Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F17 I0 R1
|
71 |
95.7 |
2922988 |
91.7% |
2680379 |
65.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,325,522 |
T→G |
100% |
K71T (AAA→ACA) |
wcaL ← |
predicted glycosyl transferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,325,522 | 0 | T | G | 100.0%
| 57.2
/ NA
| 16 | K71T (AAA→ACA) | wcaL | predicted glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (0/16); total (0/16) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGT > minE/1325468‑1325538
|
cAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGt < 1:1009614/71‑1 (MQ=255)
cAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGt < 1:1054722/71‑1 (MQ=255)
cAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGt < 1:1455894/71‑1 (MQ=255)
cAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGt < 1:1806970/71‑1 (MQ=255)
cAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGt < 1:1854123/71‑1 (MQ=255)
cAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGt < 1:2017513/71‑1 (MQ=255)
cAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGt < 1:2148852/71‑1 (MQ=255)
cAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGt < 1:2166052/71‑1 (MQ=255)
cAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGt < 1:2205175/71‑1 (MQ=255)
cAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGt < 1:2268067/71‑1 (MQ=255)
cAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGt < 1:2547001/71‑1 (MQ=255)
cAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGt < 1:2561940/71‑1 (MQ=255)
cAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGt < 1:284004/71‑1 (MQ=255)
cAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGt < 1:389887/71‑1 (MQ=255)
cAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGt < 1:805205/71‑1 (MQ=255)
aGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGt < 1:1915132/70‑1 (MQ=255)
|
CAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGT > minE/1325468‑1325538
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
