Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R1 122 92.7 4382561 94.3% 4132755 66.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,509,579 G→A A42T (GCA→ACA)  ackA → acetate kinase A and propionate kinase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,509,5790GA100.0% 283.4 / NA 88A42T (GCA→ACA) ackAacetate kinase A and propionate kinase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (66/22);  total (66/22)

TCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGGCGCCGCT  >  minE/1509514‑1509641
                                                                 |                                                              
tcatcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGt                                                           <  1:1938297/71‑1 (MQ=255)
 catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGt                                                           >  1:2428949/1‑70 (MQ=255)
 catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGt                                                           >  1:1858000/1‑70 (MQ=255)
 catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:2413946/1‑71 (MQ=255)
 catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:3555796/1‑71 (MQ=255)
 catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:3463507/1‑71 (MQ=255)
 catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:3820556/1‑71 (MQ=255)
 catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:3159609/1‑71 (MQ=255)
 catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:3044583/1‑71 (MQ=255)
 catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:3963229/1‑71 (MQ=255)
 catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:3991060/1‑71 (MQ=255)
 catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:4335978/1‑71 (MQ=255)
 catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:1979094/1‑71 (MQ=255)
 catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:1750482/1‑71 (MQ=255)
 catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:1584427/1‑71 (MQ=255)
 catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:1365884/1‑71 (MQ=255)
 catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCC‑AATGTTTCCACCTGCCCGAAACACGTAt                                                         >  1:4059714/1‑71 (MQ=255)
  atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:3179997/1‑70 (MQ=255)
  atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:150389/1‑70 (MQ=255)
  atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTAt                                                         >  1:1617049/1‑71 (MQ=255)
  atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTAt                                                         >  1:3122529/1‑71 (MQ=255)
  atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTAt                                                         >  1:2747999/1‑71 (MQ=255)
  atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTAt                                                         >  1:806396/1‑71 (MQ=255)
  atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTAt                                                         >  1:2566066/1‑71 (MQ=255)
  atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTAt                                                         >  1:3110936/1‑71 (MQ=255)
  atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTAt                                                         >  1:3951673/1‑71 (MQ=255)
  atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTAt                                                         >  1:3949877/1‑71 (MQ=255)
  atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTAt                                                         >  1:145287/1‑71 (MQ=255)
  atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTAt                                                         >  1:341550/1‑71 (MQ=255)
  atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTAt                                                         >  1:451537/1‑71 (MQ=255)
  atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTAt                                                         >  1:3482910/1‑71 (MQ=255)
    cGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATc                                                        >  1:1937919/1‑70 (MQ=255)
    cGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATc                                                        >  1:1932916/1‑70 (MQ=255)
    cGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATc                                                        >  1:507841/1‑70 (MQ=255)
    cGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATc                                                        >  1:2737716/1‑70 (MQ=255)
           gTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatgga                                                 >  1:879246/1‑70 (MQ=255)
           gTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatgga                                                 >  1:4042920/1‑70 (MQ=255)
           gTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatgga                                                 >  1:2103034/1‑70 (MQ=255)
              aaTGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatggaaaa                                              <  1:1253500/70‑1 (MQ=255)
              aaTGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatggaaaa                                              <  1:319267/70‑1 (MQ=255)
              aaTGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatggaaaa                                              <  1:896078/70‑1 (MQ=255)
              aaTGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatggaaaa                                              <  1:1078260/70‑1 (MQ=255)
              aaTGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatggaaaa                                              <  1:1643180/70‑1 (MQ=255)
              aaTGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatggaaaa                                              <  1:2798729/70‑1 (MQ=255)
              aaTGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatggaaaa                                              <  1:1862877/70‑1 (MQ=255)
              aaTGGTGAAAAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatggaaaa                                              <  1:2571322/70‑1 (MQ=255)
               aTGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatggaaaatg                                            <  1:798496/71‑1 (MQ=255)
                          tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:3871286/1‑70 (MQ=255)
                          tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:3949944/1‑70 (MQ=255)
                          tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:3837900/1‑70 (MQ=255)
                          tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:613063/1‑70 (MQ=255)
                          tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:3099198/1‑70 (MQ=255)
                          tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:445328/1‑70 (MQ=255)
                          tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:943369/1‑70 (MQ=255)
                          tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:453568/1‑70 (MQ=255)
                          tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:958803/1‑70 (MQ=255)
                          tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:3401442/1‑70 (MQ=255)
                          tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:309731/1‑70 (MQ=255)
                          tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:135866/1‑70 (MQ=255)
                          tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:2841636/1‑70 (MQ=255)
                          tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:136502/1‑70 (MQ=255)
                          tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:1752688/1‑70 (MQ=255)
                          tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:2736861/1‑70 (MQ=255)
                          tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:1774259/1‑70 (MQ=255)
                          tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:259551/1‑70 (MQ=255)
                                            gAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAgg               <  1:3803675/71‑1 (MQ=255)
                                            gAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAgg               <  1:1135884/71‑1 (MQ=255)
                                            gAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAgg               <  1:1311872/71‑1 (MQ=255)
                                            gAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAgg               <  1:766101/71‑1 (MQ=255)
                                            gAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAgg               <  1:705077/71‑1 (MQ=255)
                                            gAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAgg               <  1:548416/71‑1 (MQ=255)
                                            gAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAgg               <  1:2337208/71‑1 (MQ=255)
                                            gAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAgg               <  1:4288864/71‑1 (MQ=255)
                                            gAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAgg               <  1:3973113/71‑1 (MQ=255)
                                            gAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAgg               <  1:3916127/71‑1 (MQ=255)
                                            gAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAgg               <  1:3175227/71‑1 (MQ=255)
                                              aTGTTTCCACCTGCCCGAAACACGTATCaaatggaaaa                                              <  1:3987126/38‑1 (MQ=255)
                                                   tCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAgg               >  1:2687942/1‑64 (MQ=255)
                                                   tCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg         >  1:2386526/1‑70 (MQ=255)
                                                   tCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAg          >  1:3738446/1‑69 (MQ=255)
                                                   tCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGgc        >  1:434446/1‑71 (MQ=255)
                                                   tCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGgc        >  1:129323/1‑71 (MQ=255)
                                                   tCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGgc        >  1:1226449/1‑71 (MQ=255)
                                                         tGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGGCGCCGCt  >  1:1507107/1‑71 (MQ=255)
                                                         tGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGGCGCCGCt  >  1:2765591/1‑71 (MQ=255)
                                                         tGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGGCGCCGCt  >  1:2877399/1‑71 (MQ=255)
                                                         tGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGGCGCCGCt  >  1:2882669/1‑71 (MQ=255)
                                                         tGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGGCGCCGCt  >  1:1172389/1‑71 (MQ=255)
                                                                 |                                                              
TCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGGCGCCGCT  >  minE/1509514‑1509641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A