breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
JC 147,727 Δ12 bp coding (760‑771/903 nt) yadD → transposase_31 family protein
MC JC 257,908 Δ776 bp [crl] [crl]
RA 1,143,998 C→T G124S (GGT→AGT)  rne ← endoribonuclease; RNA‑binding protein;RNA degradosome binding protein
JC JC 1,293,033 IS1 (+) +9 bp intergenic (‑111/‑486) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,866,903 C→T E217K (GAG→AAG)  rpoS ← RNA polymerase, sigma S (sigma 38) factor
RA 2,917,345 G→A G763G (GGG→GGA barA → hybrid sensory histidine kinase, in two‑component regulatory system with UvrY
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,566,544 C→A S13I (AGC→ATC)  glgP ← glycogen phosphorylase
MC JC 3,815,859 Δ82 bp [rph][rph] [rph], [rph]
RA 4,027,058 C→A R178S (CGT→AGT)  fre → NAD(P)H‑flavin reductase
RA 4,196,331 C→G intergenic (‑232/+1) thiC ← / ← rsd phosphomethylpyrimidine synthase/stationary phase protein, binds sigma 70 RNA polymerase subunit
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
JC 4,628,231 Δ14 bp coding (917‑930/1233 nt) nadR → trifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423706–3424234 3424585–3424239 6–880 35 [33] [33] 34 [rrfD]–[rrlD] [rrfD], [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =4 (0.050)64 (0.970) 52/166 0.2 96.7% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 1 (0.020)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12078054 (0.050)62 (0.940) 42/166 0.6 96.6% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 1 (0.020)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)92 (1.220) 62/190 0.1 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein