Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F129 I1 R1
|
16 |
78.2 |
4038333 |
91.8% |
3707189 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,196,331 |
C→G |
intergenic (‑232/+1) |
thiC ← / ← rsd |
phosphomethylpyrimidine synthase/stationary phase protein, binds sigma 70 RNA polymerase subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,196,331 | 0 | C | G | 96.9%
| 236.1
/ ‑0.6
| 65 | intergenic (‑232/+1) | thiC/rsd | phosphomethylpyrimidine synthase/stationary phase protein, binds sigma 70 RNA polymerase subunit |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base G (34/29); total (35/30) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 |
CCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTC > NC_000913/4196235‑4196424
|
cccGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCa < 2:617621/100‑1 (MQ=255)
cccGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCACCGCAAATGGGGAATTGAATGTAAATTACGAGTTAACAGCGCAGAAGTCa > 2:482629/1‑100 (MQ=255)
cGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAg > 2:798762/1‑100 (MQ=255)
gAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGc > 2:1304132/1‑100 (MQ=255)
gAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGc < 2:1391738/100‑1 (MQ=255)
gAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGc < 1:1491841/100‑1 (MQ=255)
gAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGc > 1:1733547/1‑100 (MQ=255)
aaTAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCa > 2:635060/1‑100 (MQ=255)
aaaCGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGa > 2:462563/1‑100 (MQ=255)
ggTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGtt < 1:806951/100‑1 (MQ=255)
gTCTCANCCAGTTAAGGCACTCCGACAAGAAAGTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGNGTTANCAGCGCAGAAGTCAAGCAGGATGttt < 1:1576608/100‑1 (MQ=255)
gTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGttt > 2:995840/1‑100 (MQ=255)
tctcAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTg < 2:490700/100‑1 (MQ=255)
cAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGAcg < 2:1574832/100‑1 (MQ=255)
gCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGAcgcg > 2:1882082/1‑100 (MQ=255)
gCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGAcgcg > 2:225869/1‑100 (MQ=255)
ccAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACgcgg > 2:1829329/1‑100 (MQ=255)
gTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACgcgggcg < 2:1805031/100‑1 (MQ=255)
aaGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGgcggcg > 1:317489/1‑100 (MQ=255)
aaGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGgcggcg < 2:1855398/100‑1 (MQ=255)
aaGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGgcggcg < 1:1286127/100‑1 (MQ=255)
aaGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGgcggcg < 1:984138/100‑1 (MQ=255)
gCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCa < 1:1599263/100‑1 (MQ=255)
aCTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAg < 1:1304138/100‑1 (MQ=255)
aCTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAg < 2:1296794/100‑1 (MQ=255)
aCTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAg < 1:995843/100‑1 (MQ=255)
cTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGc < 1:462562/100‑1 (MQ=255)
tCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAAGTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCa < 1:1884958/100‑1 (MQ=255)
ccGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCAc > 2:1971793/1‑100 (MQ=255)
ccGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCAc < 1:798764/100‑1 (MQ=255)
cGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCAcc > 1:250472/1‑100 (MQ=255)
gACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACca > 1:1986278/1‑100 (MQ=255)
aCAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACcag < 1:1238321/100‑1 (MQ=255)
aCAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACcag < 1:194296/100‑1 (MQ=255)
aaGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACcagca > 2:1049710/1‑100 (MQ=255)
aaGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACcagca < 2:98170/100‑1 (MQ=255)
aaGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACcagca > 2:1981089/1‑100 (MQ=255)
gAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGa > 2:1046606/1‑100 (MQ=255)
ccccGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCtt > 2:302414/1‑100 (MQ=255)
ccGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTAt < 2:1733535/100‑1 (MQ=255)
ggggCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGc < 2:953443/100‑1 (MQ=255)
gggCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGca > 2:1585765/1‑100 (MQ=255)
ggCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGcac > 1:498112/1‑100 (MQ=255)
ggCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGcac > 2:409805/1‑100 (MQ=255)
gCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGcaca > 2:263281/1‑100 (MQ=255)
ttGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAg > 1:1230190/1‑100 (MQ=255)
gAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAgcg < 2:1532116/100‑1 (MQ=255)
gAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAgcg > 2:1932153/1‑100 (MQ=255)
gAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAgcg > 1:1633713/1‑100 (MQ=255)
gAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAgcg > 1:812738/1‑100 (MQ=255)
aTGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAgcgcg > 1:1122641/1‑100 (MQ=255)
gTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCt > 2:99944/1‑100 (MQ=255)
gTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCt < 1:350957/100‑1 (MQ=255)
tAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCtt > 1:1377070/1‑100 (MQ=255)
aaTTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTcc > 2:344585/1‑100 (MQ=255)
tACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAg > 2:1274219/1‑99 (MQ=255)
gcgTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGcc < 1:819308/100‑1 (MQ=255)
gcgTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGcc < 1:765441/100‑1 (MQ=255)
gcgTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGcc < 2:323706/100‑1 (MQ=255)
cgTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCt > 2:727547/1‑100 (MQ=255)
aaCAGCGCAGAAGTCTAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGcc < 1:1981101/100‑1 (MQ=255)
aaCAGCGCAGAAGTCAAGCAGGATGTTTGACGCTGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGcc > 2:1313409/1‑100 (MQ=255)
aCAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCg < 1:471237/100‑1 (MQ=255)
cAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGa > 2:1487875/1‑100 (MQ=255)
gcAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTc > 1:1536512/1‑100 (MQ=255)
|
CCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTC > NC_000913/4196235‑4196424
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
