Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F21 I0 R1
|
57 |
0.0 |
661283 |
90.4% |
597799 |
68.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,325,522 |
T→G |
100% |
K71T (AAA→ACA) |
wcaL ← |
predicted glycosyl transferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,325,522 | 0 | T | G | 100.0%
| 29.4
/ NA
| 9 | K71T (AAA→ACA) | wcaL | predicted glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (0/9); total (0/9) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTG > minE/1325508‑1325578
|
ctCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTg < 1:110222/71‑1 (MQ=255)
ctCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTg < 1:142473/71‑1 (MQ=255)
ctCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTg < 1:175256/71‑1 (MQ=255)
ctCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTg < 1:185874/71‑1 (MQ=255)
ctCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTg < 1:286012/71‑1 (MQ=255)
ctCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTg < 1:360412/71‑1 (MQ=255)
ctCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTg < 1:365721/71‑1 (MQ=255)
ctCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTg < 1:447572/71‑1 (MQ=255)
ctCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTg < 1:493115/71‑1 (MQ=255)
|
CTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTG > minE/1325508‑1325578
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
