Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F17 I0 R2
|
276 |
45.3 |
3729697 |
93.0% |
3468618 |
60.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,065,306 |
A→G |
16.7% |
V175A (GTT→GCT) |
ygfI ← |
predicted DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,065,306 | 0 | A | G | 16.7%
| 127.7
/ 19.3
| 54 | V175A (GTT→GCT) | ygfI | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (27/18); new base G (6/3); total (33/21) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 |
TGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACT > W3110S.gb/3065246‑3065368
|
tGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAg > 1:1238816/1‑69 (MQ=255)
tGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAg > 1:3183787/1‑69 (MQ=255)
tGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAg > 1:1551718/1‑69 (MQ=255)
cAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg > 1:2207959/1‑69 (MQ=255)
cAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg > 1:2133740/1‑69 (MQ=255)
cAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAg > 1:3332464/1‑69 (MQ=255)
tCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCgg > 1:1249978/1‑68 (MQ=255)
ccTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggt > 1:648174/1‑68 (MQ=255)
ccTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggt > 1:28990/1‑68 (MQ=255)
gAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtggt < 1:2468280/68‑1 (MQ=255)
gAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtggt < 1:1750213/68‑1 (MQ=255)
gAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtggt < 1:485078/68‑1 (MQ=255)
gAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtggt < 1:3544371/68‑1 (MQ=255)
gAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtggt < 1:1007378/68‑1 (MQ=255)
gAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtggt < 1:11610/68‑1 (MQ=255)
gAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtggt < 1:368087/68‑1 (MQ=255)
gAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtgg < 1:667205/67‑1 (MQ=255)
gAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTa < 1:2673238/69‑1 (MQ=255)
gcagcTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGtt < 1:3646194/69‑1 (MQ=255)
cagcTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAg < 1:884751/66‑1 (MQ=255)
agcTCTTGCTCTTGTCGCTCTTCATAACGGcgagaaacgag < 1:3277954/41‑1 (MQ=255)
cttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtgg > 1:132270/1‑58 (MQ=255)
cttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtgg > 1:2255003/1‑58 (MQ=255)
cttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtgg > 1:2318337/1‑58 (MQ=255)
cttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtgg < 1:2462152/58‑1 (MQ=255)
cttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACt > 1:2157871/1‑67 (MQ=255)
tgctcttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATa > 1:3725999/1‑69 (MQ=255)
gctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGtt > 1:817422/1‑60 (MQ=255)
ctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGa > 1:2964315/1‑65 (MQ=255)
cttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCa < 1:366104/69‑1 (MQ=255)
cttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGATCACTGATAGTCa < 1:3241188/69‑1 (MQ=255)
ttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCggtggt < 1:1612633/52‑1 (MQ=255)
gTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAg < 1:3377142/67‑1 (MQ=255)
gTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGtt > 1:2511401/1‑54 (MQ=255)
gTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGtt > 1:270081/1‑54 (MQ=255)
gTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGt > 1:697120/1‑69 (MQ=255)
ctctTCATAACGGCGAGAAACGAGAAAGCCTAATCGGAGCggtgg < 1:1930979/45‑1 (MQ=255)
ctctTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtgg < 1:1261896/45‑1 (MQ=255)
ctctTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCggtgg < 1:412116/45‑1 (MQ=255)
cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACt < 1:2558953/49‑1 (MQ=255)
cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACt > 1:1132690/1‑49 (MQ=255)
cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACt > 1:3364848/1‑49 (MQ=255)
cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACt < 1:1697260/49‑1 (MQ=255)
cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTcc > 1:1303711/1‑65 (MQ=255)
cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTcc > 1:2884506/1‑65 (MQ=255)
cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTcc > 1:2163689/1‑65 (MQ=255)
cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGtt > 1:588101/1‑69 (MQ=255)
cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGtt > 1:99387/1‑69 (MQ=255)
cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGtt > 1:3175273/1‑69 (MQ=255)
cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGtt > 1:2425111/1‑69 (MQ=255)
cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGtt > 1:1217974/1‑69 (MQ=255)
cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGACAGGTTTTCCAGtt > 1:3283906/1‑69 (MQ=255)
cATAACGGCGAGAAACGAGAAAGCCTAATCACAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCAAGtt > 1:2148521/1‑69 (MQ=255)
aCGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCa > 1:892583/1‑53 (MQ=255)
aCGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCa > 1:1179627/1‑53 (MQ=255)
cgagaaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACt < 1:512981/69‑1 (MQ=255)
cgagaaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACt < 1:128712/69‑1 (MQ=255)
agaagcgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTc < 1:1332734/54‑1 (MQ=255)
acgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGt > 1:2641475/1‑54 (MQ=255)
|
TGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACT > W3110S.gb/3065246‑3065368
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
