Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I3 R2
|
122 |
64.1 |
3058059 |
97.6% |
2984665 |
63.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
1,013,547 |
G→A |
R245C (CGC→TGC) |
fumC ← |
fumarate hydratase (fumarase C), aerobic Class II |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,013,547 | 0 | G | A | 100.0%
| 155.8
/ NA
| 52 | R245C (CGC→TGC) | fumC | fumarate hydratase (fumarase C), aerobic Class II |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/31); total (21/31) |
CGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGC > minE/1013479‑1013613
|
cgcTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTacgcaac > 1:3025919/1‑71 (MQ=255)
gcTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTacgcaacg < 1:2553492/71‑1 (MQ=255)
cAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCa > 1:1322285/1‑71 (MQ=255)
aaaTTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCa > 1:1951836/1‑70 (MQ=255)
aaaTTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCa < 1:618728/70‑1 (MQ=255)
aaaTTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCa > 1:356045/1‑70 (MQ=255)
aTTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATAc > 1:16689/1‑71 (MQ=255)
tGTTCGGCGCGGTAACAAACAGTGCACAGGTAATGACTGCCAGTTCATCTGCTacgcaac > 1:2438075/1‑60 (MQ=255)
gTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCg > 1:1588092/1‑71 (MQ=255)
ttCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCgg < 1:2132342/71‑1 (MQ=255)
ggCGCGGTATCAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGAt < 1:2279520/70‑1 (MQ=255)
ggCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGAt < 1:1099967/70‑1 (MQ=255)
ggCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGAt < 1:2363764/70‑1 (MQ=255)
ggCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGAt < 1:2520364/70‑1 (MQ=255)
gcgcGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATgg > 1:128949/1‑71 (MQ=255)
gTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGAt < 1:2929969/64‑1 (MQ=255)
gTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTAtt < 1:528083/71‑1 (MQ=255)
gTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATAATCCGGATGGGTAtt < 1:2361639/71‑1 (MQ=255)
tAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTAtt > 1:251127/1‑70 (MQ=255)
tAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTAtt > 1:2059628/1‑70 (MQ=255)
aCAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAg < 1:1155707/71‑1 (MQ=255)
cAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGt > 1:1868614/1‑71 (MQ=255)
aaaCGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTc < 1:983848/71‑1 (MQ=255)
aaaCGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTc < 1:998198/71‑1 (MQ=255)
aaaCGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTc < 1:1180832/71‑1 (MQ=255)
aaCGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTcc < 1:1682595/71‑1 (MQ=255)
aCGGTGCACAGGTAATGACTGCCAGTTCATCTGCTacgcaacgag > 1:580358/1‑43 (MQ=255)
aCGGTGCACAGGTAATGACTGACAGTTCATCTTCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCa < 1:191290/71‑1 (MQ=255)
cGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCa < 1:411134/70‑1 (MQ=255)
gTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTa < 1:765725/71‑1 (MQ=255)
tGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTcc < 1:1719464/66‑1 (MQ=255)
cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCTGATGGGTATTTAGTCCAGTAccc < 1:986004/71‑1 (MQ=255)
cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTAccc < 1:1722157/71‑1 (MQ=255)
cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTAccc < 1:761504/71‑1 (MQ=255)
cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTAccc < 1:2191138/71‑1 (MQ=255)
ggTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCg < 1:791533/71‑1 (MQ=255)
ggTAATGACTGCCAGTTCATCTGCTACGCAAAGCGCATACTCCGGa > 1:852225/1‑46 (MQ=255)
gTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGc > 1:1156337/1‑71 (MQ=255)
aaTGACTGCCAGTTAATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTg > 1:2226118/1‑71 (MQ=255)
tGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTAtt < 1:2221543/49‑1 (MQ=255)
aGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTa > 1:2548899/1‑51 (MQ=255)
aGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGcc > 1:926558/1‑68 (MQ=255)
aGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGcc > 1:456182/1‑68 (MQ=255)
gTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTAtt < 1:2952482/40‑1 (MQ=255)
gTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTcc > 1:1678669/1‑46 (MQ=255)
gTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCaa > 1:2280514/1‑69 (MQ=255)
cATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGc < 1:192715/70‑1 (MQ=255)
cATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGc > 1:1949475/1‑70 (MQ=255)
aTCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGcc < 1:1108921/70‑1 (MQ=255)
aTCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGcc < 1:285884/70‑1 (MQ=255)
aTCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGcc < 1:2798708/70‑1 (MQ=255)
tGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGtt > 1:1710135/1‑71 (MQ=255)
tGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGtt > 1:1951200/1‑71 (MQ=255)
cTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCTCCAAGAGCCAg < 1:2499925/67‑1 (MQ=255)
cTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTcc < 1:2352807/71‑1 (MQ=255)
tACGCAACGCACATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCg > 1:2989911/1‑71 (MQ=255)
cgcaacgcgCATACTCCGGATGGGTATTTAGTCCAGTa < 1:2176853/38‑1 (MQ=255)
cgcaacgcgCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCg < 1:319568/69‑1 (MQ=255)
cgcaacgcgCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGc > 1:664349/1‑70 (MQ=255)
|
CGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGC > minE/1013479‑1013613
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
