Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F112 I0 R1	51	112.5	5500062	95.1%	5230558	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	756,180	G→T	100%	G92W (GGG→TGG)	sdhA →	succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	756,180	0	G	T	92.2%	291.3 / 14.3	103	G92W (GGG→TGG)	sdhA	succinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand): ref base G (5/4); new base T (40/53); total (45/58)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 103 total aligned reads displayed.

ACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTC  >  NC_000913/756084‑756274

aCCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGc                                                                                             >  1:1729949/1‑100 (MQ=255)

aCCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGc                                                                                             <  1:1106985/100‑1 (MQ=255)

aCCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGc                                                                                             <  2:808219/100‑1 (MQ=255)

 gccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGcc                                                                                            >  1:1431745/2‑100 (MQ=255)

 cccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGcc                                                                                            >  1:2722502/1‑100 (MQ=255)

  ccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCg                                                                                           >  1:1387029/1‑100 (MQ=255)

   cATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGCCGCGATTGAATATATGTGTAAAACCTGGCCgg                                                                                          <  1:2607840/100‑1 (MQ=255)

   cATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCgg                                                                                          >  2:93386/1‑100 (MQ=255)

   cATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCgg                                                                                          >  1:1373029/1‑100 (MQ=255)

     tGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGaa                                                                                        <  2:1705908/100‑1 (MQ=255)

      gAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAg                                                                                       <  1:946497/100‑1 (MQ=255)

      gAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAg                                                                                       <  1:2120860/100‑1 (MQ=255)

       aaGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGc                                                                                      <  2:918780/100‑1 (MQ=255)

        aGATAAGTGGGGATGGCATATGTACGATGCGGTTAATGGGTGGGACTAGATAGGTAAAAATGACACGATTGGAGATATGTGTAAAGCCGGGCAGGAAg                                                                                       <  2:2115308/98‑1 (MQ=255)

        aGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCg                                                                                     <  2:2264195/100‑1 (MQ=255)

           tAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGAtt                                                                                  >  2:323856/1‑100 (MQ=255)

           tAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATATGTAAAAGCTGGCCGGAAGCGAtt                                                                                  >  2:384631/1‑100 (MQ=255)

           tAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGACTGAATATACGTGTAAAACCTGTCCGGGAGCGa                                                                                    >  2:293054/1‑98 (MQ=255)

             aCTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCt                                                                                <  2:459284/100‑1 (MQ=255)

             aCTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCt                                                                                >  2:1358562/1‑100 (MQ=255)

             aCTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCt                                                                                >  1:177552/1‑100 (MQ=255)

               tGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTgg                                                                              <  1:2060587/100‑1 (MQ=255)

                gggAATGGCATATGTACGACACCGTGAAAGGTTTGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAATCCTGGCCGGAAGCGATTCTGGa                                                                             <  2:383114/100‑1 (MQ=255)

                gggAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTggn                                                                             <  1:25888/100‑2 (MQ=255)

                  gAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAAc                                                                           <  2:1963353/100‑1 (MQ=255)

                  gAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAAc                                                                           >  1:1195062/1‑100 (MQ=255)

                  gAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAAc                                                                           <  1:372360/100‑1 (MQ=255)

                      ggCATATGTACGACACCGTGGAAGGGTCGGACTATATCGGTGACCAGGGCGCGATTGAATATATGTATAAAACCTGGCCGGAGGCGATTCTGGCAc                                                                           >  2:1679773/1‑96 (MQ=255)

                      ggCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGa                                                                       <  2:288177/100‑1 (MQ=255)

                      ggCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGa                                                                       <  1:2351589/100‑1 (MQ=255)

                        cATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAac                                                                     <  2:523896/100‑1 (MQ=255)

                         atatGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGCTTCTGGAACTCGAaca                                                                    >  2:1083128/1‑100 (MQ=255)

                         atatGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAaca                                                                    <  2:2544781/100‑1 (MQ=255)

                         atatGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAaca                                                                    >  1:1399003/1‑100 (MQ=255)

                         atatGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAac                                                                     <  2:1917217/99‑1 (MQ=255)

                          tatGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAacac                                                                   <  2:1099579/100‑1 (MQ=255)

                           atGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAAcaca                                                                  >  2:2081938/1‑100 (MQ=255)

                           atGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTCGAACTCGAacaaa                                                                  <  1:1864738/100‑3 (MQ=255)

                            tGTACGACACCGTGAAAGGGTCGGACTATATCGGTGAACAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCTAACACAt                                                                 >  2:1471396/1‑100 (MQ=255)

                              tACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATg                                                                <  1:1067958/99‑1 (MQ=255)

                               aCGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATggg                                                              <  1:1050413/100‑1 (MQ=255)

                               aCGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATggg                                                              <  2:660765/100‑1 (MQ=255)

                               aCGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATggg                                                              <  1:876871/100‑1 (MQ=255)

                               aCGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATggg                                                              <  1:1121479/100‑1 (MQ=255)

                               aCGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATggg                                                              >  1:696920/1‑100 (MQ=255)

                               aCGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATggg                                                              >  1:187369/1‑100 (MQ=255)

                                 gACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGcc                                                            <  1:323856/100‑1 (MQ=255)

                                  acacCGTGAAAGGGTCGGACTATATCGGTGACCNGGACGCGCTTGAATATCTGTGTAAAACCGGGCCGGAAGCGCTTCTCGCACTCGAACACACGGGcc                                                            >  2:2515688/1‑99 (MQ=255)

                                   cacCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTACAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTg                                                          >  2:1764027/1‑100 (MQ=255)

                                     ccGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGcc                                                        >  1:96867/1‑100 (MQ=255)

                                     ccGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGcc                                                        >  1:1274485/1‑100 (MQ=255)

                                     ccGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGcc                                                        <  2:2507740/100‑1 (MQ=255)

                                      cGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCg                                                       >  1:1006081/1‑100 (MQ=255)

                                        tGAAAGGGTCGGACCATCTCGGTGACCAGGACGCGATTGAATATCTGTGTAAATCCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGtt                                                     <  1:1888055/100‑1 (MQ=255)

                                         gAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTtc                                                    <  1:921814/100‑1 (MQ=255)

                                           aaGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTtctc                                                  >  1:574754/1‑100 (MQ=255)

                                           aaGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTtctc                                                  <  2:1219891/100‑1 (MQ=255)

                                             gggTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGAGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTcg                                                 >  2:1324322/1‑99 (MQ=255)

                                              ggTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTcgcg                                               <  1:93386/100‑1 (MQ=255)

                                                  ggACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGtctc                                           >  1:475754/1‑100 (MQ=255)

                                                     cTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCgat                                        <  2:1629269/100‑1 (MQ=255)

                                                       atatCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCgat                                        <  2:1567242/98‑1 (MQ=255)

                                                         atCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGCCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCgat                                        <  2:1435317/96‑1 (MQ=255)

                                                          tCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATgg                                   <  2:2530994/100‑1 (MQ=255)

                                                            ggTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTc                                 <  1:2437628/100‑1 (MQ=255)

                                                             gTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACACGGGCCCGCCGTTCTCGCGTCTCGATGATGGTCg                                <  1:384631/100‑1 (MQ=255)

                                                                 ccAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatc                            >  1:759828/1‑100 (MQ=255)

                                                                 ccAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatc                            >  2:1576932/1‑100 (MQ=255)

                                                                   aGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatcta                          <  1:117519/100‑1 (MQ=255)

                                                                   aGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatcta                          >  1:2108827/1‑100 (MQ=255)

                                                                    ggACGCGATTGAAGATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatctat                         >  1:2409010/1‑100 (MQ=255)

                                                                     gACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatctatc                        <  2:2645313/100‑1 (MQ=255)

                                                                       cgcgATTGAATATATGTGTAAAGCCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCaa                      <  2:32045/100‑1 (MQ=255)

                                                                        gcgATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAAc                     <  1:1382180/100‑1 (MQ=255)

                                                                         cgATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACg                    >  1:1071684/1‑100 (MQ=255)

                                                                         cgATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACg                    <  1:2175216/100‑1 (MQ=255)

                                                                         cgATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACg                    >  2:1099364/1‑100 (MQ=255)

                                                                          gATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGt                   >  1:320488/1‑100 (MQ=255)

                                                                          gATTGAATATAGGTGTAAAACCTGGCCGGAAGCGATTCTGGAGCTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACg                    <  2:1778494/99‑1 (MQ=255)

                                                                            ttGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTcc                 >  2:1004810/1‑100 (MQ=255)

                                                                             tGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCg                <  2:2284864/100‑1 (MQ=255)

                                                                             tGAAGATATGTGTAAAACCAGGCCGGAAGCGATTCTGAAACTCGAACAGATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCg                <  2:1525651/100‑1 (MQ=255)

                                                                              gAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGATACTCGAACACATGCGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCg                <  2:573905/99‑1 (MQ=255)

                                                                                atatatGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGttt             <  1:194440/100‑1 (MQ=255)

                                                                                atatatGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGttt             <  1:1294992/100‑1 (MQ=255)

                                                                                 tatatGTGTAAAGCCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTg            <  2:1744640/100‑1 (MQ=255)

                                                                                 tatatGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTg            >  2:776155/1‑100 (MQ=255)

                                                                                 tatatGTCTAAATCCTGGCGGGAAGCGTTTCTGGAATTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATTGTCGTATCTATCAACGTCCg                <  2:441635/96‑1 (MQ=255)

                                                                                   tatGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGc          <  1:2349939/100‑1 (MQ=255)

                                                                                   tatGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGc          <  1:518114/100‑1 (MQ=255)

                                                                                    ctgtgTAAAACCTGGCTGGATGCGCTTGTGGAACTAGAATACTTGGGCCTGTCGTTCTTGCGTCTGGATGATGGTCGTATCTATCAACGTGCGTTGGGCg         <  2:912299/99‑1 (MQ=255)

                                                                                     tgtgTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCgg        >  2:346129/1‑100 (MQ=255)

                                                                                      gtgtAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACTTCCGTTTGGCGGt       >  2:508542/1‑100 (MQ=255)

                                                                                       tgtAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTc      >  2:236833/1‑100 (MQ=255)

                                                                                       tgtAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTc      <  1:2552020/100‑1 (MQ=255)

                                                                                        gtAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCa     >  2:314560/1‑100 (MQ=255)

                                                                                        gtAAAACCTGGCCGGAAGCAATTCTGGAACTCGAACACATGGGCCTGCCGATCTAGCGTCTCGATGATTGCAGTATCTATCAACGTCCGTTTTGCGGTCa     >  2:2243139/1‑100 (MQ=255)

                                                                                        gtAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTc      <  2:1208886/99‑1 (MQ=255)

                                                                                         tAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAg    >  2:766255/1‑100 (MQ=255)

                                                                                           aaaCCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTc  <  1:162151/100‑1 (MQ=255)

ACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTC  >  NC_000913/756084‑756274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A