Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F17 I0 R2
|
276 |
45.3 |
3729697 |
93.0% |
3468618 |
60.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,840,761 |
A→G |
74.1% |
E372E (GAA→GAG) |
ascB → |
cryptic 6‑phospho‑beta‑glucosidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,840,761 | 0 | A | G | 74.1%
| 98.3
/ 32.6
| 54 | E372E (GAA→GAG) | ascB | cryptic 6‑phospho‑beta‑glucosidase |
| Reads supporting (aligned to +/- strand): ref base A (9/5); new base G (18/22); total (27/27) |
| Fisher's exact test for biased strand distribution p-value = 3.52e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
CGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCATCA > W3110S.gb/2840698‑2840825
|
cGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACgg > 1:1084067/1‑69 (MQ=255)
cGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACgg > 1:701528/1‑69 (MQ=255)
cGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACgg > 1:267648/1‑69 (MQ=255)
cGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACgg > 1:2304471/1‑69 (MQ=255)
cGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACg > 1:2498185/1‑68 (MQ=255)
tCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGcc < 1:2576093/68‑1 (MQ=255)
tCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGc < 1:970255/67‑1 (MQ=255)
tATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTggg < 1:3142693/66‑1 (MQ=255)
aCCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGgcgc > 1:643306/1‑65 (MQ=255)
aCCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGgcgc > 1:2939102/1‑65 (MQ=255)
aCCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGgcgc > 1:315845/1‑65 (MQ=255)
aCCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGgcgc > 1:1344186/1‑65 (MQ=255)
aCCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGgcg > 1:3698601/1‑64 (MQ=255)
aaTATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGa > 1:2428738/1‑68 (MQ=255)
atgatgTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAttt < 1:1971213/69‑1 (MQ=255)
atgatgTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAttt < 1:2769745/69‑1 (MQ=255)
atgatgTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAttt < 1:3153077/69‑1 (MQ=255)
atgatgTACGAGCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAttt < 1:1987002/69‑1 (MQ=38)
tACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:3426267/69‑1 (MQ=255)
tACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc > 1:3097541/1‑69 (MQ=255)
tACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc > 1:2857910/1‑69 (MQ=255)
tACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:2801216/69‑1 (MQ=255)
cGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGc < 1:80668/69‑1 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga < 1:3322219/68‑1 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga < 1:3624331/68‑1 (MQ=255)
tATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga < 1:1057611/68‑1 (MQ=255)
aTCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc > 1:3384601/1‑59 (MQ=255)
aTCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag > 1:2467669/1‑68 (MQ=255)
aTCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCgag > 1:527713/1‑68 (MQ=255)
aGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGAtt < 1:2634018/68‑1 (MQ=255)
aGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGAtt < 1:2073516/68‑1 (MQ=255)
aGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:2139206/56‑1 (MQ=255)
aGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:352871/56‑1 (MQ=255)
gCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAacg > 1:3449232/1‑68 (MQ=255)
ccGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAacgac < 1:906316/69‑1 (MQ=255)
ccGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAacgac < 1:2130830/69‑1 (MQ=255)
ccGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAacgac < 1:2353751/69‑1 (MQ=255)
tGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACt < 1:492809/69‑1 (MQ=255)
tGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACt < 1:3015397/69‑1 (MQ=255)
tGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACt < 1:1407015/69‑1 (MQ=255)
tGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACt < 1:3459354/69‑1 (MQ=255)
gTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATg > 1:1761517/1‑35 (MQ=255)
gTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATg > 1:1477748/1‑35 (MQ=255)
gTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATg > 1:3134361/1‑35 (MQ=255)
tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAac > 1:1098086/1‑58 (MQ=255)
tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACt > 1:2933501/1‑65 (MQ=255)
tCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCg > 1:3649382/1‑69 (MQ=255)
tCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCg > 1:2444769/1‑69 (MQ=255)
tggtggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGc < 1:1150748/68‑1 (MQ=255)
tggtggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTACCAATGGCGAGATTAACGACTACTATCGc < 1:3059795/68‑1 (MQ=255)
ggtggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCa < 1:1177663/68‑1 (MQ=255)
ggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCATCa > 1:3141268/1‑68 (MQ=255)
gAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc > 1:603077/1‑36 (MQ=255)
gAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc > 1:930228/1‑36 (MQ=255)
|
CGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCATCA > W3110S.gb/2840698‑2840825
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
