Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R1
|
311 |
50.1 |
4085234 |
95.9% |
3917739 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,065,306 |
A→G |
24.1% |
V175A (GTT→GCT) |
ygfI ← |
predicted DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,065,306 | 0 | A | G | 24.1%
| 96.3
/ 32.4
| 54 | V175A (GTT→GCT) | ygfI | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (17/24); new base G (5/8); total (22/32) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01 |
CGCATTTGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGA > W3110S.gb/3065240‑3065376
|
cgcATTTGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGcgagaaacgag < 1:3185468/71‑1 (MQ=255)
ccAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGcgagaaacg < 1:486315/58‑1 (MQ=255)
ccAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGcgagaaacg < 1:481452/58‑1 (MQ=255)
ccAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGcgagaaacg < 1:1860068/58‑1 (MQ=255)
cAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCg > 1:716923/1‑71 (MQ=255)
cTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtgg < 1:168785/69‑1 (MQ=255)
cTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTa < 1:927684/71‑1 (MQ=255)
aaGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAg < 1:365879/69‑1 (MQ=255)
aaGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGtt < 1:34662/71‑1 (MQ=255)
cagcTCTTGCTCTTGTCGCTCTTCATAACGGcgagaaa > 1:605714/1‑38 (MQ=255)
cagcTCTTGCTCTTGTCGCTCTTCATAACGGcgagaaa > 1:2811443/1‑38 (MQ=255)
cagcTCTTGCTCTTGTCGCTCTTCATAACGGcgagaaa > 1:2328345/1‑38 (MQ=255)
cagcTCTTGCTCTTGTCGCTCTTCATAACGGcgagaaa > 1:3574101/1‑38 (MQ=255)
ctcttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGa < 1:801021/71‑1 (MQ=255)
cttgctcttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGa > 1:2773614/1‑69 (MQ=255)
cttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggt > 1:2837375/1‑56 (MQ=255)
cttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggt > 1:2808103/1‑56 (MQ=255)
cttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGa > 1:1939757/1‑69 (MQ=255)
cttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGa > 1:2992657/1‑69 (MQ=255)
cttgctattgTCGCTCTTCATAACTGCGAGAAACGAGAAAGCCTAATCGCAGCggt > 1:2297293/1‑56 (MQ=255)
ttgctcttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCggtgg < 1:749165/57‑1 (MQ=255)
ttgctcttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAg > 1:3856635/1‑71 (MQ=255)
ttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAAt < 1:804983/46‑1 (MQ=255)
ttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGc < 1:3782959/49‑1 (MQ=255)
ttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGc < 1:3006012/52‑1 (MQ=255)
ttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGc < 1:1682911/52‑1 (MQ=255)
ttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGc < 1:519911/52‑1 (MQ=255)
ttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAg > 1:3258299/1‑71 (MQ=255)
gctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCgg > 1:1559097/1‑52 (MQ=255)
gctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCgg > 1:1492519/1‑52 (MQ=255)
gctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCgg > 1:2564006/1‑52 (MQ=255)
gctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCgg > 1:2630869/1‑52 (MQ=255)
tcttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGtt < 1:3216757/58‑1 (MQ=255)
ttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTc < 1:3859931/57‑1 (MQ=255)
ttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTc < 1:1087096/57‑1 (MQ=255)
ttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTc < 1:182942/57‑1 (MQ=255)
ttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGt > 1:2414129/1‑71 (MQ=255)
gTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTaa > 1:2895098/1‑37 (MQ=255)
gTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGTTGGTAGTTCACTGATAGTCAgg < 1:1529836/68‑1 (MQ=255)
gTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAgg < 1:3662455/68‑1 (MQ=255)
gTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAgg < 1:3251922/68‑1 (MQ=255)
gTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAgg < 1:1813381/68‑1 (MQ=255)
ttCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATa < 1:2106199/55‑1 (MQ=255)
ttCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATa < 1:3219454/55‑1 (MQ=255)
ttCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGc < 1:3348613/37‑1 (MQ=255)
ttCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGc < 1:3638801/37‑1 (MQ=255)
ttCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGc < 1:2272175/37‑1 (MQ=255)
ttCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGtt < 1:1884287/47‑1 (MQ=255)
ttCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCa < 1:2354817/49‑1 (MQ=255)
ttCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTcc < 1:230168/67‑1 (MQ=255)
cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCgg > 1:346334/1‑37 (MQ=255)
cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTcc > 1:2065775/1‑71 (MQ=255)
cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTcc > 1:1718274/1‑71 (MQ=255)
aaTAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTcc > 1:618847/2‑65 (MQ=255)
tAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTc < 1:3958962/68‑1 (MQ=255)
tAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTc < 1:1293108/68‑1 (MQ=255)
tAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTc < 1:1512101/68‑1 (MQ=255)
tAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTg < 1:2928512/71‑1 (MQ=255)
tAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTACAGTTCCTg < 1:3635070/71‑1 (MQ=255)
aCGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAg > 1:3991367/1‑63 (MQ=255)
aCGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAg > 1:766193/1‑63 (MQ=255)
aCGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTc > 1:3900177/1‑66 (MQ=255)
agaaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTg < 1:3182154/71‑1 (MQ=255)
agaaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTg < 1:2970435/71‑1 (MQ=255)
aaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCa < 1:3890914/71‑1 (MQ=255)
aaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCa < 1:300048/71‑1 (MQ=255)
aaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCa < 1:291700/71‑1 (MQ=255)
aaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCa < 1:2400100/71‑1 (MQ=255)
aacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTcc < 1:2439935/52‑1 (MQ=255)
acgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGa < 1:2095658/71‑1 (MQ=255)
acgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGa < 1:1903108/71‑1 (MQ=255)
|
CGCATTTGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGA > W3110S.gb/3065240‑3065376
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
