (MQ=255)
tgatgTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCt > 1:2676758/1‑49 (MQ=255)
tgatgTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCt > 1:234060/1‑49 (MQ=255)
tgatgTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCt > 1:468627/1‑49 (MQ=255)
gTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga < 1:35280/70‑1 (MQ=255)
gTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga < 1:959996/70‑1 (MQ=255)
gTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga < 1:3193423/70‑1 (MQ=255)
gTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga < 1:301823/70‑1 (MQ=255)
gTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga < 1:2321756/70‑1 (MQ=255)
gTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga < 1:2018637/70‑1 (MQ=255)
gTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga < 1:1618538/70‑1 (MQ=255)
gTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga < 1:1847604/70‑1 (MQ=255)
gTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga < 1:2956128/70‑1 (MQ=255)
ttATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga < 1:1264192/69‑1 (MQ=255)
ttATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTtgctgc < 1:139696/60‑1 (MQ=255)
ttATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTtgctgc < 1:1844885/60‑1 (MQ=255)
tCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGAtt < 1:2881279/70‑1 (MQ=255)
tCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGAtt < 1:2803241/70‑1 (MQ=255)
tCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGAtt < 1:3146562/70‑1 (MQ=255)
tCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGAtt < 1:3130347/70‑1 (MQ=255)
tCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGAtt < 1:2677295/70‑1 (MQ=255)
tCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGAtt < 1:2620702/70‑1 (MQ=255)
tCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGAtt < 1:396635/70‑1 (MQ=255)
tCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGAtt < 1:2189052/70‑1 (MQ=255)
tCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGAtt < 1:399689/70‑1 (MQ=255)
tCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGAtt < 1:1800896/70‑1 (MQ=255)
tCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGAtt < 1:1467621/70‑1 (MQ=255)
tCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGAtt < 1:764548/70‑1 (MQ=255)
tCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGAtt < 1:1232401/70‑1 (MQ=255)
tCAGAAGCCGCTGTTTCTGGAGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGAtt < 1:2366415/70‑1 (MQ=255)
gTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATc > 1:2706210/1‑71 (MQ=255)
tttCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAt < 1:590138/37‑1 (MQ=255)
tttCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAt < 1:60705/37‑1 (MQ=255)
tttCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAt < 1:997151/37‑1 (MQ=255)
tttCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAt < 1:1300731/37‑1 (MQ=255)
tttCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAt < 1:2409135/37‑1 (MQ=255)
tttCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAt < 1:1400343/37‑1 (MQ=255)
tttCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAt < 1:1742506/37‑1 (MQ=255)
ttCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAt < 1:3063822/36‑1 (MQ=255)
tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTtg > 1:1237922/1‑38 (MQ=255)
tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTtg > 1:897003/1‑38 (MQ=255)
tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTtg > 1:1365427/1‑38 (MQ=255)
tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTtg > 1:1460168/1‑38 (MQ=255)
tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTtg > 1:587271/1‑38 (MQ=255)
tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTtg > 1:169835/1‑38 (MQ=255)
tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTtg > 1:1930276/1‑38 (MQ=255)
tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTtg > 1:2219805/1‑38 (MQ=255)
tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTtg > 1:389304/1‑38 (MQ=255)
tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTtg > 1:2331861/1‑38 (MQ=255)
tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTtg > 1:2770137/1‑38 (MQ=255)
gtggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga < 1:2717929/47‑1 (MQ=255)
ggAAAACGGCCTGGGCGCAAAAGATTAATTTGCTGCCAATGGCGAGAtt < 1:2792243/49‑1 (MQ=255)
|
ACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATC > W3110S.gb/2840695‑2840819
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
