Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I1 R2
|
146 |
74.7 |
3299319 |
84.8% |
2797822 |
66.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
1,325,522 |
T→G |
K71T (AAA→ACA) |
wcaL ← |
predicted glycosyl transferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,325,522 | 0 | T | G | 100.0%
| 16.9
/ NA
| 6 | K71T (AAA→ACA) | wcaL | predicted glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (0/6); total (0/6) |
CCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCG > minE/1325489‑1325559
|
ccGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCg < 1:1015066/71‑1 (MQ=255)
ccGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCg < 1:1510532/71‑1 (MQ=255)
ccGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCg < 1:1794849/71‑1 (MQ=255)
ccGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCg < 1:338216/71‑1 (MQ=255)
ccGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCg < 1:394378/71‑1 (MQ=255)
ccGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCg < 1:540160/71‑1 (MQ=255)
|
CCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCG > minE/1325489‑1325559
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
