Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R2
|
356 |
55.1 |
4139924 |
95.4% |
3949487 |
63.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
515,934 |
C→T |
A43V (GCG→GTG) |
ybbM → |
predicted inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 515,934 | 0 | C | T | 100.0%
| 157.4
/ NA
| 52 | A43V (GCG→GTG) | ybbM | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (11/41); total (11/41) |
TTAATTAGCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGATGATG > W3110S.gb/515873‑515993
|
ttAATTAGCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAAtt > 1:3950006/1‑69 (MQ=255)
gCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGat > 1:2786847/1‑70 (MQ=255)
aaGAAAAACTGGCGCTGGATAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTc > 1:1774181/1‑71 (MQ=255)
aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTc > 1:2731123/1‑71 (MQ=255)
aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTc > 1:4022952/1‑71 (MQ=255)
aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTc > 1:3893150/1‑71 (MQ=255)
aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTc > 1:2697507/1‑71 (MQ=255)
aaGAAAAACTGGAGCTGGAGAAAGATATTCTCTGTAGCGTCGGGCGAGTGATaa > 1:834678/1‑54 (MQ=38)
aGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCg < 1:1889187/71‑1 (MQ=255)
aaaaCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCgg > 1:824633/1‑69 (MQ=255)
aaaaCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGc > 1:3146924/1‑70 (MQ=255)
cTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTAtgt > 1:2355177/1‑71 (MQ=255)
gcgcTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGc < 1:801639/70‑1 (MQ=255)
gcgcTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGc < 1:2243346/70‑1 (MQ=255)
gcgcTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGc < 1:1138507/70‑1 (MQ=255)
gcgcTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGc < 1:1625892/70‑1 (MQ=255)
gcgcTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGc < 1:271669/70‑1 (MQ=255)
cgcTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGc < 1:4052089/69‑1 (MQ=255)
gaAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGtata < 1:2119024/71‑1 (MQ=255)
gaAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGtata < 1:4117372/71‑1 (MQ=255)
aaaGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatt < 1:4003382/45‑1 (MQ=255)
aaaGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatt < 1:3705325/45‑1 (MQ=255)
aaaGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatt < 1:3419525/45‑1 (MQ=255)
aaaGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatt < 1:3199102/45‑1 (MQ=255)
aaaGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatt < 1:3180525/45‑1 (MQ=255)
aaaGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatt < 1:1659623/45‑1 (MQ=255)
aaaGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatt < 1:1682133/45‑1 (MQ=255)
aaaGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatt < 1:2289558/45‑1 (MQ=255)
aaaGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatt < 1:1001035/45‑1 (MQ=255)
aaaGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatt < 1:2654878/45‑1 (MQ=255)
aaaGATATTCTCTGGAGCGTCCGGCGAGTGATAATTCAGCTGatt < 1:2569341/45‑1 (MQ=255)
gATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCg < 1:616316/49‑1 (MQ=255)
ttCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGta < 1:3303602/61‑1 (MQ=255)
ttCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGta < 1:3464288/61‑1 (MQ=255)
ttCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGta < 1:3743394/61‑1 (MQ=255)
ttCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGta < 1:1211630/61‑1 (MQ=255)
ttCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGta < 1:1908047/61‑1 (MQ=255)
ttCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGta < 1:4014246/61‑1 (MQ=255)
gAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:377964/70‑1 (MQ=255)
gAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:2811560/70‑1 (MQ=255)
gAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:3059707/70‑1 (MQ=255)
cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGatgatg < 1:2935628/71‑1 (MQ=255)
cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGatgatg < 1:492087/71‑1 (MQ=255)
cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGatgatg < 1:827676/71‑1 (MQ=255)
cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGatgatg < 1:3870062/71‑1 (MQ=255)
cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGatgatg < 1:357490/71‑1 (MQ=255)
cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGatgatg < 1:2770350/71‑1 (MQ=255)
cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGatgatg < 1:2365155/71‑1 (MQ=255)
cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGatgatg < 1:1919344/71‑1 (MQ=255)
cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGatgatg < 1:1405050/71‑1 (MQ=255)
cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGatgatg < 1:1066784/71‑1 (MQ=255)
gggCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGatgatg < 1:417657/67‑1 (MQ=255)
gcgagtgaTAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGatgatg < 1:1965402/65‑1 (MQ=255)
|
TTAATTAGCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGATGATG > W3110S.gb/515873‑515993
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
